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Screens for Proteins Binding to the ARS Consensus Sequence

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DNA Replication: The Regulatory Mechanisms

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Abstract

A subset of autonomously replicating sequence (ARS) elements, identified on the basis of their ability to promote high efficiency transformation and extrachromosomal maintenance of plasmids in Saccharomyces cerevisiae, has recently been shown to function as bona fide chromosomal replication origins (Huberman et al. 1988, Dubey et al. 1991, Greenfeder and Newlon, ms. in prep.). Extensive analysis of the DNA sequences required for ARS function on plasmids has revealed the necessity for an eleven base pair (bp) consensus sequence and a variable number of bases 3’ to the T-rich strand of the consensus sequence (reviewed by Newlon 1988, Campbell and Newlon 1991). Mutational analysis of the consensus sequence demonstrated a stringent requirement for most of the bases in the consensus sequence, a finding which suggests that this sequence may function as a protein binding site. In vivo footprinting at ARS1 is consistent with the binding of a protein in the region of the consensus sequence (Lohr and Torchia 1988).

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Author information

Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, 185 South Orange Avenue, 07103, Newark, N.J., USA

    James F. Theis & Carol S. Newlon

Authors
  1. James F. Theis
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  2. Carol S. Newlon
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Editor information

Editors and Affiliations

  1. Institut Jacques Monod, CNRS, Université Paris 7, 2 place Jussieu, 75251, Paris Cedex 05, France

    Patrick Hughes  & Masamichi Kohiyama  & 

  2. Institut für Biochemie, Karlstraße 23, 8000, München 2, Germany

    Ellen Fanning

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© 1992 Springer-Verlag Berlin Heidelberg

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Theis, J.F., Newlon, C.S. (1992). Screens for Proteins Binding to the ARS Consensus Sequence. In: Hughes, P., Fanning, E., Kohiyama, M. (eds) DNA Replication: The Regulatory Mechanisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76988-7_16

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  • DOI: https://doi.org/10.1007/978-3-642-76988-7_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76990-0

  • Online ISBN: 978-3-642-76988-7

  • eBook Packages: Springer Book Archive

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