Abstract
A number of mutations that improve the replication competence of plasmids containing defective origins of replication have been isolated inSaccharomyces cerevisiae. Some of these are cis-acting, and generate origins de novo by mutation of prokaryotic vector DNA. Other mutations are trans-acting, and complementation analysis has identified a number of yeast genes whose products may have a role in chromosome replication. One gene thus identified, RAR5, encodes a 175 kDa product, and appears to behave as a repressor of replication origin function. The RAR5 gene is identical to DST2, encoding a strand transferase activity, and KEM1, identified by mutations that enhance the karyogamy defect of a kar\ mutation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Brewer, BJ (1988) Cell 53:679–686.
Brewer, BJ and Fangman, WL (1987) Cell 51:463–471.
Brewer, BJ and Fangman, WL (1988) Cell 55:637–643.
Broach, JR, Li, YY, Feldman, J, Jayaram, M, Abraham, J, Nasmyth, KA and Hicks, JB (1983) Cold Spring Harbor Symp. Quant. Biol. 47:1165–1173.
Diffley, JFX and Stillman, B (1990) Trends in Genetics 6:427–432.
Dykstra, CC, Hamatake, RK and Sugino, A (1990a) J. Biol. Chem. 265: 10968–10973.
Dykstra, CC, Kitada, K, Clark, AB, Hamatake, RK and Sugino, A (1990b) DST2 sequence: EMBL Accession Number M36725.
Hand, R (1978) Cell 15:317–325.
Hieter, P, Mann, C, Snyder, M and Davies, RW (1985) Cell 40:381–392.
Huberman, JA, Zhu, LR, Davis, LR and Newlon, CS (1988) Nucleic Acids Res. 16:6373–6384.
Kearsey, S (1984) Cell 37:299–307.
Kearsey, SE and Edwards, J (1987) Mol. Gen. Genet. 210:509–517.
Kim, J, Ljungdahl, PO and Fink, GR (1990) Genetics 126:799–812.
Kipling, D and Kearsey, SE (1990) Mol. Cell. Biol. 10:265–272.
Kipling, D, Tambini, C and Kearsey, SE (1991) Nucleic Acids Res. (in press).
Koshland, D, Kent, JC and Hartwell, LH (1985) Cell 40:393–403.
Maine, GT, Sinha, P and Tye, B-K (1984) Genetics 106:365–385.
Morrison, A, Araki, H, Clark, AB, Hamatake, RK and Sugino, A (1990) Cell 62:1143–1151.
Newlon, CS (1988) Microbiol. Reviews 52:568–601.
Palzkill, TG and Newlon, CS (1988) Cell 53:441–450.
Reynolds, AE, McCarroll, RM, Newlon, CS, and Fangman, WL (1989) Mol. Cell. Biol. 9: 4488–4494
Rose MD, and Fink, GR (1987) Cell 48:1047–1060.
Snyder, M, Sapolsky, RJ and Davies, RW (1988) Mol. Cell. Biol. 8:2184–2194.
Thrash-Bingham, C and Fangman, WL (1989) Mol. Cell. Biol. 9:809–816.
Umek, RM, Linskens, MHK, Kowalski, D and Huberman, JA (1989) Biochem. Biophys. Acta 1007:1–14.
van Houten, JV and Newlon, CS (1990) Mol. Cell. Biol. 10:3917–3925.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Kearsey, S.E., Kipling, D. (1992). Mutations Affecting Replication Origin Function in Yeast. In: Hughes, P., Fanning, E., Kohiyama, M. (eds) DNA Replication: The Regulatory Mechanisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76988-7_11
Download citation
DOI: https://doi.org/10.1007/978-3-642-76988-7_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-76990-0
Online ISBN: 978-3-642-76988-7
eBook Packages: Springer Book Archive