Abstract
Solanum dulcamara L. (= Dulcamara flexuosa Moench) (Fig. 1), known as dogwood or bittersweet (Solanaceae), is a clambering or prostrate, perennial shrub which may grow to a height of 2 m (Hegi 1927). Its stem is angular and woody with the exception of the herbaceous top and ranges in diameter between 0.25 and 2 cm, rarely up to 5–6 cm. The leaves are alternate, long-stalked, sparsely pubescent on both sides, and quite variable in shape. The oval- to egg-shaped leaf blade is pointed at the tip. Its base, however, may also be cordate, arrow-shaped, or may consist of one or two lobes. Different leaf forms may be found on the same plant. The flowers emerge axillary in panicle-like loose clusters. The calyx bears five narrow teeth; the five joint petals are bright purple and their tips are somewhat reflexed when fully expanded. The five stamens have yellow anthers which form a conspicuous column. The fruit is a round- to egg-shaped berry, green when young and becoming bright red when mature. In Europe, the flowering season is May to September. It is distributed throughout Europe and is also a native to North Africa, West Asia, India, the USSR, China, and Japan. It is not clear whether its occurrence in North America is of native origin or whether it was introduced from Europe. Growth habitats of the plant are wet meadows, ditches, and poplar-alder forests, but it may also grow on dry and sandy soils, waste areas, between gravel or rubble, and in clefts of old walls. The variability in morphological appearance, habitat, and its wide distribution gave rise to the description of several subspecies and varieties. Their taxonomic significance and position, however, is not quite clear and is further complicated by the occurrence of chemovarieties.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ambasta SSP (1986) The useful plants of India. Publication and Information Directorate, CSIR, New Delhi
Bahr V, Hänsel R (1982) Immunomodulation properties of 5,20a(R)-dihydroxy-6a,7a-epoxy-l-oxo- (a5a)-witha-2,24-dienolide and solasodine. Planta Med 44: 32–33
Bhatt PN, Bhatt DP (1984) Regulation of sterol biosynthesis in Solanum species. J Exp Bot 35: 890–896
Bhatt PN, Bhatt DP, Sussex IM (1979) Organ regeneration from leaf disks of Solanum nigrum, S. dulcamara and S. khasianum. Z Pflanzenphysiol 95: 35–362
Bhatt DP, Bhatt PN, Mehta AR (1986) Steroid analysis and plant regeneration from cell suspension cultures of Solanum dulcamara L. Beitr Biol Pflanz 61: 203–213
Binding H, Nehls R (1977) Regeneration of isolated protoplasts to plants in Solanum dulcamara L.. Z Pflanzenphysiol 85: 279–280
Cham BE, Wilson L (1987) HPLC of glycoalkaloids from Solanum sodomaeum. Planta Med, pp 59–62
Cham BE, Gilliver M, Wilson L (1987) Antitumour effects of glycoalkaloids isolated from Solanum sodomaeum. Planta Med, pp 34–36
Chand PK, Ochatt SJ, Rech EL, Power JB, Davey MR (1988) Electroporation stimulates plant regeneration from protoplasts of the woody medicinal speciesSolanum dulcamara L. J Exp Bot 39: 1267–1274
Chand PK, Rech EL, Golds TJ, Power JB, Davey MR (1989) Electroporation stimulates transformation of freshly isolated cell suspension protoplasts of Solanum dulcamara byAgrobacterium. Plant Cell Rep 8: 86–89
Coxon DT, Price KR, Jones PG (1979) A simplified method for the determination of total glycoalkaloids in potato tubers. J Sci Food Agric 30: 1043–1049
Crabbe PG, Fryer C (1980) Rapid quantitative analysis of solasodine, solasodine glycosides and solasodiene by high-pressure liquid chromatography. J Chromatogr 187: 87–100
Crabbe PG, Fryer C (1982) Evaluation of chemical analysis for the determination of solasodine in Solanum laciniatum. J Pharm Sci 71: 1356–1362
Duke JA, Ayensu ES (1985) Medicinal plants of China, vol 2. Reference Publ Inc, Algonac, p 611
Ehmke A, Eilert U (1986) Steroidal alkaloids in tissue cultures and regenerated plants of Solanum dulcamara. Plant Cell Rep 5: 31–34
Ehmke A, Schiebel H-M, McDowell M (1987) Liquid chromatography mass spectrometry. On-line and off-line coupling for the analysis of Solanum glycoalkaloids. Pharmaceut Weekbl Sci Ed 9: 232 (Abstr)
Elridge AC, Hockridge ME (1983) High-performance liquid chromatographic separation of eastern black nightshade (Solanum ptycanthum) glycoalkaloids. J Agric Food Chem 31: 1218–1220
Gaffield W, Keeler RF (1984) Structure and stereochemistry of steroidal amine teratogens. Adv Exp Med Biol 177: 214
Hegi G (1927) Illustrierte Flora von Mitteleuropa V (4). JF Lehmanns Verlag, München, pp 2589–2592
Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 228: 1229–1231
Hsu HY (1986) Oriental materia medica, a concise guide. Oriental Healing Arts Inst, Long Beach, p 437
Hunter IR, Heftmann E (1983) Isolation of glycoalkaloids with the chromatotron and their determination by high-performance liquid chromatography. J Liquid Chromatogr 6: 281–289
Hunter IR, Waiden MK, Wagner J, Heftmann E (1976) Thin-layer chromatography of steroidal alkaloids. J Chromatogr 118: 259–262
Hunter IR, Waiden MK, Heftmann E (1980) High-performance liquid chromatography of Solanum and Veratrum alkaloids. J Chromatogr 198: 363–366
Keeler RF (1975) Toxins and teratogens of higher plants. J Nat Prod 38: 56–86
Keeler RF, Young S, Brown D (1976) Spina bifida, exencephaly, and cranial bleb produced in hamsters by the Solanum alkaloid solasodine. Res Commun Chem Pathol Pharmacol 13: 723–730
Kupchan SM, Barboutis SJ, Know JR, Lau Cam CA (1965) Beta-solamarine: tumor inhibitor isolated fromSolanum dulcamara. Science 150: 1827–1828
Kusano G, Takahashi A, Sugiyama K, Nozoe S (1987a) Antifungal properties of Solanum alkaloids. Chem Pharm Bull 35: 4862–4867Solanum dulcamara L. (Bittersweet) 351
Kusano G, Takahashi A, Nozoe S, Sonoda Y, Sato Y (1987b)Solanum alkaloids as inhibitors of enzymatic conversion of dihydrolanosterol into cholesterol. Chem Pharm Bull 35: 4321–4323
List P, Hörhammer L (1979) Hägers Handbuch der pharmazeutischen Praxis, VI b. Springer, Berlin Heidelberg New York, pp 437–439
Macek TE (1989) Solanum aviculare Forst., Solanum laciniatum Ait (poroporo): in vitro culture and the production of solasodine. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 7. Medicinal and aromatic plants II. Springer, Berlin Heidelberg New York Tokyo, pp 443–467
Mathe I Jr, Mathe I (1978) Steroids ofSolanum dulcamara L. and their variability. Herba Hung 1: 39–55
Mathe I Jr, Mathe I (1979) Variation in alkaloids in Solanum dulcamara L. In: Hawkes JG, Lester RN, Skelding AD (eds) The biology and taxonomy of the solanaceae. Acad Press, London, pp 211–222
Mathe I Jr, Mathe I, Botz L, Koch L (1986) Possibilities of Solanum alkaloid production in european temperate zone. Proc 5th Int Symp Medicinal, aromatic and spice plants. Mungpp/Daijeeling. Acta Hortic 188: 193–201
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Pandeya SC, Saratbabu GV, Bhatt AB (1981) A quick method for the estimation of solasodine in leaves and berries of Solanum sysimbrifolium Lamk. Indian J Exp Biol 19: 1207–1208
Price KR, Mellon FA, Self R, Fenwick GR, Osman SF (1985): Fast atom bombardment mass spectrometry of Solanum glyeoalkaloids and its potential for mixture analysis. Biomed Mass Spectrom 12: 79–85
Roddick JG (1987) Antifungal activity of plant steroids. In: ACS Symp Ser 325. Ecology and metabolism of plant lipids, pp 286–303
Roddick JG (1989) The acetylcholinesterase-inhibitory activity of steroidal glyeoalkaloids and their aglycones. Phytochemistry 28: 2631–2643
Roddick JG, Rijnenberg AL (1986) Effect of steroidal glyeoalkaloids of potato on the permeability of liposome membranes. Physiol Plant 68: 436–440
Roddick JG, Rijnenberg AL, Weissenberg M (1990) Membrane-disrupting properties of the steroidal glyeoalkaloids solasonine and solamargine. Phytochemistry 29: 1513–1518
Rönsch H, Schreiber K (1966)Solanum Alkaloide LXV. Über vier neue 15a-Hydroxy-spirosolan- Alkaloide aus Wurzeln von Solanum dulcamara L.. Liebigs Ann Chem 694: 169–182
Rönsch H, Schreiber K (1967) LXXXIII. Analytische und präparative dünnschichtchromatographische Trennung von 5a-gesättigten bzw. A5-ungesättigten Steroidalkaloiden und -sapogeninen an Ag N03- haltigen Adsorptionsschichten. J Chromatogr 30: 149–154
Sander H (1963) Chemische Differenzierung innerhalb der Art Solanum dulcamara L. Planta Med 11:303–316
Sander H, Willuhn G (1961) ÜberSolanum dulcamara L. 5. Mitt. Isolierung von Tigogenin neben Soladulcidin aus reproduktiven Organen. Flora 151: 150–154
Spencer J, Coutler MF, Coutler P (1982) Topical salve for skin growth removal. Brit UK Pat Appl GB 2,0066,066
Thorne HV, Clarke GF, Suce R (1985) The inactivation of herpes simplex virus by some Solanaceae glyeoalkaloids. Antiviral Res 5: 335–343
Ushio R, Murakami K, Sogawa K (1988) Antitumor extracts (steroidal saponins) of Solanum lyratum. Jpn Kokai Tokkyo Koho JP Chem Abstr 63,202,357
Van Gelder WMG (1985) Determination of the total C-27-steroidal alkaloid composition of Solanum species by high-resolution gas chromatography. J Chromatogr 331: 285–293
Weiler EW, Krüger H, Zenk MH (1980) Radioimmunoassay for the determination of the steroidal alkaloid solasodine and related compounds in living plants and herbarium specimens. Planta Med 39: 112–124
Wetter LR, Constabel F (1982) Plant tissue culture methods, 2nd edn. NRCC Prairie Regional Laboratory, Saskatoon
Willuhn G (1966) Untersuchungen zur chemischen Differenzierung bei Solanum dulcamara L. I. Genetische Fixierung der unterschiedlichen Steroidalkaloidführung. Planta Med 14: 408–420
Willuhn G (1967) Untersuchungen zur chemischen Differenzierung bei Solanum dulcamara L. II. Der Steroidgehalt in Früchten verschiedener Entwicklungsstadien der Tomatidenol- und Soladulcidin- Sippe. Planta Med 15: 58–73
Willuhn G (1968) Untersuchungen zur chemischen Differenzierung bei Solanum dulcamara L. HI. Der Steroidalkaloidgehalt in den Früchten der Sosasodin-Sippe. Planta Med 16: 462–466
Willuhn G, Kun-Anake A (1970) Untersuchungen zur chemischen Differenzierung bei Solanum dulcamara L. V. Isolierung von Tomatidin aus den Wurzeln der Solasodin-Sippe. Planta Med 18: 354–360
Willuhn G, May S (1982) Triterpene und Steroide in Kalluskulturen von Solanum dulcamara. Planta Med 46: 153–158
Wolters B (1964a) Antibiotische Wirkung von Solanum dulcamara gegen Pilze. Naturwissenschaften 51: 111–112
Wolters B (1964b) Beziehungen zwischen Struktur und antibiotischer Wirkung bei einigen Steroidal-kaloiden. Arch Pharm 297: 748–754
Wolters B (1965a) Die antibiotische Wirkung von Solanum dulcamara L. Pharm Z 110: 1718–1720
Wolters B (1965b) Der Anteil der Steroidsaponine an der antibiotischen Wirkung von Solanum dulcamara. Planta Med 18: 189–193
Zenkteler M (1972) In vitro formation of plants from leaves of several species of the Solanaceae family. Biochem Physiol Pflanz 163: 509–512
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Ehmke, A., Eilert, U. (1993). Solanum dulcamara L. (Bittersweet): Accumulation of Steroidal Alkaloids in the Plant and in Different in Vitro Systems. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants IV. Biotechnology in Agriculture and Forestry, vol 21. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77004-3_23
Download citation
DOI: https://doi.org/10.1007/978-3-642-77004-3_23
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-77006-7
Online ISBN: 978-3-642-77004-3
eBook Packages: Springer Book Archive