The Role of DnaA-DnaA Box Interaction in Initiation of Chromosomal Replication in Bacillus subtilis

  • N. Ogasawara
  • S. Moriya
  • H. Yoshikawa
Conference paper

Abstract

In bacteria, an autonomously replicating sequence (ars) was first isolated from the replication origin region of the Escherichia coli chromosome (Yasuda and Hirota, 1977) and was defined as oriC (origin of the chromosomal replication). Genetic and biochemical studies in E. coli, on the other hand, showed that DnaA protein acts at the first step in the initiation process by binding to oriC, and then opening the double strand to make ready for the formation of primosome (von Meyenburg and Hansen, 1987; Bramhill and Komberg, 1988). Thus, oriC and DnaA protein are considered to be the replicator and initiator of the E. coli chromosome. Similar studies in Bacillus subtilis have failed to identify either the ars or a dnaA gene counterpart in this bacterium. Furthermore, a B. subtilis initiator gene dnaB was found to be similar to E. coli dnaA in function but has no structural similarity (Ogasawara et al., 1986). Therefore it was once considered that each bacterium has its unique machinery for the initiation of chromosomal replication and its regulation.

Keywords

Bacillus Oligomer Half Life Pseudomonas Chloramphenicol 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • N. Ogasawara
    • 1
  • S. Moriya
    • 1
  • H. Yoshikawa
    • 1
  1. 1.Departement of GeneticsOsaka University Medical SchoolSuita 565Japan

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