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DnaA Protein Mediated Transcription Termination in the GUA Operon of Escherichia coli

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Book cover DNA Replication: The Regulatory Mechanisms

Summary

The guaB gene is the first gene in GMP biosynthesis. It harbours a potential DnaA protein binding site (dnaA box, 5’-TTATCCACA) in its coding region. This box is in the active orientation for DnaA-mediated transcription termination. The location of the guaB promoter was defined using a promoter search plasmid with galK as a monitoring gene. Transcription originating at the promoter and entering the gene was quantified under varying levels of DnaA protein. The formation of the DnaA protein/dnaA box complex in the coding region of guaB resulted in a decrease in guaB expression due to transcription termination. DnaA protein, therefore, represents a regulatory element in guaB expression.

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Author notes

  1. Christoph Schaefer

    Present address: Institute of Brain Research, August-Forel-Straße 1, CH-8029, Zürich, Switzerland

Authors and Affiliations

  1. Abt. Trautner, Max-Planck-Institut für molekulare Genetik, Ihnestrasse 63, D-1000, Berlin 33, Germany

    Christoph Schaefer, Andreas Holz & Walter Messer

Authors
  1. Christoph Schaefer
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  2. Andreas Holz
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  3. Walter Messer
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Editor information

Editors and Affiliations

  1. Institut Jacques Monod, CNRS, Université Paris 7, 2 place Jussieu, 75251, Paris Cedex 05, France

    Patrick Hughes  & Masamichi Kohiyama  & 

  2. Institut für Biochemie, Karlstraße 23, 8000, München 2, Germany

    Ellen Fanning

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© 1992 Springer-Verlag Berlin Heidelberg

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Schaefer, C., Holz, A., Messer, W. (1992). DnaA Protein Mediated Transcription Termination in the GUA Operon of Escherichia coli . In: Hughes, P., Fanning, E., Kohiyama, M. (eds) DNA Replication: The Regulatory Mechanisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76988-7_15

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  • DOI: https://doi.org/10.1007/978-3-642-76988-7_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76990-0

  • Online ISBN: 978-3-642-76988-7

  • eBook Packages: Springer Book Archive

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