The Role of DnaA-DnaA Box Interaction in Initiation of Chromosomal Replication in Bacillus subtilis

  • N. Ogasawara
  • S. Moriya
  • H. Yoshikawa
Conference paper


In bacteria, an autonomously replicating sequence (ars) was first isolated from the replication origin region of the Escherichia coli chromosome (Yasuda and Hirota, 1977) and was defined as oriC (origin of the chromosomal replication). Genetic and biochemical studies in E. coli, on the other hand, showed that DnaA protein acts at the first step in the initiation process by binding to oriC, and then opening the double strand to make ready for the formation of primosome (von Meyenburg and Hansen, 1987; Bramhill and Komberg, 1988). Thus, oriC and DnaA protein are considered to be the replicator and initiator of the E. coli chromosome. Similar studies in Bacillus subtilis have failed to identify either the ars or a dnaA gene counterpart in this bacterium. Furthermore, a B. subtilis initiator gene dnaB was found to be similar to E. coli dnaA in function but has no structural similarity (Ogasawara et al., 1986). Therefore it was once considered that each bacterium has its unique machinery for the initiation of chromosomal replication and its regulation.


Replication Origin dnaA Gene Origin Region Micrococcus Luteus Chromosomal Replication 
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  1. Bramhill D, Kornberg A (1988) A model for initiation at origins of DNA replication. Cell 54:915–918PubMedCrossRefGoogle Scholar
  2. Brewer BJ (1990) Replication and transcriptional organization of the Escherichia coli chromosome, in The Bacterial Chromosome (Drlica K, Riley M eds) pp61–83 Amer Soc Microbiol Washington DCGoogle Scholar
  3. Fujita MQ, Yoshikawa H, Ogasawara N (1989) Structure of the dnaA region of Pseudomonas putida. Mol Gen Genet 215:381–387PubMedCrossRefGoogle Scholar
  4. Fujita MQ, Yoshikawa H, Ogasawara N (1990) Structure of the dnaA region of Micrococcus luteus: Conservation and variation among eubacteria. Gene 93:73–78PubMedCrossRefGoogle Scholar
  5. Moriya S, Fukuoka T, Ogasawara N, Yoshikawa H (1988) Regulation of initiation of the chromosomal replication by DnaA-boxes in the origin region of the Bacillus subtilis chromosome. EMBO J 7:2911–2917PubMedGoogle Scholar
  6. Moriya S, Kato K, Yoshikawa H, Ogasawara N (1990) Isolation of a dnaA mutant defective in initiation of replication in Bacillus subtilis. EMBO J 9:2805–2910Google Scholar
  7. Nordström K (1990) Control of plasmid replication-how do DNA iterons set the replication frequency? Cell 63:1121–1124PubMedCrossRefGoogle Scholar
  8. Ogasawara N, Fujita MQ, Moriya S, Fukuoka T, Hirano M, Yoshikawa H (1990) Comparative anatomy of oriC of eubacteria. in The Bacterial Chromosome (Drlica K, Riley M eds) pp287–298 Amer Soc Microbiol Washington DC.Google Scholar
  9. Ogasawara N, Moriya S, Mazza PG, Yoshikawa H (1986) Nucleotide sequence and organization of dnaB gene and neighboring genes on the Bacillus subtilis chromosome. Nucleic Acids Res 14:9989–9999PubMedCrossRefGoogle Scholar
  10. Ogasawara N, Moriya S, von Meyenburg K, Hansen F, Yoshikawa H (1985) Conservation of genes and their organization in the chromosomal replication origin of Bacillus subtilis and Escherichia coli. EMBO J 4:3345–3350PubMedGoogle Scholar
  11. Oka A, Sugimoto K, Takanami M, Hirota Y (1980) Replication origin of the Escherichia coli K-12 chromosome. J Mol Biol 176:443–458CrossRefGoogle Scholar
  12. von Meyenburg K, Hansen FG (1987) Regulation of chromosome replication, in E. coli and S. typhimurium: Cellular and Molecular Biology (Neidhardt FC, Ingraham JL, Low KB, Magasanik, B, Schaechter M, Umbarger HE eds) pp1555–1577 Am Soc Microbiol Washington DCGoogle Scholar
  13. Yasuda S, Hirota Y (1977) Cloning and mapping of the replication origin of Escherichia coli. Proc Natl Acad Sci USA 74:5458–5462PubMedCrossRefGoogle Scholar
  14. Yee YW, Smith DW (1990) Pseudomonas chromosomal replication origins: A bacterial class distinct from Escherichia coli type origins. Proc Natl Acad Sci USA 87:1278–1282PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • N. Ogasawara
    • 1
  • S. Moriya
    • 1
  • H. Yoshikawa
    • 1
  1. 1.Departement of GeneticsOsaka University Medical SchoolSuita 565Japan

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