Resistance and Tolerance of Bacteria to E Colicins

  • J. Smarda
Part of the NATO ASI Series book series (volume 65)

Abstract

It is easy to select colicin non-sensitive mutants from a population of a colicin sensitive strain. In most strains, they occur in relatively high frequencies without induction. It is also easy to exclude colicin immunity as a possible cause of non-sensitivity. However, it is not easy to distinguish between colicin resistance and colicin tolerance. In this study, experimental evidence concerning this problem is presented; we used the indicator strain Escherichia coli K12-Row and its spontaneous mutants selected for decreased sensitivity to colicins E1-E9 as a model system.

Keywords

Agar Defend talC Cyanocobalamin 

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References

  1. Bénédetti H, Frenette M, Baty D, Lloubès R, Geli V, Lazdunski C (1989) Comparison of the uptake systems for the entry of various BtuB group colicins into Escherichia coli J Gen Microbiol 135:3413–3420PubMedGoogle Scholar
  2. Bradbeer C, Woodrow M L, Khalifah LI (1976) Transport of vitamin B12 in Escherichia coli: common receptor system for vitamin B12 and bacteriophage BF23 on the outer membrane of the cell envelope. J. Bacteriol 125:1032–1039PubMedGoogle Scholar
  3. DiMasi D R, White J C, Schnaitman C A, Bradbeer C (1973) Transport of vitamin B12 in Escherichia coli: common receptor sites for vitamin B12 and the E colicins on the outer membrane of the cell envelope. J Bacteriol 115:506–513Google Scholar
  4. Smarda J (1975) Novel approaches to the mode of action of colicins. Folia Microbiol 20:264–271CrossRefGoogle Scholar
  5. Smarda J, Damborsky J (1991) A quantitative assay of group E colicins. Scripta Medica (Brno) 64:111–118Google Scholar
  6. White J C, DiGirolamo P M, Fu M L, Preston Y A, Bradbeer C (1973) Transport of vitamin B12 in Escherichia coli: location and properties of the initial B12-binding site. J Biol Chem 248:3978–3986PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • J. Smarda
    • 1
  1. 1.Deparment of Biology, Faculty of MedicineMasaryk UniversityBrnoCzechoslovakia

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