Domains of the Escherichia Coli BtuB Protein Involved in Outer Membrane Association and Interaction with Colicin Translocation Components
The lethal action of colicins, which are the bacteriocins active on cells of Escherichia col., can occur through several different mechanisms. Some colicins form a pore or channel in the bacterial cytoplasmic membrane, thereby leading to dissipation of the proton motive force and leakage of cytoplasmic ions and metabolites. Other colicins act in the cytoplasm as nucleases, leading to disruption of the genome or inactivation of ribosomes. Whatever their mechanism, all colicins require specific uptake systems to mediate their attachment to susceptible cells and their efficient translocation to their appropriate sites of action. The multiphasic uptake process is comprised of multiple gene products and provides an experimentally accessible model for a process of profound biological significance, namely, the passage of macromolecules across cellular membranes.
KeywordsPolypeptide Ethanolamine Lyase metE Cobalamin
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