Abstract
Relatively little is known about the histogenesis and the underlying mechanisms of lung cancer; in particular about the target cell type(s) of carcinogens (Schüller 1987). The same can be said about the mechanisms of epithelial regeneration in the lung after injury by toxic chemicals. One of the morphologically appreciable responses of the lung to such injury is a hyperplastic growth of bronchiolo-alveolar epithelium. There is speculation that type II pneumocytes or Clara cells may give rise to the hyperplasia. Neuroendocrine (APUD) cells and type I pneumocytes are supposed to be less capable of initiating hyperplasia because of their low activity of cell division, although hyperplasia of the former cell type is experimentally inducible (Schuller et al. 1988). Mutual transformation has even been postulated between type II pneumocytes and Clara cells in certain cases (Schüller 1987). There is another cell type which, occurring in a fetal as well as regenerating adult airway epithelium (McDowell et al. 1984; Emura 1985; Strum et al. 1990), closely resembles the small mucus granule cells (SMGC) postulated to be one of the carcinogen targets in the lung (McDowell and Trump 1983). In our cell culture study (Emura et al. 1990) a large population of cells possessing SMGC characteristics are inducible in vitro from an undifferentiated population of airway epithelial cells.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Brockmeyer C, Emura M, Ochiai A, Riebe M, Mohr U, Hilfrich J (1990) Secretory differentiation of human fetal bronchial epithelial cells in culture. A study by histochemistry and electron microscopy. Exp Pathol 39:11–17
Conradt P, Green U (1982) Effect of diazepam on microsomal diethyl- nitrosamine metabolism in gerbils. J Cancer Res Clin Oncol 104:53–61
Emura M (1985) Development of Syrian golden hamster tracheal epithelium during prenatal and immediate postnatal stages. In: Jones TC, Mohr U, Hunt RD (eds) Respiratory system. Springer Berlin Heidelberg New York pp 11–27
Emura M, Riebe M, Ochiai A, Aufderheide M, Germann P, Mohr U (1990) New functional cell-culture approach to pulmonary carcinogenesis and toxicology. J Cancer Res Clin Oncol 116:557–562
Jacob J, Grimmer G, Raab G, Emura M, Riebe M, Mohr U (1990) Metabolism of pyrene and chrysene in epithelial human bronchial and hamster lung cells. Int J Environ Anal Chem 38:221–230
Katyal SL, Estes LW, Lombardi B (1977) Method for the isolation of surfactant from homogenates and lavages of lung of adult, newborn, and fetal rats. Lab Invest 36:585–592
McDowell EM, Keenan KP, Huang M (1984) Effects of vitamin A- deprivation on hamster tracheal epithelium. A quantitative morphologic study. Virchows Arch B 45:197–219
McDowell EM, Trump BF (1983) Histogenesis of preneoplastic and neoplastic lesions in tracheobronchial epithelium. Surv Synth Pathol Res 2:235–279
Ochiai A, Emura M, Riebe-Imre M, Mohr U, Hilfrich J, Tahara E, Dungworth DL (1991) Secretory differentiation and cell type identification of a human fetal bronchial epithelial cell line (HFBE). Virchows Arch B in press
Schüller HM (1987) Experimental carcinogenesis in the peripheral lung. In: McDowell EM (ed) Lung carcinomas. Churchill Livingstone, Edinburgh London Melbourne New York pp 243–254
Schuller HM, Becker KL, Witschi H-P (1988) An animal model for neuroendocrine lung cancer. Carcinogenesis 9:293–296
Singh G, Katyal SL, Ward JM, Gottron SA, Wong-Chong M-L, Riley EJ (1985) Secretory proteins of the lung in rodents: Immunocyto- chemistry. J Histochem Cytochem 33:564–568
Strum JM, Singh G, Katyal SL, McDowell EM (1990) Immunochemical localization of Clara cell protein by light and electron microscopy in conducting airways of fetal and neonatal hamster lung. Anat Record 227:77–86
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Emura, M. et al. (1992). New Cell Culture System for Pulmonary Carcinogenesis and Inhalation Toxicology. In: Seemayer, N.H., Hadnagy, W. (eds) Environmental Hygiene III. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77112-5_15
Download citation
DOI: https://doi.org/10.1007/978-3-642-77112-5_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-77114-9
Online ISBN: 978-3-642-77112-5
eBook Packages: Springer Book Archive