Mutations Affecting Replication Origin Function in Yeast
A number of mutations that improve the replication competence of plasmids containing defective origins of replication have been isolated inSaccharomyces cerevisiae. Some of these are cis-acting, and generate origins de novo by mutation of prokaryotic vector DNA. Other mutations are trans-acting, and complementation analysis has identified a number of yeast genes whose products may have a role in chromosome replication. One gene thus identified, RAR5, encodes a 175 kDa product, and appears to behave as a repressor of replication origin function. The RAR5 gene is identical to DST2, encoding a strand transferase activity, and KEM1, identified by mutations that enhance the karyogamy defect of a kar\ mutation.
KeywordsReplication Origin Plasmid Stability RAR5 Gene Mitotic Stability Autonomous Replication
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