New Cell Culture System for Pulmonary Carcinogenesis and Inhalation Toxicology
Relatively little is known about the histogenesis and the underlying mechanisms of lung cancer; in particular about the target cell type(s) of carcinogens (Schüller 1987). The same can be said about the mechanisms of epithelial regeneration in the lung after injury by toxic chemicals. One of the morphologically appreciable responses of the lung to such injury is a hyperplastic growth of bronchiolo-alveolar epithelium. There is speculation that type II pneumocytes or Clara cells may give rise to the hyperplasia. Neuroendocrine (APUD) cells and type I pneumocytes are supposed to be less capable of initiating hyperplasia because of their low activity of cell division, although hyperplasia of the former cell type is experimentally inducible (Schuller et al. 1988). Mutual transformation has even been postulated between type II pneumocytes and Clara cells in certain cases (Schüller 1987). There is another cell type which, occurring in a fetal as well as regenerating adult airway epithelium (McDowell et al. 1984; Emura 1985; Strum et al. 1990), closely resembles the small mucus granule cells (SMGC) postulated to be one of the carcinogen targets in the lung (McDowell and Trump 1983). In our cell culture study (Emura et al. 1990) a large population of cells possessing SMGC characteristics are inducible in vitro from an undifferentiated population of airway epithelial cells.
KeywordsHigh Performance Liquid Chromatography Airway Epithelial Cell Clara Cell Target Cell Type Hyperplastic Growth
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- Brockmeyer C, Emura M, Ochiai A, Riebe M, Mohr U, Hilfrich J (1990) Secretory differentiation of human fetal bronchial epithelial cells in culture. A study by histochemistry and electron microscopy. Exp Pathol 39:11–17Google Scholar
- Emura M (1985) Development of Syrian golden hamster tracheal epithelium during prenatal and immediate postnatal stages. In: Jones TC, Mohr U, Hunt RD (eds) Respiratory system. Springer Berlin Heidelberg New York pp 11–27Google Scholar
- Katyal SL, Estes LW, Lombardi B (1977) Method for the isolation of surfactant from homogenates and lavages of lung of adult, newborn, and fetal rats. Lab Invest 36:585–592Google Scholar
- McDowell EM, Trump BF (1983) Histogenesis of preneoplastic and neoplastic lesions in tracheobronchial epithelium. Surv Synth Pathol Res 2:235–279Google Scholar
- Ochiai A, Emura M, Riebe-Imre M, Mohr U, Hilfrich J, Tahara E, Dungworth DL (1991) Secretory differentiation and cell type identification of a human fetal bronchial epithelial cell line (HFBE). Virchows Arch B in pressGoogle Scholar
- Schüller HM (1987) Experimental carcinogenesis in the peripheral lung. In: McDowell EM (ed) Lung carcinomas. Churchill Livingstone, Edinburgh London Melbourne New York pp 243–254Google Scholar