The Curious Case of the Novel 2-D Gel Artifact

  • Bonita J. Brewer
Conference paper


The rDNA locus in the yeast Saccharomyces cerevisiae consists of approximately 200 tandem copies of a 9.08 kb repeating unit located on the right arm of chromosome XII (reviewed in Warner, 1989). Within each repeat is a 5S rRNA gene and the 18S, 5.8S and 26S rRNA genes transcribed as a 35S RNA precursor (Figure 1). The 5S gene is transcribed by RNA polymerase III and the 35S RNA is transcribed by RNA polymerase I. The orientation of the repeats on the chromosome results in PolIII moving toward the telomere and PolI moving toward the centromere.


Replication Fork rDNA Locus rDNA Fragment rDNA Repeat Replication Intermediate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brewer, B. J. and Fangman, W. L. (1987) The localization of replication origins on ARS Plasmids in S. cerevisiae. Cell 51, 463–471.PubMedCrossRefGoogle Scholar
  2. Brewer, B. J. and Fangman, W. L. (1988) A replication fork barrier at the 3’ end of yeast ribosomal RNA genes. Cell 55, 637–643.PubMedCrossRefGoogle Scholar
  3. Kouprina, N. Y. and Larionov, V. L. (1983) The study of a rDNA replicator in Saccharomyces. Curr. Genet. 1, 433–438.CrossRefGoogle Scholar
  4. Linskens, M. and Huberman, J. A. (1988) Organization of replication of ribosomal DNA in Saccharomycer cerevisiae. Mol. Cell. Biol. 8, 4927–4935.PubMedGoogle Scholar
  5. Saffer, L. D. and Miller, Jr., O. L. (1986) Electron miceroscopic study of Saccharomyces cerevisiae rDNA chromatin replication. Mol. Cell. Biol. 6, 1148–1157.PubMedGoogle Scholar
  6. Warner, J. (1989) Synthesis of Ribosomes in Saccharomyces cerevisiae. Microbiol. Rev. 53, 256–271.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Bonita J. Brewer
    • 1
  1. 1.Department of GeneticsUniversity of WashingtonSeattleUSA

Personalised recommendations