Molecular Cloning and Expression of CD 34: A Haemopoietic Progenitor-Associated Cell Surface Glycoprotein

  • M. F. Greaves
  • J. Brown
  • L. Fina
  • D. Robertson
  • D. Delia
  • H. V. Molgaard
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 35)


The CD 34 group of monoclonal antibodies recognize a 105–120 kDa cell surface glycoprotein which is selectively expressed by human myeloid and lymphoid progenitor cells probably including the haemopoietic stem cell [1–3] (for a recent review see [4]). CD 34 antibodies therefore offer a prospect of using highly enriched human stem cells for analysis of their regulation [5–7], gene transfection and/or transplantation [8]. The CD 34 antigen is also expressed on vascular endothelial cells [9].


Haemopoietic Stem Cell Stem Cell Antigen Lymphoid Progenitor Cell Haemopoietic Progenitor Cell Leukaemia Research Fund 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH (1984) Antigenic analysis of hematopoiesis. III. A Hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol 133:157PubMedGoogle Scholar
  2. 2.
    Katz FE, Tindie R, Sutherland DR, Greaves MF (1985) Identification of a membrane glycoprotein associated with haemopoietic progenitor cells. Leuk Res 9:191PubMedCrossRefGoogle Scholar
  3. 3.
    Andrews RG, Singer JW, Bernstein ID (1986) Monoclonal antibody 12–8 recognizes a 115-kd molecule present on both unipotent and multipotent hematopoietic colony-forming cells and their precursors. Blood 67:842PubMedGoogle Scholar
  4. 4.
    Civin CI, Trischmann TM, Fackler MJ, Bernstein ID, Bühring H-J, Campos L, Greaves MF, Kamoun M, Katz DR, Lansdorp PM, Look AT, Seed B, Sutherland DR, Tindie RW, Uchanska-Ziegler B (1989) Report on the CD 34 cluster workshop. In: Knapp W, Dörken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (eds) Leucocyte typing IV. Oxford University Press, Oxford, p 818Google Scholar
  5. 5.
    Andrews RG, Singer JW, Bernstein ID (1990) Human hematopoietic precursors in long-term culture: single CD 34+ cells that lack detectable T cell, B cell, and myeloid cell antigens produce multiple colony-forming cells when cultured with marrow stromal cells. J Exp Med 172:355PubMedCrossRefGoogle Scholar
  6. 6.
    Caux C, Saeland S, Favre C, Duvert V, Mannoni P, Banchereau J (1990) Tumor necrosis factor-alpha strongly potentiates interleukin-3 and granulocyte-macrophage colony-stimulating factor-induced proliferation of human CD34+ hematopoietic progenitor cells. Blood 75:2292PubMedGoogle Scholar
  7. 7.
    Civin CI, Strauss LC, Fackler MJ, Trischmann TM, Wiley JM, Loken MR (1990) Positive stem cell selection-basic science. Prog Clin Biol Res 333:387PubMedGoogle Scholar
  8. 8.
    Berenson RJ, Andrews RG, Bensinger WI, Kalamasz D, Knitter G, Buckner CD, Bernstein ID (1988) Antigen CD34+ marrow cells engraft lethally irradiated baboons. J Clin Invest 81:951PubMedCrossRefGoogle Scholar
  9. 9.
    Fina L, Molgaard HV, Robertson D, Bradley NJ, Monaghan P, Delia D, Sutherland DR, Baker MA, Greaves MF (1990) Expression of the CD 34 gene in vascular endothelial cells. Blood 75:2417PubMedGoogle Scholar
  10. 10.
    Sutherland DR, Watt SM, Dowden G, Karhi K, Baker MA, Greaves MF, Smart JE (1988) Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD 34. Leukemia 2:793PubMedGoogle Scholar
  11. 11.
    Watt SM, Karhi K, Gatter K, Furley AJW, Katz FE, Healy LE, Altass LJ, Bradley NJ, Sutherland DR, Levinsky R, Greaves MF (1987) Distribution and epitope analysis of the cell membrane glycoprotein (HPCA-1) associated with human hemopoietic progenitor cells. Leukemia 1:417PubMedGoogle Scholar
  12. 12.
    Molgaard HV, Spurr NK, Greaves MF (1989) The hemopoietic stem cell antigen, CD 34, is encoded by a gene located on chromosome 1. Leukemia 3:773PubMedGoogle Scholar
  13. 13.
    Tenen DG, Satterthwaite AB, Borson R, Simmons D, Eddy RL, Shows TB (1990) Chromosome 1 localization of the gene for CD 34, a surface antigen of human stem cells. Cytogenet Cell Genet 53:55PubMedCrossRefGoogle Scholar
  14. 14.
    Brown J, Greaves MF, Molgaard HV (1991) The gene encoding the stem cell antigen, CD 34, is conserved in mouse and expressed in haemopoietic progenitor cell lines, brain and embryonic fibroblasts. Int Immunol 3:175PubMedCrossRefGoogle Scholar
  15. 15.
    Spooncer E, Heyworth CM, Dunn A, Dexter TM (1986) Self-renewal and differentiation of interleukin-3—dependent multipotent stem cells are modulated by stromal cells and serum factors. Differentiation 31:111PubMedCrossRefGoogle Scholar
  16. 16.
    Schlingemann RO, Rietveld FJR, de Waal RMW, Bradley NJ, Skene AI, Davies AJS, Greaves MF, Denekamp J, Ruiter DJ (1990) Leukocyte antigen CD 34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal micro-processes in the tumor stroma. Lab Invest 62:690PubMedGoogle Scholar
  17. 17.
    Furley AJ, Reeves BR, Mizutani S, Altass LJ, Watt SM, Jacob MC, van den Elsen P, Terhorst C, Greaves MF (1986) Divergent molecular phenotypes of KG 1 and KG 1a myeloid cell lines. Blood 68:1101PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • M. F. Greaves
  • J. Brown
  • L. Fina
  • D. Robertson
  • D. Delia
  • H. V. Molgaard
    • 1
  1. 1.Leukaemia Research Fund CentreInstitute of Cancer Research, Chester Beatty LaboratoriesLondonUK

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