Tn5301, A Lactococcal Transposon Encoding Genes for Nisin Biosynthesis
The genes for nisin biosynthesis are encoded by the conjugative transposon Tn5301. The element is 70kb in size and is located in the chromosome of the nisin-producing strain FI5876. Sequence analysis demonstrated that the left and right termini of Tn5301 were not defined by inverted repeats and that the integrated element was flanked by a directly repeated hexanucleotide target sequence. Integration of the element in the recipient chromosome is site and orientation specific. Two preferred sites have been identified which show some sequence homology. Tn5301 shares features common to other gram-positive elements, eg. Tn916 and Tn554. However, a number of unique properties suggest it may represent the prototype of a novel class of conjugative element.
KeywordsSucrose Recombination Electrophoresis Lactis
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