Abstract
Eukaryotic cells manifest many types of motility, which are generally based on either microtubules or microfilaments. The cytostructures formed by these filament systems and also by 10–nm filaments are called cytoskeletons. In many cases, the cytoskeleton is a dynamic structure which is cyclically constructed and destroyed in the cell during its lifetime. Typical examples of both of these cytoskeletons are seen during the course of cell division.
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References
Cao L-g, Wang Y-l (1990) Mechanism of the formation of contractile ring in dividing cultured animal cells. II. Cortical movement of microinjected actin filaments. J Cell Biol 111: 1905–1911
Egelhoff TT, Manstein DJ, Spudich JA (1990) Complementation of myosin null mutants in Dictyostelium discoideum by direct functional selection. Dev Biol 137:359–367.
Endow SA, Henikoff S, Soler-Niedziela L (1990) Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin. Nature 345:81–83
Enos AP, Morris NR (1990) Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans. Cell 60:1019–1027
Hagan IM, Yanagida M (1990) Novel potential mitotic motor protein encoded by the fission yeast cut7 + gene. Nature 347:563–566
Hayden JH, Bowser SS, Rieder CL (1990) Kinetochores capture astral microtubules during chromosome attachment to the mitotic spindle: direct visualization in live newt lung cells. J Cell Biol 111:1039–1045
Hyman AA, Mitchison TJ (1991) Two different microtubule-based motor activities with opposite polarities in kinetochore. Nature 351:206–211
Kubota HY, Itoh K, Asada-Kubota M (1991) Cytological and biochemical analyses of the maternal-effect mutant embryos with abnormal cleavage furrow formation in Xenopus laevis. Dev Biol 144:145–151
McDonald HB, Stewart RJ, Goldstein LSB (1990) The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor. Cell 63:1159–1165
Oakley BR, Oakley CE, Yoon Y, Jung MK (1990) γ-Tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans. Cell 61:12891301
Pfarr CM, Coue M, Grissom PM, Hays TS, Porter ME, Mcintosh JR (1990) Cytoplasmic dynein is localized to kinetochores during mitosis. Nature 345:263–265
Pluta AF, Cook CA, Earnshaw WC (1990) Structure of the human centromere at metaphase. Trends Biol Sci 15:181–185
Sawin KE, Mitchison TJ (1991a) Mitotic spindle assembly by two different pathways in vitro. J Cell Biol 112:925–940
Sawin KE, Mitchison TJ (1991b) Poleward microtubule flux in mitotic spindles assembled in vitro. J Cell Biol 112:941–954
Steuer ER, Wordeman L, Schroer TA, Sheets MP (1990) Localization of cytoplasmic dynein to mitotic spindles and kinetochores. Nature 345:266–268
Vale RD (1991) Severing of stable microtubules by mitotically activated protein in Xenopus egg extracts. Cell 64:827–839
Walker RA, Salmon ED, Endow SA (1990) The Drosophila claret segregation protein is a minus-end directed motor molecule. Nature 347:780–782
Zhang P, Knowles BA, Goldstein LSB, Hawleys RS (1990) A kinesin-like protein required for distributive chromosome segregation in Drosophila. Cell 62:1053–1062
Zheng Y, Jung MK, Oakley BR (1991) γ-Tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65:817–823
Allen C, Borisy GG (1974) Structural polarity and directional growth of microtubules of Chlamydomonas flagella. J Mol Biol 90:381–402
Allen RD, Bajer A, LaFountain J (1969) Poleward migration of particles or states in spindle fiber filaments during mitosis in Haemanthus. J Cell Biol 43:4a
Amos LA, Klug A (1974) The arrangement of subunits in flagellar microtubules. J Cell Sci 14:523–549
Arnold JM (1969) Cleavage furrow formation in a telolecthal egg (Loligo pealli). J Cell Biol 41:894–904
Aubin JE, Osborn M, Weber K (1979) Inhibition of cytokinesis and altered contractile ring morphology induced by cytochalasins in synchronized PtK2 cells. Exp Cell Res 136:63–79
Bajer A (1973) Interaction of microtubules and the mechanism of chromosome movement (zipper hypothesis). Cytobios 8:139–160
Bajer A (1982) Functional autonomy of monopolar spindle and evidence of oscillatory movement in mitosis. J Cell Biol 93:33–48
Bajer A, Mole-Bajer J (1969) Formation of spindle fibers, kinetochore orientation, and behavior of the nuclear envelope during mitosis in endosperm. Chromosoma 27:448–484
Bajer A, Molé-Bajer J (1972) Spindle dynamics and chromosome movements. Int Rev Cytol Suppl 3:1–271
Balczon RD, Brinkley BR (1987) Tubulin interaction with kinetochore protein: analysis by in vitro assembly and chemical cross-linking. J Cell Biol 105:855–862
Bergen LG, Borisy GG (1980) Head-to-tail polymerization of microtubules in vitro: electron microsope analysis of seeded assembly. J Cell Biol 84:141–150
Bergen LG, Kuriyama R, Borisy GG (1980) Polarity of microtubules nucleated by centrosomes and chromosomes of CHO cells in vitro. J Cell Biol 84:151–159
Bonder EM, Fishkind DJ, Cotran NM, Begg DA (1989) The cortical actin-membrane cytoskeleton of unfertilized sea urchin eggs: analysis of the spatial organization and relationship of filamentous actin, nonfilamentous actin, and egg spectrin. Dev Biol 134:327–341
Brinkley BR, Kartwright J (1975) Cold-labile and cold-stable microtubules in the mitotic spindle of mammalian cells. Ann N Y Acad Sci 253:109–123
Brinkley BR, Zinkowski RP, Mollon WL, Davis FM, Pisegna MA, Pershouse M, Rao PN (1988) Movement and segregation of kinetochores experimentally detached from mammalian chromosomes. Nature 336:251–254
Brinkley BR, Valdivia MM, Tousson A, Balczon RD (1989) The kinetoehore: structure and molecular organization. In: Hyams JS, Brinkley BR (eds) Mitosis, molecules and mechanisms. Academic Press, New York, pp 77–118
Cande WZ (1980) A permeabilized cell model for studying cytokinesis using mammalian tissue culture cells. J Cell Biol 87:326–335
Cande WZ (1982) Nucleotide requirements for anaphase chromosome movements in permeablized mitotic cells: anaphase B but not anaphase A requires ATP. Cell 28:15–22
Cande WZ, McDonald KL (1985) In vitro reactivation of anaphase spindle elongation using isolated diatom spindles. Nature 316:168–170
Cande WZ, Baskin T, Hogan C, McDonald KL, Masuda H, Wordeman L (1989) In vitro analysis of anaphase spindle elongation. In: Warner FD, Mcintosh JR (eds) Cell movement, vol 2. Kinesin, dynein and microtubule dynamics. Alan R Liss, New York, pp 441–452
Carlier M-F, Hill TL, Chen Y-D (1984) Interference of GTP hydrolysis in the mechanism of microtubule assembly: an experimental study. Proc Natl Acad Sci USA 81:771–775
Cassimeris L, Pryer NK, Salmon ED (1988) Real-time observations of microtubule dynamic instability in living cells. J Cell Biol 107:2223–2231
Dan K (1943) Behavior of the cell surface during cleavage. VI. On the mechanism of cell division. J Fac Sci Tokyo Imp Univ Sec 4, 6:323–368
Dan K (1988) Mechanism of equal cleavage of sea urchin egg: transposition from astral mechanism to constricting mechanism. Zool Sci 5:507–517
De Lozanne A, Spudich JA (1987) Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. Science 236:1086–1091
Devore JJ, Conrad GW, Rappaport R (1990) A model for astral stimulation of cytokinesis in animal cells. J Cell Biol 109:2225–2232
Dinsmore JH, Sloboda RD (1988) Calcium and calmodulin-dependent phosphorylation of a 62 kD protein induces microtubule depolymerization in sea urchin mitotic apparatus. Cell 53:769–780
Euteneuer U, Mcintosh JR (1981) Structural polarity of kinetoehore microtubules in PtK1 cells. J Cell Biol 89:338–345
Euteneuer U, Ris H, Borisy GG (1983) Polarity of kinetoehore microtubules in Chinese hamster ovary cells after recovery from a colcemid block. J Cell Biol 97:202–208
Forer A (1965) Local reduction of spindle fiber birefringence in living Nephrotoma suturalis spermatocytes induced by ultraviolet microbeam irradiation. J Cell Biol 25:95–117
Fujiwara K, Pollard TD (1976) Fluorescent antibody localization of myosin in the cytoplasm, cleavage furrow, and mitotic spindle of human cells. J Cell Biol 71:848–875
Fujiwara K, Porter ME, Pollard TD (1978) Alpha-actinin localization in the cleavage furrow during cytokinesis. J Cell Biol 79:268–275
Fukui Y, Lynch TJ, Brzeska H, Korn ED (1989) Myosin I is located at the leading edges of locomoting Dictyostelium amoebae. Nature 341:328–331
Gorbsky GJ, Borisy GG (1989) Microtubules of the kinetoehore fiber turn over in metaphase but not in anaphase. J Cell Biol 109:653–662
Gorbsky GJ, Sammak PJ, Borisy GG (1987) Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetoehore ends. J Cell Biol 104:9–18
Gorbsky GJ, Sammak PJ, Borisy GG (1988) Microtubule dynamics and chromosome motion visualized in living anaphase cells. J Cell Biol 106:1185–1192
Haimo LT, Telzer BR (1981) Dynein-microtubule interactions. ATP-sensitive dynein binding and the structural polarity of mitotic microtubules. Cold Spring Harbor Symp Quant Biol 46:207–218
Hamaguchi MS, Hamaguchi Y, Hiramoto T (1986) Microinjected polystylene beads move along astral rays in sand dollar eggs. Dev Growth Differ 28:461–470
Hamaguchi Y (1975) Microinjection of colchicine into sea urchin eggs. Dev Growth Differ 17:111–117
Hamaguchi Y, Mabuchi I (1982) Effects of phalloidin microinjection and localization of fluorescein-labeled phalloidin in living sand dollar eggs. Cell Motil 2:103–113
Hamaguchi Y, Toriyama M, Sakai H, Hiramoto Y (1987) Redistribution of fluoreseently labeled tubulin in the mitotic apparatus of sand dollar eggs and the effects of taxol. Cell Struct Funct 12:43–52
Hays TS, Salmon ED (1990) Poleward force at the kinetochore in metaphase depends on the number of kinetochore microtubules. J Cell Biol 110:391–404
Hays TS, Wise D, Salmon ED (1982) Traction force on a kinetochore at metaphase acts as a linear function of kinetochore fiber length. J Cell Biol 93:374–382
Henson JH, Begg DA, Beaulieu SM, Fishkind DJ, Bonder EM, Terasaki M, Lebeche DL, Kaminer B (1989) A calsequestrin-like protein in the endoplasmic reticulum of the sea urchin: localization and dynamics in the egg and first cell cycle embryo. J Cell Biol 109:149–161
Hiramoto Y (1956) Cell division without mitotic apparatus in sea urchin eggs. Exp Cell Res 11:630–636
Hiramoto Y (1975) Force exerted by the cleavage furrow of sea urchin eggs. Dev Growth Differ 17:27–38
Hiramoto Y (1979) Mechanical properties of the dividing sea urchin egg. In: Hatano S, Ishikawa H, Sato H (eds) Cell motility: molecules and organization. University of Tokyo Press, Tokyo, pp 653–663
Hiramoto Y, Izutsu K (1977) Poleward movement of “markers” existing in mitotic spindles of grasshopper spermatocytes. Cell Struct Funct 2:257–259
Hiramoto Y, Shoji Y (1982) Location of the motive force for chromosome movement in sand dollar eggs. In: Sakai H, Mohri H, Borisy GG (eds) Biological functions of microtubules and related structures. Academic Press, New York, pp 247–259
Hisanaga S, Sakai H (1980) Cytoplasmic dynein of the sea urchin egg. I. Dev Growth Differ 22:373–384
Horio T, Hotani H (1986) Visualization of the dynamic instability of individual microtubules by dark-field microscopy. Nature 321:605–607
Hotani H, Horio T (1988) Dynamics of microtubules visualized by dark field microscopy: treadmilling and dynamic instability. Cell Motil Cytoskelet 10:229–236
Huitorel P, Kirschner MW (1988) The polarity and stability of microtubule capture by the kinetochore. J Cell Biol 106:151–159
Inoué S (1981) Cell division an the mitotic spindle. J Cell Biol 91:131s-147s
Inoué S, Ritter H (1975) Dynamics of mitotic spindle organization and function. In: Inoué S, Stephens RE (eds) Molecules and cell movement. Raven, New York, pp 3–29
Inoué S, Sato H (1967) Cell motility by labile association of molecules. The nature of mitotic spindle fibers and their role in chromosome movement. J Gen Physiol 50:259–292
Ishidate S, Mabuchi I (1988a) A novel actin filament-capping protein from sea urchin eggs: a 20000-moleeular weight protein-actin complex. J Biochem 104:72–80
Ishidate S, Mabuchi I (1988b) Localization and possible function of 20kDa actin-modulating protein (actolinkin) in the sea urchin egg. Eur J Biochem 46:275–281
Ishimoda-Takagi (1979) Localization of tropomyosin in sea urchin eggs. Exp Cell Res 119:423–428
Ishimoda-Takagi (1984) Evidence for the involvement of muscle tropomyosin in the contractile elements of the coelomesophagus complex in sea urchin embryos. Dev Biol 105: 365–376
Jameson L, Frey T, Zeeberg B, Dalldorf F, Caplow M (1980) Inhibition of microtubule assembly by phosphorylation of microtubule-associated proteins. Biochemistry 19:2472–2479
Katoh K, Ishikawa H (1989) The cytoskeletal involvement in cellularization of the Drosophila melanogaster embryo. Protoplasma 150:83–95
Kiehart DP, Mabuchi I, Inoué S (1982) Evidence that myosin does not contribute to force production in chromosome movement. J Cell Biol 94:165–178
Knabe T, Kobayashi I, Tanaka K (1989) Dynamics of cytoplasmic organelles in the cell cycle of the fission yeast Schizosaccharomyces pombe: three dimensional reconstruction from serial sections. J Cell Sci 94:647–656
Knecht DA, Loomis WF (1987) Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum. Science 236:1081–1086
Koshland DE, Mitchison TJ, Kirschner MW (1988) Polewards chromosome movement driven by microtubule depolymerization in vitro. Nature 331:499–504
Kron SJ, Spudich JA (1986) Fluorescent actin filaments move on myosin fixed to a glass surface. Proc Natl Acad Sci USA 83:6272–6276
Kuriyama R (1984) Activity and stability of centrosomes in Chinese hamster ovary cells in nucleation of microtubules in vitro. J Cell Sci 66:277–295
Kuriyama R, Borisy GG (1985) Identification of molecular components of the centrosphere in the mitotic spindle of sea urchin eggs. J Cell Biol 101:524–530
Langanger G, De Mey J, Moeremans M, Daneeis G, De Brabander M, Small JV (1984) Ultrastructural localization of α-actinin and filamin in cultured cells with the immunogold staining (IGS) method. J Cell Biol 99:1324–1334
Mabuchi I (1973) ATPase in the cortical layer of sea urchin egg: its properties and interaction with cortex protein. Biochim. Biophys Acta 297:317–332
Mabuchi I (1986) Biochemical aspects of cytokinesis. Int Rev Cytol 101:175–213
Mabuchi I (1990) Cleavage furrow formation and actin-modulating proteins. Ann N Y Acad Sci 582:131–146
Mabuchi I, Kane RE (1987) A 250K-molecular weight actin-binding protein from actin-based gels formed in sea urchin egg cytoplasmic extract. J Biochem 102:947–956
Mabuchi I, Okuno M (1977) The effect of myosin antibody on the cell division of starfish blastomeres. J Cell Biol 74:251–263
Mabuchi I, Takano-Ohmuro H (1990) Effects of inhibitors of myosin light chain kinase and other protein kinases on the first cell division of sea urchin eggs. Dev Growth Differ 32:549–556
Mabuchi I, Hosoya H, Sakai H (1980) Actin in the cortical layer of the sea urchin egg. Changes in its content during and after fertilization. Biomed Res 1:417–426
Mabuchi I, Hamaguchi Y, Kobayashi T, Hosoya H, Tsukita S, Tsukita S (1985) Alpha-actinin from sea urchin eggs: biochemical properties, interaction with actin, and distribution in the cell during fertilization and cleavage. J Cell Biol 100:375–383
Mabuchi I, Tsukita S, Tsukita S, Sawai T (1988) Cleavage furrow isolated from newt eggs: contraction, organization of actin filaments, and protein components of the furrow. Proc Nat Acad Sci USA 85:5966–5970
Maekawa S, Endo S, Sakai H (1987) A high molecular weight actin binding protein: its localization in the cortex of the sea urchin egg. Exp Cell Res 172:340–353
Manstein DJ, Titus MA, De Lozanne A, Spudich JA (1989) Gene replacement in Dictyostelium: generation of myosin null mutants. EMBO J 8:923–932
Margolis RL, Wilson L (1978) Opposite end assembly and disassembly of microtubules at steady-state in vitro. Cell 13:1–8
Margolis RL, Wilson L (1981) Microtubule treadmills - possible molecular machinery. Nature 298:705–711
Margolis RL, Wilson L, Kiefer BI (1978) Mitotic mechanism based on intrinsic microtubule behavior. Nature 272:450–452
Marsland D, Landau JV (1954) The mechanism of cytokinesis: temperature-pressure studies on the cortical gel system in various marine eggs. J Exp Zool 125:507–539
Masuda H, Cande WZ (1987) The role of tubulin polymerization during spindle elongation in vitro. Cell 49:193–202
Masuda H, McDonald KL, Cande WZ (1988) The mechanism of anaphase spindle elongation: uncoupling of tubulin incorporation and microtubule sliding during in vitro spindle reactivation. J Cell Biol 107:623–633
Masuda H, Hirano T, Yanagida M, Cande WZ (1990) In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells. J Cell Biol 110:417–425
Maupin P, Pollard TD (1986) Arrangement of actin filaments and myosin-like filaments in the contractile ring and of actin-like filaments in the mitotic spindle of dividing HeLa cells. J Ultrastruct Mol Struct Res 94:92–103
Mazia D (1987) The chromosome cycle and the centrosome cycle in the mitotic cycle. Int Rev Cytol 100:49–92
Mazia D, Dan K (1952) The isolation and biochemical characterization of the mitotic apparatus of dividing cells. Proc Natl Acad Sci USA 38:826–838
McCaffrey G, Vale RD (1989) Identification of a kinesin-like microtubule-based motor protein in Dictyostelium discoideum. EMBO J 8:3229–3234
McDonald KL, Pickett-Heaps JD, Mcintosh JR, Tippit DH (1977) On the mechanism of anaphase spindle elongation in Diatom vulgare. J Cell Biol 74:377–388
Mcintosh JR (1985) Spindle structure and the mechanism of chromosome movement. In “Aneuploidy: Etiology and Mechanism” Dellarco V, Voytek PE and Hollander A, eds. Plenum, New York, pp 197–229
Mcintosh JR, Vigers GPA, Hays TS (1989) Dynamic behavior of mitotic microtubules. In: Warner FD, Mcintosh JR (eds) Cell movement, vol 2. Kinesin, dynein and microtubule dynamics. Alan R Liss, New York, pp 371–382
Mitchison TJ (1989a) Chromosome alignment at mitotic metaphase: balanced forces or smart kinetochores? In: Warner FD, Mcintosh JR (eds) Cell movement, vol 2. Kinesin, dynein and microtubule dynamics. Alan R Liss, New York, pp 421–430
Mitchison TJ (1989b) Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence. J Cell Biol 109:637–652
Mitchison TJ, Kirschner MW (1984a) Microtubule assembly nucleated by isolated centrosomes. Nature 312:232–237
Mitchison TJ, Kirschner MW (1984b) Dynamic instability of microtubule growth. Nature 312:237–242
Mitchison TJ, Kirschner MW (1985a) Properties of the kinetochore in vitro. I. Microtubule nucleation and tubulin binding. J Cell Biol 101:755–765
Mitchison TJ, Kirschner MW (1985b) Properties of the kinetochore in vitro. II. Microtubule capture and ATP dependent translocation. J Cell Biol 101:766–777
Mitchison TJ, Evans L, Schulze E, Kirschner MW (1986) Sites of microtubule assembly and disassembly in the mitotic spindle. Cell 45:515–527
Mittal B, Sanger JM, Sanger JW (1987) Visualization of myosin in living cells. J Cell Biol 105:1753–1760
Molé-Bajer J (1975) The role of centrioles in the development of the astral spindle (newt). Cytobios 13:117–140
Nicklas RB (1971) Mitosis. In: Prescott DM, Goldstein L, McConkey E (eds) Advances in cell biology, vol 2. Appleton-Century-Crofts, New York, pp 225–297
Nunnally MH, D’Angelo, JM, Craig SW (1980) Filamin concentration in cleavage furrow and midbody region: frequency of occurrence compared with that of alpha-actinin and myosin. J Cell Biol 87:219–226
Ohta K, Toriyama M, Endo S, Sakai H (1988) Localization of mitotic-apparatus-associated 51-kD protein in unfertilized and fertilized sea urchin eggs. Cell Motil Cytoskelet 10: 496–505
Ohta K, Toriyama M, Miyazaki M, Murofushi H, Hosoda S, Endo S, Sakai H (1990) The mitotic apparatus-associated 51-kDa protein from sea urchin eggs is a GTP-binding protein and is immunologically related to yeast polypeptide elongation factor la. J Biol Chem 265:3240–3247
Östergren G (1951) The mechanism of co-orientation in bivalents and multivalents. The theory of orientation by pulling. Hereditas 37:85–156
Paschal BM, Vallee RB (1987) Retrograde transport by the microtubule-associated protein MAP 1C. Nature 330:181–183
Pickett-Heaps JD, Tippit DH (1978) The diatom spindle in perspective. Cell 14:455–467
Pickett-Heaps JD, Tippit DH, Porter KR (1982) Rethinking mitosis. Cell 29:729–744
Pratt MM, Otter T, Salmon ED (1980) Dynein-like Mg2+-ATPase in mitotic spindles isolated from sea urchin embryos (Strongylocentrotus droebachiensis). J Cell Biol 86:738–745
Rappaport R (1961) Experiments concerning the cleavage stimulus in sand dollar eggs. J Exp Zool 148:81–89
Rappaport R (1967) Cell division: direct measurement of maximum tension exerted by furrow of echinoderm eggs. Science 156:1241–1243
Rappaport R (1969) Division of isolated furrows and furrow fragments in invertebrate eggs. Exp Cell Res 56:87–91
Rappaport R (1971) Cytokinesis in animal cells. Int Rev Cytol 31:169–213
Rappaport R (1973) On the rate of the cleavage stimulus in sand dollar eggs. J Exp Zool 183:115–120
Rebhun LI, Palazzo RE (1988) In vitro reactivation of anaphase B in isolated spindles of the sea urchin egg. Cell Motil Cytoskelet 10:197–209
Rieder CL (1981) The structure of cold-stable kinetoehore fiber in metaphase PtK1 cells. Chromosoma 84:145–158
Rieder CL (1982) The formation, structure and composition of the mammalian kinetoehore and kinetoehore fiber. Int Rev Cytol 79:1–57
Rieder CL, Alexander SP (1990) Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells. J Cell Biol 110:81–95
Roos U-P (1976) Light and electron microscopy of rat kangaroo cells in mitosis. III. Patterns of chromosome behavior during prometaphase. Chromosoma 54:363–385
Sakai H (1978a) Isolated mitotic apparatus and chromosome motion. Int Rev Cytol 55:23–48
Sakai H (1978b) Induction of chromosome motion in the isolated mitotic apparatus as a function of microtubules. In: Dirkson ER, Prescott DM, Fox CF (eds) Cell reproduction: in honor of Daniel Mazia. Academic Press, New York, pp 425–432
Sakai H, Hiramoto Y, Kuriyama R (1975) The glycerol-isolated mitotic apparatus: a response to porcine brain tubulin and induction of chromosome motion. Dev Growth Differ 17:265–274
Sakai H, Mabuchi I, Shimoda S, Kuriyama R, Ogawa K, Mohri H (1976) Induction of chromosome motion in the glycerol-isolated mitotic apparatus: nucleotide specificity and effects of antidynein and myosin sera on the motion. Dev Growth Differ 18:211–219
Salmon ED, McKeel M, Hays T (1984a) The rapid rate of tubulin dissociation from microtubules in the mitotic spindle in vivo measured by blocking polymerization with colchicine. J Cell Biol 99:1066–1075
Salmon ED, Leslie RJ, Saxton WM, Karow ML, Mcintosh JR (1984b) Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching. J Cell Biol 99:2165–2174
Sammak PJ, Borisy GG (1988) Direct observation of microtubule dynamics in living cells. Nature 332:724–726
Sanger JM, Sanger JW (1980) Banding and polarity of actin filaments in interphase and cleaving cells. J Cell BioL86:568–575
Saxton WM, Mcintosh JR (1987) Interzone microtubule behavior in late anaphase and telophase spindles. J Cell Biol 105:875–886
Schatten H, Walter M, Mazia D, Biessmann H, Paweletz N, Coffe G, Schatten G (1987) Centrosome detection in sea urchin eggs with a monoclonal antibody against Drosophila intermediate filament proteins: characterization of stages of the division cycle of centrosomes. Proc Natl Acad Sci USA 84:8488–8492
Scholey JM, Neighbors B, Mcintosh JR, Salmon ED (1985a) Isolation of microtubules and a dynein-like MgATPase from unfertilized sea urchin eggs. J Biol Chem 259:6516–6525
Scholey JM, Porter ME, Grissom PM, Mcintosh JR (1985b) Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle. Nature 318:483–486
Schroeder TE (1968) Cytokinesis: filaments in the cleavage furrow. Exp Cell Res 53:272–276
Schroeder TE (1970) The contractile ring. I. Fine structure of dividing mammalian (HeLa) cells and the effects of cytochalasin B. Z Zellforsch Mikrosk Anat 109:431–449
Schroeder TE (1972) The contractile ring. II. Determining its brief existence, volumetric changes, and vital role in cleaving Arbacia eggs. J Cell Biol 53:419–434
Schroeder TE (1975) Dynamics of the contractile ring. In: Inoué S, Stephens RE (eds) Molecules and cell movement. Raven, New York, pp 305–334
Schroeder TE (1986) The egg cortex in early development of sea urchins and starfish. In: Browder LW (ed) Developmental biology, vol 2. Plenum, New York, pp 59–100
Schroeder TE (1987) Fourth cleavage of sea urchin blastomeres: microtubule patterns and myosin localization in equal and unequal cell divisions. Dev Biol 124:9–22
Schroeder TE, Otto J J (1988) Immunofluorescent analysis of actin and myosin in isolated contractile rings of sea urchin eggs. Zool Sci 5:713–725
Schulze E, Kirschner MW (1986) Microtubule dynamics in interphase cells. J Cell Biol 102:1020–1031
Schulze E, Kirschner MW (1988) New features of microtubule behavior observed in vivo. Nature 334:356–359
Shpetner HS, Vallee RB (1989) Identification of dynamin, a novel mechanochemical enzyme that mediates interactions between microtubules. Cell 59:421–432
Sluder G, Rieder CL (1985) Experimental separation of pronuclei in fertilized sea urchin eggs: chromosomes do not organize a spindle in the absence of centrosomes. J Cell Biol 100:897–903
Soltys BJ, Borisy GG (1985) Polymerization of tubulin in vivo: direct evidence for assembly onto microtubule ends and from centrosomes. J Cell Biol 100:1682–1689
Steffen W, Fuge H, Dietz R, Bastmeyer M, Muller G (1986) Aster-free spindle poles in insect spermatocytes: evidence for chromosome-induced spindle formation? J Cell Biol 102:1679–1687
Swann MM, Mitchison JM (1953) Cleavage of sea-urchin eggs in colchicine. J Exp Biol 35:506–513
Tilney LG, Marsland D (1969) A fine structural analysis of cleavage induction and furrowing in the eggs of Arbacia punctulata. J Cell Biol 44:170–184
Toriyama M, Ohta K, Endo S, Sakai H (1987) 51k Protein, a component of microtubule-organizing granules (MTOGs) in the mitotic apparatus involving in aster formation in vitro. Cell Motil Cytoskelet 9:117–128
Usui N, Yoneda M (1982) Ultrastructural basis of the tension increase in sea urchin eggs prior to cytokinesis. Dev Growth Differ 24:453–465
Vale RD, Reese TS, Sheetz MP (1985) Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Cell 42:39–50
Vandre DD, Borisy GG (1989) The centrosome cycle in animal cells. In: Hyams JS, Brinkley BR (eds) Mitosis. Molecules and mechanisms. Academic Press, New York, pp 39–75
Vigers, GPA, Coue M, Mcintosh JR (1988) Fluorescent microtubules break up under illumination. J Cell Biol 107:1011–1024
Wadsworth P, Salmon ED (1986) Analysis of the treadmilling model during metaphase of mitosis using fluorescence redistribution after photobleaching. J Cell Biol 102:1032–1038
Walker RA, O’Brien ET, Pryer NK, Soboeiro MF, Voter WA, Erickson HP, Salmon ED (1988) Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies. J Cell Biol 107:1437–1448
Wallraffe E, Schleicher M, Modersitzki M, Rieger D, Isenberg G, Gerisch G (1986) Selection of Dyctiostelium mutants defective in cytoskeletal proteins: use of an antibody that binds to the ends of α-actinin rods. EMBO J 5:61–67
Weisenberg R, Taylor EW (1968) Studies on ATPase activity of sea urchin eggs and the isolated mitotic apparatus. Exp Cell Res 53:372–384
Witt PL, Ris H, Borisy GG (1981) Structure of kinetochore fibers: microtubule continuity and intermicrotubule bridges. Chromosoma 83:523–540
Wordeman L, Cande WZ (1987) Reactivation of spindle elongation in vitro is correlated with the phosphorylation of a 205 kD spindle-associated protein. Cell 50:535–543
Yamamoto H, Fukunaga K, Tanaka E, Miyamoto E (1983) Ca2+- and calmodulin-dependent phosphorylation of microtubule-associated protein 2 and tau factor, and inhibition of microtubule assembly. J Neurochem 41:1119–1125
Yanagida T, Arata T, Oosawa F (1985) Sliding distance of actin filament induced by a myosin crossbridge during one ATP hydrolysis cycle. Nature 316:366–369
Yoneda M, Dan K (1972) Tension at the surface of the dividing sea-urchin egg. J Exp Biol 57:575–587
Yonemura S (1989) Morphological and biochemical analyses of the cortical cytoskeleton and of the contractile ring in the sea urchin egg. Thesis, University of Tokyo
Yonemura S, Kinoshita S (1986) Actin filament organization in the sand dollar egg cortex. Dev Biol 115:171–183
Yonemura S, Mabuchi I, Tsukita S (1991) Mass isolation of cleavage furrows from dividing sea urchin eggs. J Cell Sci 100:73–84
Yumura S, Fukui Y (1985) Reversible cyclic AMP-dependent change in distribution of myosin thick filaments in Dictyostelium. Nature 314:194–196
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© 1992 Springer-Verlag Berlin Heidelberg
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Mabuchi, I., Itoh, T.J. (1992). Molecular Mechanisms of Mitosis and Cytokinesis. In: Sugi, H. (eds) Muscle Contraction and Cell Motility. Advances in Comparative and Environmental Physiology, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76927-6_8
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DOI: https://doi.org/10.1007/978-3-642-76927-6_8
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