Abstract
We isolated and sequenced the S. pombe genes encoding the catalytic subunits of DNA polymerases a (POL1) and δ (POL3) by sequence homology with S. cerevisiae gene probes. Both genes contain a short intron in the 5’ region of the open reading frame. About 300 bp 5’ to POL1 is located the RPL7b gene which encodes a predicted ribosomal protein and is divergently transcribed. The codon usage of POL1 and POL3 is in agreement with both genes being weakly expressed; on the contrary, RPL7b shows a codon usage characteristic of highly expressed genes. The pol α and pol δ predicted peptides were compared with known sequences of DNA polymerases. Both polypeptides contain the seven conserved amino acid blocks diagnostic of a super-family of DNA polymerases. Moreover, pol a shows additional blocks conserved only in α-type eukaryotic DNA polymerases known to date, and pol δ shows a high overall identity percentage with the homologous S. cerevisiae protein indicating an important structural inter-species conservation. We also raised antibodies against two fusion proteins containing E. coli β-galactosidase and part of either pol α or pol δ. These antibodies inhibit in vitro two distinct DNA polymerase activities present in S. pombe cell extracts.
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© 1992 Springer-Verlag Berlin Heidelberg
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Baldacci, G., Bouvier, D., Damagnez, V., Pignède, G., de Recondo, AM. (1992). The POL1 and POL3 DNA Synthesis Genes in Fission Yeast Schizosaccharomyces pombe . In: Hughes, P., Fanning, E., Kohiyama, M. (eds) DNA Replication: The Regulatory Mechanisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76988-7_24
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DOI: https://doi.org/10.1007/978-3-642-76988-7_24
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