Interleukin-1 and Tumor Necrosis Factor Production in Acute Nonlymphoid Leukemia
Interleukin-1 (IL-1) is a cytokine with an important role in host defense against a number of inflammatory and immunologic challenges. Molecular studies have revealed the existence of two distinct but related species of IL-1, designated IL-1α and IL-1β, which act through a common receptor  and appear to have very similar if not identical, biological activities . IL-1 induces the release of other cytokines such as IL-2 , inteferon , granulocyte-macrophage-colony-stimulating factor (GM-CSF)  and granulocyte-colony-stimulating factor (G-CSF) . In vascular endothelium GM-CSF is involved in IL-1-induced colony-stimulating activity (CSA) . Although different cell types have the capacity to produce IL-1, cells of the monocyte-macrophage lineage are a major source of IL-1 . Upon appropriate stimulation, mononuclear phagocytes also release tumor necrosis factor (TNF), a monokine unrelated to IL-1 but with several biological activities [4,18]. IL-1 has been shown to have radioprotective activity in mice , an effect that may be related to its direct or indirect action on bone marrow precursors. The capacity of IL-1 to affect hemopoietic precursors together with its action on vessel wall hemostatic properties  prompted us to investigate the production of IL-1 in acute nonlymphoid leukemia (ANLL). We report that freshly isolated ANLL cells release considerable amounts of IL-1 upon endotoxin stimulation.
KeywordsFormaldehyde Leukemia Agarose Citrate Sedimentation
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