Protein-DNA Interaction at Yeast Replication Origins: An ARS Consensus Binding Protein
Eukaryotic DNA replication initiates at many sites on each chromosome during the S phase of the cell cycle. The identification of yeast chromosomal DNA sequences that act in cis to allow the extrachromosomal maintenance of plasmids (ARS or autonomously replicating sequences, Stinehcomb et al. 1976) in yeast led to the suggestion that they function as origins of replication. Proof that initiation of DNA synthesis occurs at or near ARS elements comes from two-dimensional gel electrophoresis systems (Brewer and Fangman 1987; Huberman et al. 1987) that allow the separation of replication intermediates and the subsequent mapping of initiation sites for DNA replication.
KeywordsAutonomously Replicate Sequence Autonomous Replication Bottom Strand Core Consensus Nuclear Scaffold
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