Protein-DNA Interaction at Yeast Replication Origins: An ARS Consensus Binding Protein
Eukaryotic DNA replication initiates at many sites on each chromosome during the S phase of the cell cycle. The identification of yeast chromosomal DNA sequences that act in cis to allow the extrachromosomal maintenance of plasmids (ARS or autonomously replicating sequences, Stinehcomb et al. 1976) in yeast led to the suggestion that they function as origins of replication. Proof that initiation of DNA synthesis occurs at or near ARS elements comes from two-dimensional gel electrophoresis systems (Brewer and Fangman 1987; Huberman et al. 1987) that allow the separation of replication intermediates and the subsequent mapping of initiation sites for DNA replication.
KeywordsAdenosine Electrophoresis Polypeptide Polyacrylamide Thymidine
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- Hofmann JFX (1991) Components of the yeat nuclear scaffold: RAP-1 and an ARS consensus binding protein. PhD. Thesis, University of Lausanne, Lausanne, Switzerland.Google Scholar
- Umek RM, Linskens MHK, Kowalski D & Huberman JA (1989) New beginnings of eukaryotic DNA replication origins. Biochim et Biophys Acta 1007:1–14Google Scholar