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Tn5301, A Lactococcal Transposon Encoding Genes for Nisin Biosynthesis

  • H. M. Dodd
  • N. Horn
  • S. Swindell
  • M. J. Gasson
Conference paper
Part of the NATO ASI Series book series (volume 65)

Summary

The genes for nisin biosynthesis are encoded by the conjugative transposon Tn5301. The element is 70kb in size and is located in the chromosome of the nisin-producing strain FI5876. Sequence analysis demonstrated that the left and right termini of Tn5301 were not defined by inverted repeats and that the integrated element was flanked by a directly repeated hexanucleotide target sequence. Integration of the element in the recipient chromosome is site and orientation specific. Two preferred sites have been identified which show some sequence homology. Tn5301 shares features common to other gram-positive elements, eg. Tn916 and Tn554. However, a number of unique properties suggest it may represent the prototype of a novel class of conjugative element.

Keywords

Conjugative Element Tn5301 Insertion Nisin Production Tn5301 Transposition IS904 Copy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Dodd HM, Horn N, Gasson MJ (1990) Analysis of the genetic determinant for production of the peptide antibiotic nisin. J Gen Microbiol 136: 555–566PubMedGoogle Scholar
  2. Dodd HM, Horn N, Swindell S, Gasson MJ (1991) Physical and genetic analysis of the chromosomally located transposon Tn5301, responsible for nisin biosynthesis. In “Lantibiotics” Procedings of the First International Workshop.Google Scholar
  3. Gasson MJ (1984). Transfer of sucrose fermenting ability, nisin resistance and nisin production into Streptococcus lactis 712. FEMS Microbiol Lett 21:7–10CrossRefGoogle Scholar
  4. Fowler GG, Gasson MJ (1991) Antibiotics -nisin. In “Food Preservatives” (eds. N.J. Russell & G.W. Gould) pp 135–152. Blackie, London, UK.Google Scholar
  5. Horn N, Swindell S, Dodd HM, Gasson MJ (1991) Nisin biosynthesis genes are encoded by a novel conjugative transposon. Molec Gen Genet 228:129–135PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • H. M. Dodd
    • 1
  • N. Horn
    • 1
  • S. Swindell
    • 1
  • M. J. Gasson
    • 1
  1. 1.Norwich LaboratoryAFRC Institute of Food ResearchNorwichUK

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