Regulation of the Mitotic CDC2 Protein Kinase
The eukaryotic cell cycle is characterized by two major events, DNA replication (S phase) and mitosis (M phase); these are separated by two so-called “gap”-phases, G1 (prior to S phase) and G2 (prior to M phase). According to the current paradigm of the cell cycle as a “cdc2 cycle”, both DNA replication and mitosis are driven by serine-threonine specific protein kinases encoded by functional homologs of the fission yeast cdc2 gene (for review see Nurse 1990; Pines and Hunter 1990; Draetta 1990). In yeasts, a single 34 kD catalytic subunit (called cdc2 in fission yeast, CDC28 in budding yeast) is required prior to the onset of DNA replication for traversing a control point called Start, as well as for entry into mitosis. In vertebrates, the mitotic function of p34cdc2 is well established, but recent evidence indicates that progression through interphase cell cycle transitions requires the activity of cdc2-related kinases.
KeywordsTyrosine Serine Polypeptide Arginine Beach
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