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The POL1 and POL3 DNA Synthesis Genes in Fission Yeast Schizosaccharomyces pombe

  • G. Baldacci
  • D. Bouvier
  • V. Damagnez
  • G. Pignède
  • A-M. de Recondo

Abstract

We isolated and sequenced the S. pombe genes encoding the catalytic subunits of DNA polymerases a (POL1) and δ (POL3) by sequence homology with S. cerevisiae gene probes. Both genes contain a short intron in the 5’ region of the open reading frame. About 300 bp 5’ to POL1 is located the RPL7b gene which encodes a predicted ribosomal protein and is divergently transcribed. The codon usage of POL1 and POL3 is in agreement with both genes being weakly expressed; on the contrary, RPL7b shows a codon usage characteristic of highly expressed genes. The pol α and pol δ predicted peptides were compared with known sequences of DNA polymerases. Both polypeptides contain the seven conserved amino acid blocks diagnostic of a super-family of DNA polymerases. Moreover, pol a shows additional blocks conserved only in α-type eukaryotic DNA polymerases known to date, and pol δ shows a high overall identity percentage with the homologous S. cerevisiae protein indicating an important structural inter-species conservation. We also raised antibodies against two fusion proteins containing E. coli β-galactosidase and part of either pol α or pol δ. These antibodies inhibit in vitro two distinct DNA polymerase activities present in S. pombe cell extracts.

Keywords

Proliferate Cell Nuclear Antigen Codon Usage Catalytic Subunit Fission Yeast POL3 Code 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • G. Baldacci
    • 1
  • D. Bouvier
    • 1
  • V. Damagnez
    • 1
  • G. Pignède
    • 1
  • A-M. de Recondo
    • 1
  1. 1.Laboratoire de Biologie Moléculaire de la RéplicationC.N.R.S. UPR 272VillejuifFrance

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