Structure and Function of Antigen Receptor Complexes on Murine B Lymphocytes

  • D. Haustein

Abstract

B lymphocytes express immunoglobulin M (IgM) and D (IgD) on their surface that function as antigen receptors. When both Ig isotypes exist on one individual cell they possess the same antigen specificity. The binding of antigen to the receptors leads, together with other signals, to the activation of the cells. However, the mechanisms and structures by which Ig transmits such signals to the interior of the cells are unknown. One model of an antigen-recognition complex on the lymphocyte surface (Fig. 1) which is involved in the specific triggering of lymphocytes was postulated by us and others many years ago [5, 6]. By analogy with certain polypeptide hormones, this complex could consist of a recognition, a regulator and an effector element. Binding of antigen to the Ig molecule would induce its interaction with the regulator-effector complex which would then trigger a cascade of reactions that finally lead to the activation of the B cell. Since such receptor complexes have not yet been described, the aim of this work was to identify and biochemically characterize molecules which are associated and/or covalently linked to Ig. Furthermore, we are interested in the question whether IgM and IgD are linked to identical or different molecules.

Keywords

Hydrolysis Ethyl Albumin Propionate Phenyl 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Buck LB, Yuan D, Vitetta ES (1979) A dichotomy between the expression of IgD on B cells and its requirement for triggering such cells with two T-independent antigens. J Exp Med 149:987CrossRefGoogle Scholar
  2. 2.
    Coggeshall KM, Cambier JC (1984) B cell activation. VIII. Membrane immunoglobulins transduce signals via activation of phosphatidylinositol hydrolysis. J Immunol 133:3384Google Scholar
  3. 3.
    Ji T (1983) Bifunctional reagents. Meth Enzymol 91:580CrossRefGoogle Scholar
  4. 4.
    Kettmann JR, Cambier JC, Uhr JW, Ligler FS, Vitetta ES (1979) The role of receptor IgM and IgD in determining triggering and induction of tolerance in murine B cells. Immunol Rev 43:69CrossRefGoogle Scholar
  5. 5.
    Koch N, Haustein D (1983) Association of surface IgM with two membrane proteins on murine B lymphocytes detected by chemical crosslinking. Mol Immunol 20:33CrossRefGoogle Scholar
  6. 6.
    Marchalonis JJ (1976) Surface immunoglobulins of B and T lymphocytes: molecular properties, association with the cell membrane, and a unified model of antigen recognition. Contemp Top Mol Immunol 5:125Google Scholar
  7. 7.
    Monroe JG, Cambier JC (1983) B cell activation. I. Anti-immunoglobulin-induced receptor cross-linking results in a decrease in the plasma membrane potential of murine B lymphocytes. J Exp Med 157:2073CrossRefGoogle Scholar
  8. 8.
    Sieckmann DG, Asofsky R, Mosier DE, Zitron IM, Paul WE (1978) Activation of mouse lymphocytes by anti-immunoglobulin. I. Parameters of the proliferative response. J Exp Med 147:814CrossRefGoogle Scholar
  9. 9.
    Vitetta ES, Uhr JW (1977) IgD and B cell differentiation. Immunol Rev 37:50-88CrossRefGoogle Scholar
  10. 10.
    Vogel L, Haustein D (1989) Immunoglobulin subunits of murine B lymphocytes: structure and association with other membrane proteins. Immunology 67:251Google Scholar
  11. 11.
    Weiner HL, Moorhead JW, Claman H (1976) Anti-immunoglobulin stimulation of murine lymphocytes. I. Age dependency of the proliferative response. J Immunol 116:1656Google Scholar
  12. 12.
    Zitron IM, Clevinger BL (1980) Regulation of murine B cells through surface immunoglobulin. I. Monoclonal anti-0 antibody that induces allotype-specific proliferation. J Exp Med 152:1135–1146CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • D. Haustein
    • 1
  1. 1.Paul-Ehrlich-InstitutLangenGermany

Personalised recommendations