Replicon Evolution of ColE2-related Plasmids

  • T. Itoh
  • S. Hiraga
Conference paper
Part of the NATO ASI Series book series (volume 65)


The E colicins are a group of bacterial plasmid-coded toxins which use the E. coli cell surface receptor, the btuB gene product. Nine different colicins are distinguished based upon the specificity of interaction of the colicins with their immunity proteins (Males & Stocker, 1982; Cooper & James, 1984). The seventeen plasmids which carry the genes for the E colicins and their immunity proteins are small (about 7 kb, except for plasmids ColE2imm-K317 and ColE6-CT14 which are about 5 kb and 11 kb, respectively), multicopy and nonconjugative (Watsonet al., 1981; Mock & Pugsley, 1982; Cooper & James, 1984). Analyses of these plasmids, using various restriction enzymes, have previously shown that the plasmids ColE2 to E9 are structurally similar (Lawrence & James, 1984; Watson et al., 1985a; Watson et al., 1985b; Chak & James, 1986), whereas plasmid ColEl is distinct (see Chan et al., 1985). More recently, biochemical and nucleotide sequencing analyses of these plasmids have revealed that colicins E2 to E9 are closely related (Toba et al., 1988; Chak et al., 1991; for details see papers by James et al., Lau, and Masaki et al. of this volume). Here we refer to plasmids ColE2 to E9 as the ColE2-related plasmids.


Origin Region Black Arrowhead Homologous Recombination Event Carboxy Terminal Region Basic Replicon 
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  1. Chak K-F, James R (1986) Characterization of the ColE9-J plasmid and analysis of its genetic organization. J Gen Microbiol 132:61–71PubMedGoogle Scholar
  2. Chak K-F, Kuo W-S, Lu F-M, James R (1991) Cloning and characterization of the ColE7 plasmid. J Gen Microbiol 137:91–100PubMedGoogle Scholar
  3. Chan PT, Ohmori H, Tomizawa J, Lebowitz J (1985) Nucleotide sequence and gene organization of ColEl DNA. J Biol Chem 260:8925–8935PubMedGoogle Scholar
  4. Clewell DB, Helinski DR (1972) Effect of growth conditions on the formation of the relaxation complex of supercoiled ColEl deoxyribonucleic acid and protein in Escherichia coli. J Bacteriol 110:1135–1146PubMedGoogle Scholar
  5. Cooper PC, Hawkins FKL, James R (1986) Incompatibility between E colicin plasmids. J Gen Microbiol 132:1859–1862PubMedGoogle Scholar
  6. Cooper PC, James R (1984) Two new E colicins, E8 and E9, produced by a strain of Escherichia coli. J Gen Microbiol 130:209–215PubMedGoogle Scholar
  7. Couturier M, Bex F, Bergquist PL, Maas WK (1988) Identification and classification of bacterial plasmids. Microbiol Rev 52:375–395PubMedGoogle Scholar
  8. Hakkaart MJJ, van den Elzen PJM, Veitkamp E, Nijkamp HJJ (1984) Maintenance of multicopy plasmid CloDF13 mE. coli cells: Evidence for site-specific recombination at par B. Cell 36:203–209PubMedCrossRefGoogle Scholar
  9. Horii T, Itoh T (1988) Replication of ColE2 and ColE3: The regions sufficient for autonomous replication. Mol Gen Genet 212:225–231PubMedCrossRefGoogle Scholar
  10. Inselburg J (1974) Incompatibility exhibited by colicin plasmids El, E2, and E3 in Escherichia coli. J Bacteriol 119:478–483PubMedGoogle Scholar
  11. Itoh T, Horii T (1989) Replication of ColE2 and ColE3 plasmids: In vitro replication dependent on plasmid-coded proteins. Mol Gen Genet 219:249–255PubMedCrossRefGoogle Scholar
  12. Kido M, Yasueda H, Itoh T (1991) Identification of a plasmid-coded protein required for initiation of ColE2 DNA replication. Nucleic Acids Res 19:2875–2880PubMedCrossRefGoogle Scholar
  13. Kingsbury DT, Helinski DR (1970) DNA polymerase as a requirement for maintenance of the bacterial plasmid colicinogenic Factor El. Biochem Biophys Res Commun 41:1538–1544PubMedCrossRefGoogle Scholar
  14. Lawrence GMP, James R (1984) Characterization of the C0IE8 plasmid, a new member of the group E colicin plasmids. Gene 29:145–155PubMedCrossRefGoogle Scholar
  15. Males BM, Stocker BAD (1982) Colicins E4, E5, E6 and A and properties of btuB + colicinogenic transconjugants. J Gen Microbiol 128:95–106PubMedGoogle Scholar
  16. Mock M, Pugsley AP (1982) The btuB group Col plasmids and homology between the colicins they encode. J Bacteriol 150:1069–1076PubMedGoogle Scholar
  17. Persson C, Wagner EGH, Nordstrom K (1988) Control of replication of plasmid Rl: kinetics of in vitro interaction between the antisense RNA, CopA, and its target, CopT. EMBO J 7:3279–3288PubMedGoogle Scholar
  18. Rosen J, Ryder T, Ohtsubo E (1981) Role of RNA transcripts in replication incompatibility and copy number control in antibiotic resistance plasmid derivatives. Nature 290:794–799PubMedCrossRefGoogle Scholar
  19. Saadi S, Maas WK, Hill DF, Bergquist PL (1987) Nucleotide sequence analysis of RepFIC, a basic replicon present in IncFI plasmids P307 and F, and its relation to the RepA replicon of IncFII plasmids. J Bacteriol 169:1836–1846PubMedGoogle Scholar
  20. Stirling CJ, Stewart G, Sherratt DJ (1988) Multicopy plasmid stability in Escherichia coli requires host-encoded functions that lead to plasmid site-specific recombination. Mol Gen Genet 214:80–84PubMedCrossRefGoogle Scholar
  21. Summers DK, Sherratt DJ (1984) Multimerization of high copy number plasmids causes instability: ColEl encodes a determinant essential for plasmid monomerization and stability. Cell 36:1097–1103PubMedCrossRefGoogle Scholar
  22. Summers D, Yaish S, Archer J, Sherratt D (1985) Multimer resolution systems of ColEl and ColK Localization of the crossover site. Mol Gen Genet 201:334–338PubMedCrossRefGoogle Scholar
  23. Tacon W, Sherratt DJ (1976) ColE plasmid replication in DNA polymerase I-deficient strains of Escherichia coli. Mol Gen Genet 147:331–335PubMedCrossRefGoogle Scholar
  24. Tajima Y, Horii T, Itoh, T (1988) Replication of ColE2 and ColE3: Two ColE2 incompatibility functions. Mol Gen Genet 214:451–455PubMedCrossRefGoogle Scholar
  25. Toba M, Masaki H, Ohta T (1988) Colicin E8, a DNase which indicates an evolutionary relationship between colicins E2 and E3. J Bacteriol 170:3237–3242PubMedGoogle Scholar
  26. Tomizawa J (1987) Regulation of ColEl DNA replication by antisense RNA. In: Inouye M, Dudock BS (eds) Molecular Biology of RNA: New perspectives. Academic Press, New York, pp 249–259Google Scholar
  27. Wagner GEH, Nordstrom K (1986) Structural analysis of an RNA molecule involved in replication control of plasmid Rl. Nucleic Acids Res 14:2523–2538PubMedCrossRefGoogle Scholar
  28. Watson R, Rowsome W, Tsao J, Visentin LP (1981) Identification and characterization of Col plasmids from classical colicin E-producing strains. J Bacteriol 147:569–577PubMedGoogle Scholar
  29. Watson RJ, Vernet T, Visentin LP (1985a) Relationships of the Col plasmids E2, E3, E4, E5, E6 and E7:restriction mapping and colicin gene fusions. Plasmid 13:205–210PubMedCrossRefGoogle Scholar
  30. Watson RJ, Vernet T, Visentin LP (1985b) Relationships of the Col plasmids E2, E3, E4, E5, E6 and E7:restriction mapping and colicin gene fusions. Erratum. Plasmid 14:97CrossRefGoogle Scholar
  31. Yasueda H, Horii T, Itoh T (1989) Structural and functional organization of ColE2 and ColE3 replicons. Mol Gen Genet 215:209–216CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • T. Itoh
    • 1
  • S. Hiraga
    • 1
  1. 1.Department of Biology Faculty of ScienceOsaka UniversityToyonaka, Osaka 560Japan

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