A Sensitive ELISA for Erythropoietin and Its Application in Various Diseases

  • G. Noé
  • I. Rich
  • B. Kubanek
Conference paper


Radioimmunoassays (RIA) (Sherwood and Goldwasser 1979; Cotes 1982; Egrie et al. 1987) have shown many advantages concerning specificity, accuracy, speed, and sensitivity over in vivo and in vitro bioassays (Cotes and Bangham 1961; Goldwasser et al. 1975; Rich and Kubanek 1976; Krystal 1983). We decided to develop another immunological assay, namely an enzyme-linked immunosorbent assay (ELISA), which has a primary advantage over RIAs in the avoidance of radioactivity. All aspects of the ELISA have been established, so that we can present a well-functioning assay with which it is possible to reliably measure the serum erythropoietin (EPO) concentration in various diseases.


Chronic Myelogenous Leukemia Polycythemia Vera Hairy Cell Leukemia Chronic Leukemia Renal Anemia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Cotes PM (1982) Immunoreactive erythropoietin in serum. I. Evidence for the validity of the assay method and the physiological relevance of estimates. Br J Haematol 50: 427–438PubMedCrossRefGoogle Scholar
  2. Cotes PM, Bangham DR (1961) Bio-assay of erythropoietin in mice made polycythemic by exposure to air at a reduced pressure. Nature 191: 1065–1067PubMedCrossRefGoogle Scholar
  3. Egrie JC, Cotes PM, Lane J, Gaines Das RE, Tam RC (1987) Development of radioimmunoassay for human erythropoietin using recombinant erythropoietin as tracer and immunogen. J Immunol Methods 99: 235–241PubMedCrossRefGoogle Scholar
  4. Goldwasser E, Eliason JF, Sikkema D (1975) Assay for erythropoietin in vitro at the milliunit level. Endocrinology 97: 315–323PubMedCrossRefGoogle Scholar
  5. Jelkmann W, Wiedemann G (1990) Serum erythropoietin level: relationships to blood hemoglobin concentration and erythrocytic activity of the bone marrow. Klin Wochenschr 68: 403–407PubMedCrossRefGoogle Scholar
  6. Krystal G (1983) A simple microassay for erythropoietin based on 3H-thymidine incorporation into spleen cells from phenylhydrazine treated mice. Exp Hematol 11: 649–660PubMedGoogle Scholar
  7. Miller C, Jones RJ, Piantadosi S, Abeloff MD, Spival J (1990) Decreased erythropoietin response in patients with anemia of cancer. N Engl J Med 322: 1689–1692PubMedCrossRefGoogle Scholar
  8. Rhyner K, Egli F, Niemoller M,Wieczorek A, Greminger P,Vetter W (1989) Serumerythropoetinwerte bei verschiedenen Krankheitszustanden. Nephron 51 [Suppl 1]: 39–46PubMedCrossRefGoogle Scholar
  9. Rich IN, Kubanek B (1976) Erythroid colony formation in foetal liver and adult bone marrow and spleen from mouse. Blut 33: 171–180PubMedCrossRefGoogle Scholar
  10. Sherwood JB, Goldwasser E (1979) A radioimmunoassay for erythropoietin. Blood 54: 885–893PubMedGoogle Scholar
  11. Wide L, Bengtsson C, Birgegard G (1989) Circadian rhythm of erythropoietin in human serum. Br J Haematol 72: 85–90PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • G. Noé
  • I. Rich
  • B. Kubanek

There are no affiliations available

Personalised recommendations