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Mutations Affecting Replication Origin Function in Yeast

  • Stephen E. Kearsey
  • David Kipling
Conference paper

Abstract

A number of mutations that improve the replication competence of plasmids containing defective origins of replication have been isolated inSaccharomyces cerevisiae. Some of these are cis-acting, and generate origins de novo by mutation of prokaryotic vector DNA. Other mutations are trans-acting, and complementation analysis has identified a number of yeast genes whose products may have a role in chromosome replication. One gene thus identified, RAR5, encodes a 175 kDa product, and appears to behave as a repressor of replication origin function. The RAR5 gene is identical to DST2, encoding a strand transferase activity, and KEM1, identified by mutations that enhance the karyogamy defect of a kar\ mutation.

Keywords

Replication Origin Plasmid Stability RAR5 Gene Mitotic Stability Autonomous Replication 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Stephen E. Kearsey
    • 1
  • David Kipling
    • 1
  1. 1.Department of ZoologyUniversity of OxfordOxfordUK

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