The Role of DnaA-DnaA Box Interaction in Initiation of Chromosomal Replication in Bacillus subtilis
In bacteria, an autonomously replicating sequence (ars) was first isolated from the replication origin region of the Escherichia coli chromosome (Yasuda and Hirota, 1977) and was defined as oriC (origin of the chromosomal replication). Genetic and biochemical studies in E. coli, on the other hand, showed that DnaA protein acts at the first step in the initiation process by binding to oriC, and then opening the double strand to make ready for the formation of primosome (von Meyenburg and Hansen, 1987; Bramhill and Komberg, 1988). Thus, oriC and DnaA protein are considered to be the replicator and initiator of the E. coli chromosome. Similar studies in Bacillus subtilis have failed to identify either the ars or a dnaA gene counterpart in this bacterium. Furthermore, a B. subtilis initiator gene dnaB was found to be similar to E. coli dnaA in function but has no structural similarity (Ogasawara et al., 1986). Therefore it was once considered that each bacterium has its unique machinery for the initiation of chromosomal replication and its regulation.
KeywordsReplication Origin dnaA Gene Origin Region Micrococcus Luteus Chromosomal Replication
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- Brewer BJ (1990) Replication and transcriptional organization of the Escherichia coli chromosome, in The Bacterial Chromosome (Drlica K, Riley M eds) pp61–83 Amer Soc Microbiol Washington DCGoogle Scholar
- Moriya S, Kato K, Yoshikawa H, Ogasawara N (1990) Isolation of a dnaA mutant defective in initiation of replication in Bacillus subtilis. EMBO J 9:2805–2910Google Scholar
- Ogasawara N, Fujita MQ, Moriya S, Fukuoka T, Hirano M, Yoshikawa H (1990) Comparative anatomy of oriC of eubacteria. in The Bacterial Chromosome (Drlica K, Riley M eds) pp287–298 Amer Soc Microbiol Washington DC.Google Scholar
- von Meyenburg K, Hansen FG (1987) Regulation of chromosome replication, in E. coli and S. typhimurium: Cellular and Molecular Biology (Neidhardt FC, Ingraham JL, Low KB, Magasanik, B, Schaechter M, Umbarger HE eds) pp1555–1577 Am Soc Microbiol Washington DCGoogle Scholar