Development pp 409-419 | Cite as

The Use of in Situ Hybridisation to Study the Molecular Genetics of Mouse Development

  • David G. Wilkinson


The study of mammalian development is a challenging problem, as even the most amenable system, the mouse, has a number of technical limitations, in particular the difficulty of systematic developmental genetics and of microsurgical manipulations of the embryo. Nevertheless, significant advances towards understanding molecular mechanisms of mouse development have been made through the cloning of many genes with potential roles in embryogenesis. An important step in the analysis of these genes is to determine the spatial and temporal regulation of their expression during development. When combined with other lines of evidence, expression patterns can provide preliminary clues to the developmental function of genes and can also provide insight into mechanisms of development. These lines of evidence include homologies with previously characterised genes (see Lobe and Gruss, Chap. 26, this Vol.), analogies with other developmental systems and the study of mutants. In addition, knowledge of expression patterns is essential for the design and interpretation of reverse genetic manipulations in which genes are inactivated by homologous recombination or misregulated in transgenic mice.


Homeobox Gene Primitive Streak Mouse Development Analyse Expression Pattern Early Drosophila Embryo 
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© Springer-Verlag Berlin Heidelberg 1992

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  • David G. Wilkinson

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