Abstract
The standard situation is where the researcher has a DNA sequence which may or may not code for a known gene product. The sequence is read into a frame, called an open reading frame or ‘orf’ consisting of a long run of amino acid codons with no terminator codon. The problem is usually to find if this unidentified reading frame or ‘urf’ really codes for a protein, and to find if this protein is known to exist in nature. Two approaches to this problem exist. Firstly one can do computer searching. Or secondly one can use antibodies raised against synthetic peptides “patterned on the sequence of the expected gene product” (Doolittle, 1986). This section will introduce the first method.
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© 1991 Springer-Verlag Berlin Heidelberg
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Sillince, J.A.A., Sillince, M. (1991). Sequence Searching. In: Molecular Databases for Protein Sequences and Structure Studies. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76809-5_9
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DOI: https://doi.org/10.1007/978-3-642-76809-5_9
Publisher Name: Springer, Berlin, Heidelberg
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