Protein-DNA Interaction at Yeast Replication Origins: An ARS Consensus Binding Protein

  • J. F. X. Hofmann
  • M. Cockell
  • S. M. Gasser
Conference paper
Part of the Colloquium der Gesellschaft für Biologische Chemie book series (MOSBACH, volume 43)

Abstract

Eukaryotic DNA replication initiates at many sites on each chromosome during the S phase of the cell cycle. The identification of yeast chromosomal DNA sequences that act in cis to allow the extrachromosomal maintenance of plasmids (ARS or autonomously replicating sequences, Stinehcomb et al. 1976) in yeast led to the suggestion that they function as origins of replication. Proof that initiation of DNA synthesis occurs at or near ARS elements comes from two-dimensional gel electrophoresis systems (Brewer and Fangman 1987; Huberman et al. 1987) that allow the separation of replication intermediates and the subsequent mapping of initiation sites for DNA replication.

Keywords

Adenosine Electrophoresis Polypeptide Polyacrylamide Thymidine 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • J. F. X. Hofmann
    • 1
  • M. Cockell
    • 1
  • S. M. Gasser
    • 1
  1. 1.Swiss Institute for Experimental Cancer Research (ISREC)Epalinges/LausanneSwitzerland

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