Abstract
For a better understanding of the oxygen-sensing mechanism(s) that control erythropoietin (Epo) production, further insights into the regulation of Epo gene expression are necessary. In the hepatoma cell lines HepG2 and Hep3B, Epo is produced in response to the main physiological stimulus, hypoxia (Goldberg et al. 1987). The ability to accurately quantitate the levels of Epo mRNA in these hepatoma cells should provide useful new information about regulation of Epo gene expression. Conventional methods of mRNA analysis such as Northern blot and “dot blot” hybridization and even nuclease protection permit only crude quantitation of mRNA. The sensitivity of these methods is often too low to detect Epo mRNA in uninduced cells or in a small number of cells.
This research was supported by a grant from the Deutsche Forschungsgemeinschaft (Fa 225/1-1).
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© 1992 Springer-Verlag Berlin Heidelberg
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Fandrey, J.K., Bunn, H.F. (1992). Analysis of Erythropoietin Gene Expression by Means of Competitive Polymerase Chain Reaction. In: Pagel, H., Weiss, C., Jelkmann, W. (eds) Pathophysiology and Pharmacology of Erythropoietin. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77074-6_7
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DOI: https://doi.org/10.1007/978-3-642-77074-6_7
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