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A Sensitive ELISA for Erythropoietin and Its Application in Various Diseases

  • G. Noé
  • I. Rich
  • B. Kubanek
Conference paper

Abstract

Radioimmunoassays (RIA) (Sherwood and Goldwasser 1979; Cotes 1982; Egrie et al. 1987) have shown many advantages concerning specificity, accuracy, speed, and sensitivity over in vivo and in vitro bioassays (Cotes and Bangham 1961; Goldwasser et al. 1975; Rich and Kubanek 1976; Krystal 1983). We decided to develop another immunological assay, namely an enzyme-linked immunosorbent assay (ELISA), which has a primary advantage over RIAs in the avoidance of radioactivity. All aspects of the ELISA have been established, so that we can present a well-functioning assay with which it is possible to reliably measure the serum erythropoietin (EPO) concentration in various diseases.

Keywords

Chronic Myelogenous Leukemia Polycythemia Vera Hairy Cell Leukemia Chronic Leukemia Renal Anemia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • G. Noé
  • I. Rich
  • B. Kubanek

There are no affiliations available

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