Skip to main content
SpringerLink
Log in

SV40 DNA Replication with Purified Proteins: Functional Interactions Among the Initiation Proteins

  • Conference paper
DNA Replication: The Regulatory Mechanisms

Abstract

Simian virus 40 (SV40), has proved to be a useful model system for the study of mammalian DNA replication (Challberg and Kelly 1989; Hurwitz et al. 1990; Kelly 1988; Stillman 1989). The SV40 genome is replicated by the host machinery in conjunction with a single viral protein, T antigen. Thus, the basic mechanisms involved in the replication of viral DNA are likely to be important for mammalian DNA replication. Synthesis of the viral genome begins at a well-defined origin and, like chromosomal DNA replication, proceeds in a bidirectional and semidiscontinuous manner. The utility of this relatively simple system is exemplified by the development of a cell-free replication system (Li and Kelly 1984; Li and Kelly 1985) which has allowed the identification of factors implicated in the replication of mammalian DNA. Replication in vitro was demonstrated to possess most of the characteristics of in vivo SV40 DNA replication (Li and Kelly 1984; Li and Kelly 1985; Li et al. 1986; Stillman et al. 1985; Stillman and Gluzman 1985; Wobbe et al. 1985). In particular, initiation depended on the viral protein T antigen, occured at the origin of DNA replication, and proceeded bidirectionally. Furthermore, replication intermediates formed in vitro were identical to those found in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Alberts, B. M. (1987). “Prokaryotic DNA replication mechanisms.” Phil. Trans. R. Soc. Lond. 317(supplement): 395–420.

    Article  CAS  Google Scholar 

  • Arai, K. and A. Kornberg. (1979). “A general priming system employing only dnaB protein and primase for DNA replication.” Proc. Natl. Acad. Sci. USA. 76(9): 4308–4312.

    Article  PubMed  CAS  Google Scholar 

  • Borowiec, J. A. and J. Hurwitz. (1988). “Localized melting and structural changes in the SV40 origin of replication induced by T-antigen.” EMBO J. 7(10): 3149–3158.

    PubMed  CAS  Google Scholar 

  • Braithwaite, A. W., H. Sturzbecher, C. Addison, C. Palmer, K. Rudge and J. R. Jenkins. (1987). “Mouse p53 inhibits SV40 origin-dependent DNA replication.” Nature. 329: 458–460.

    Article  PubMed  CAS  Google Scholar 

  • Cha, T. and B. M. Alberts. (1990). “Effects of the bacteriophage T4 gene 41 and gene 32 proteins on RNA primer synthesis: Coupling of leading-and lagging-strand DNA synthesis at a replication fork.” Biochemistry. 29: 1791–1798.

    Article  PubMed  CAS  Google Scholar 

  • Challberg, M. D. and T. J. Kelly. (1989). “Animal virus DNA replication.” Annu. Rev. Biochem. 58: 671–717.

    Article  PubMed  CAS  Google Scholar 

  • Collins, K. L. and T. J. Kelly. (1991). “Effects of T antigen and replication protein A on the initiation of DNA synthesis by DNA polymerase α-primase.” Mol. Cell. Biol. 11: 1542–1553.

    Google Scholar 

  • Dean, F. B., J. A. Borowiec, Y. Ishimi, S. Deb, P. Tegtmeyer and J. Hurwitz. (1987). “Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.” Proc. Natl. Acad. Sci. USA. 84: 8267–8271.

    Article  PubMed  CAS  Google Scholar 

  • Dean, F. B., M. Dodson, H. Echols and J. Hurwitz. (1987). “ATP-Dependent formation of a specialized nucleoprotein structure by simian virus 40 (SV40) large tumor antigen at the SV40 replication origin.” Proc. Natl. Acad. Sci USA. 84: 8981–8985.

    Article  PubMed  CAS  Google Scholar 

  • Dornreiter, I., A. Hoss, A. K. Arthur and E. Fanning. (1990). “SV40 T antigen binds directly to the large subunit of purified DNA polymerase alpha.” EMBO J. 9(10): 3329–3336.

    PubMed  CAS  Google Scholar 

  • Erdile, L. F., W. Heyer, R. Kolodner and T. J. Kelly. (1991). “Characterization of a cDNA encoding the 70-kDa subunit of human replication protein A (RP-A), a single-stranded DNA-binding protein involved in DNA replication and recombination.” J. Biol. Chem., in press.

    Google Scholar 

  • Gannon, J. V. and D. P. Lane. (1987). “p53 and DNA polymerase alpha compete for binding to SV40 T antigen.” Nature. 329: 456–458.

    Article  PubMed  CAS  Google Scholar 

  • Gannon, J. V. and D. P. Lane. (1990). “Interactions between SV40 T antigen and DNA polymerase a.” New Biol. 2(1): 84–92.

    PubMed  CAS  Google Scholar 

  • Hurwitz, J., F. B. Dean, A. D. Kwong and S. Lee. (1990). “The in vitro replication of DNA containing the SV40 origin.” JBC. 265(30): 18043–18046.

    CAS  Google Scholar 

  • Kelly, T. J. (1988). “SV40 DNA replication.” J. Biol. Chem. 263: 17889–17892.

    PubMed  CAS  Google Scholar 

  • Kenny, M. K., S.-H. Lee, and J. Hurwitz. (1989). “Multiple functions of human single-stranded DNA binding protein in simian virus 40 DNA replication: single strand stabilization and stimulation of DNA polymerases alpha and delta.” Proc. Natl. Acad. Sci. USA. 86: 9757–9761.

    Article  PubMed  CAS  Google Scholar 

  • Kornberg, A. (1980). DNA Replication. San Francisco,CA, Freeman Publications.

    Google Scholar 

  • LeBowitz J. H. and R. McMacken. (1986). “The Escherichia coli dnaB replication protein is a DNA helicase.” J. Biol. Chem. 261: 4738–4748.

    PubMed  CAS  Google Scholar 

  • Lee, S.-H., T. Eki and J. Hurwitz. (1989). “Synthesis of DNA containing the simian virus 40 origin of replication by the combined action of DNA polymerases alpha and delta.” Proc. Natl. Acad. Sci. USA. 86(19): 7361–7365.

    Article  PubMed  CAS  Google Scholar 

  • Li, J. J. and T. J. Kelly. (1984). “Simian virus 40 DNA replication in vitro.” Proc. Natl. Acad. Sci. USA. 81: 6973–6977.

    Article  PubMed  CAS  Google Scholar 

  • Li, J. J. and T. J. Kelly. (1985). “Simian virus 40 DNA replication in vitro: Specificity of initiation and evidence for bidirectional replication.” Mol. Cell. Biol. 5: 1238–1246.

    PubMed  CAS  Google Scholar 

  • Li, J. J., K. W. C. Peden, R. A. F. Dixon and T. J. Kelly. (1986). “Functional organization of the simian virus 40 origin of DNA replication.” Mol. Cell. Biol. 6: 1117–1128.

    PubMed  CAS  Google Scholar 

  • Mastrangelo, I. A., P. V. Hough, J. S. Wall, M. Dodson, F. B. Dean and J. Hurwitz. (1989). “ATP-dependent assembly of double hexamers of SV40 T antigen at the viral origin of DNA replication.” Nature. 338(6217): 658–662.

    Article  PubMed  CAS  Google Scholar 

  • Matsumoto, T., T. Eki and J. Hurwitz. (1990). “Studies on the initiation and elongation reactions in the simian virus 40 DNA replication system.” Proc. Natl. Acad. Sci. 87: 9712–9716.

    Article  PubMed  CAS  Google Scholar 

  • Murakami, Y., C. R. Wobbe, L. Weissbach, F. B. Dean and J. Hurwitz. (1986). “Role of DNA polymerase a and DNA primase in simian virus 40 DNA replication in vitro.” Proc. Natl. Acad. Sci. USA. 83: 2869–2873.

    Article  PubMed  CAS  Google Scholar 

  • Prelich, G., M. Kostura, D. R. Marshak, M. B. Mathews and B. Stillman. (1987). “The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro” Nature. 326(6112): 471–475.

    Article  PubMed  CAS  Google Scholar 

  • Prelich, G., C. K. Tan, M. Kostura, M. B. Mathews, A. G. So, K. M. Downey and B. Stillman. (1987). “Functional identity of proliferating cell nuclear antigen and a DNA polymerase-delta auxiliary protein.” Nature. 326(6112): 517–520.

    Article  PubMed  CAS  Google Scholar 

  • Richardson, C. C. (1983). “Bacteriophage T7: minimal requirements for the replication of a duplex DNA molecule.” Cell. 33: 315–317.

    Article  PubMed  CAS  Google Scholar 

  • Richardson, R. W. and N. G. Nossal. (1989). “Trypsin cleavage in the COOH terminus of the bacteriophage T4 gene 41 DNA helicase alters the primase-helicase activities of the T4 replication complex in vitro.” J. Biol. Chem. 264(8): 4732–4739.

    PubMed  CAS  Google Scholar 

  • Smale, S. T. and R. Tjian. (1986). “T-antigen-DNA polymerase alpha complex implicated in simian virus 40 DNA replication.” Mol. Cell. Biol. 6(11): 4077–4087.

    PubMed  CAS  Google Scholar 

  • Stahl, H., P. Droge and R. Knippers. (1986). “DNA helicase activity of SV40 large tumor antigen.” EMBO J. 5: 1939–1944.

    PubMed  CAS  Google Scholar 

  • Stillman, B. (1989). “Initiation of eukaryotic DNA replication in vitro” Ann. Rev. Cell Biol. 5: 197–245.

    Article  PubMed  CAS  Google Scholar 

  • Stillman, B. W., R. D. Gerard, R. A. Guggenheimer and Y. Gluzman. (1985). “T antigen and template requirements for SV40 DNA replication in vitro.” EMBO J. 4: 2933–2939.

    PubMed  CAS  Google Scholar 

  • Stillman, B. W. and Y. Gluzman. (1985). “Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.” Mol. Cell. Biol. 5(8): 2051–2060.

    PubMed  CAS  Google Scholar 

  • Tegtmeyer, P., B. Lewton, A. Delucia, V. Wilson and K. Ryder. (1983). “Topography of simian virus 40 A protein-DNA complexes: arrangement of protein bound to the origin of replication.” J. Virol. 46: 151–161.

    PubMed  CAS  Google Scholar 

  • Tsurimoto, T., T. Melendy and B. Stillman. (1990). “Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin.” Nature. 346: 534–539.

    Article  PubMed  CAS  Google Scholar 

  • Tsurimoto, T. and B. Stillman. (1989a). “Multiple replication factors augment DNA synthesis by the two eukaryotic DNA polymerases, alpha and delta.” EMBO J. 8(12): 3883–3889.

    PubMed  CAS  Google Scholar 

  • Tsurimoto, T. and B. Stillman. (1989b). “Purification of a cellular replication factor, RF-C, that is required for coordinated synthesis of leading and lagging strands during simian virus 40 DNA replication in vitro.” Mol. Cell. Biol. 9(2): 609–619.

    PubMed  CAS  Google Scholar 

  • Virshup, D. M., M. G. Kauffman and T. J. Kelly. (1989). “Activation of SV40 DNA replication in vitro by cellular protein phosphatase 2A.” EMBO J. 8(12): 3891–3898.

    PubMed  CAS  Google Scholar 

  • Virshup, D. M. and T. J. Kelly. (1989). “Purification of replication protein C, a cellular protein involved in the initial stages of SV40 DNA replication in vitro.” Proc. Natl. Acad. Sci. USA. 86(10): 3584–3588.

    Article  PubMed  CAS  Google Scholar 

  • Wang, E. H., P. N. Friedman and C. Prives. (1989). “The murine p53 protein blocks replication of SV40 DNA in vitro by inhibiting the initiation functions of SV40 large T antigen.” Cell. 57: 379–392.

    Article  PubMed  CAS  Google Scholar 

  • Weinberg, D. H., K. L. Collins, P. Simancek, A. Russo, M. S. Wold, D. M. Virshup and T. J. Kelly. (1990). “Reconstitution of SV40 DNA replication with purified proteins.” PNAS. 87: 8692–8696.

    Article  PubMed  CAS  Google Scholar 

  • Weinberg, D. H. and T. J. Kelly. (1989). “Requirement for two DNA polymerases in the replication of simian virus 40 DNA in vitro” Proc. Natl. Acad. Sci. USA. 86: 9742–9746.

    Article  PubMed  CAS  Google Scholar 

  • Wiekowski, M., P. Droge and H. Stahl. (1987). “Monoclonal antibodies as probes for a function of large T antigen during the elongation process of simian virus 40 DNA replication.” J. Virol. 61: 411–418.

    PubMed  CAS  Google Scholar 

  • Wobbe, C. R., F. Dean, L. Weissbach and J. Hurwitz. (1985). “In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site.” Proc. Natl. Acad. Sci. USA. 82: 5710–5714.

    Article  PubMed  CAS  Google Scholar 

  • Wobbe, C. R., L. Weissbach, J. A. Borowiec, F. B. Dean, Y. Murakami, P. Bullock and J. Hurwitz. (1987). “Replication of SV40-origin-containing DNA with purified proteins.” Proc. Natl. Acad. Sci., USA. 84: 1834–1838.

    Article  PubMed  CAS  Google Scholar 

  • Wold, M. S., J. J. Li and T. J. Kelly. (1987). “Initiation of simian virus 40 DNA replication in vitro: Large-tumor-antigen- and origin-dependent unwinding of the template.” Proc. Natl. Acad. Sci. USA. 84: 3643–3647.

    Article  PubMed  CAS  Google Scholar 

  • Wold, M. S., D. W. Weinberg, D. M. Virshup, J. J. Li and T. J. Kelly. (1989). “Identification of cellular proteins required for SV40 DNA replication.” J. Biol. Chem. 264: 2801–2809.

    PubMed  CAS  Google Scholar 

  • Yang, L., M. S. Wold, J. J. Li, T. J. Kelly and L. F. Liu. (1987). “Roles of DNA topoisomerases in SV40 DNA replication in vitro” Proc. Natl. Acad. Sci. USA. 84: 950–954.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology and Genetics, School of Medicine, Johns Hopkins University, 21205, Baltimore, Maryland, USA

    Kathleen L. Collins, Lome F. Erdile, Sandra K. Randall, Alicia A. R. Russo, Pamela R. Simancek, Christopher B. Umbricht, David M. Virshup, David H. Weinberg, Marc S. Wold & Thomas J. Kelly

Authors
  1. Kathleen L. Collins
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lome F. Erdile
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sandra K. Randall
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alicia A. R. Russo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Pamela R. Simancek
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Christopher B. Umbricht
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. David M. Virshup
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. David H. Weinberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Marc S. Wold
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Thomas J. Kelly
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut Jacques Monod, CNRS, Université Paris 7, 2 place Jussieu, 75251, Paris Cedex 05, France

    Patrick Hughes  & Masamichi Kohiyama  & 

  2. Institut für Biochemie, Karlstraße 23, 8000, München 2, Germany

    Ellen Fanning

Rights and permissions

Reprints and permissions

Copyright information

© 1992 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Collins, K.L. et al. (1992). SV40 DNA Replication with Purified Proteins: Functional Interactions Among the Initiation Proteins. In: Hughes, P., Fanning, E., Kohiyama, M. (eds) DNA Replication: The Regulatory Mechanisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76988-7_33

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-76988-7_33

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76990-0

  • Online ISBN: 978-3-642-76988-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation