Leukemias pp 95-100 | Cite as

Interleukin-1 and Tumor Necrosis Factor Production in Acute Nonlymphoid Leukemia

  • G. Specchia
  • E. Erroi
  • F. Colotta
  • L. Bersani
  • N. Pansini
  • A. Mantovani
  • V. Liso
Conference paper

Abstract

Interleukin-1 (IL-1) is a cytokine with an important role in host defense against a number of inflammatory and immunologic challenges. Molecular studies have revealed the existence of two distinct but related species of IL-1, designated IL-1α and IL-1β, which act through a common receptor [6] and appear to have very similar if not identical, biological activities [15]. IL-1 induces the release of other cytokines such as IL-2 [23], inteferon [11], granulocyte-macrophage-colony-stimulating factor (GM-CSF) [25] and granulocyte-colony-stimulating factor (G-CSF) [24]. In vascular endothelium GM-CSF is involved in IL-1-induced colony-stimulating activity (CSA) [22]. Although different cell types have the capacity to produce IL-1, cells of the monocyte-macrophage lineage are a major source of IL-1 [19]. Upon appropriate stimulation, mononuclear phagocytes also release tumor necrosis factor (TNF), a monokine unrelated to IL-1 but with several biological activities [4,18]. IL-1 has been shown to have radioprotective activity in mice [17], an effect that may be related to its direct or indirect action on bone marrow precursors. The capacity of IL-1 to affect hemopoietic precursors together with its action on vessel wall hemostatic properties [14] prompted us to investigate the production of IL-1 in acute nonlymphoid leukemia (ANLL). We report that freshly isolated ANLL cells release considerable amounts of IL-1 upon endotoxin stimulation.

Keywords

Formaldehyde Leukemia Agarose Citrate Sedimentation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • G. Specchia
    • 1
  • E. Erroi
  • F. Colotta
  • L. Bersani
  • N. Pansini
  • A. Mantovani
  • V. Liso
  1. 1.Hematology ServiceUniversity of BariBariItaly

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