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Molecular Biology

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Drosophila Genetics

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Abstract

The foregoing chapters of this book describe classical genetic experiments and analyses making use of Drosophila as an easy and inexpensive experimental animal. Its short generation time, ease of culture, large numbers of progeny, small number of chromosomes and easily recognizable genetic traits have led to its being the most thoroughly genetically described multicellular eukaryote with thousands of mutants and chromosomal aberrations described and mapped.

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Textbooks and Manuals

  • ALBERTS, B., BRAY, D., LEWIS, J., RAFF, M., ROBERTS, K., WATSON, J.D. (eds.): Molecular Biology of the Cell, 2nd ed. New York: Garland 1989.

    Google Scholar 

  • ASHBURNER, M.: Drosophila: A Laboratory Manual. Cold Spring Harbor NY: Cold Spring Harbor Laboratory Press 1989a.

    Google Scholar 

  • ASHBURNER, M.: Drosophila: A Laboratory Handbook. Cold Spring Harbor NY: Cold Spring Harbor Laboratory Press 1989b.

    Google Scholar 

  • BOULNOIS, G.J. (ed.): Gene Cloning and Analysis: A Laboratory Guide. Oxford: Blackwell Scientific Publications 1987.

    Google Scholar 

  • BROWN, T.A.: Gene Cloning. An Introduction. Wokingham, Berks.: Van Nostrana Reinhold (UK) 1986.

    Google Scholar 

  • DE POMERAI, D.: From Gene to Animal. An Introduction to the Molecular Biology of Animal Development. Cambridge: Cambridge University Press 1985.

    Google Scholar 

  • FREIFELDER, D.: Molecular Biology, 2nd ed. Boston MA: Jones and Bartlett 1987.

    Google Scholar 

  • GLOVER, D. (ed.): DNA Cloning. A Practical Approach. Oxford: IRL Press 1985.

    Google Scholar 

  • KING, R.C.: Handbook of Genetics, vol. 3: Invertebrates of Genetic Interest. New York: Plenum Press 1975.

    Google Scholar 

  • MANIATIS, T., FRITSCH, E.F., SAMBROOK, J.: Molecular Cloning. A Laboratory Manual. Cold Spring Harbor NY: Cold Spring Harbor Laboratory Press 1982.

    Google Scholar 

  • ROBERTS, D.B. (ed.): Drosophila. A Practical Approach. Oxford: IRL Press 1986.

    Google Scholar 

  • WATSON, J.D., HOPKINS, N.H., ROBERTS, J.W., STEIRZ, J.A., WEINER, A.M.: Molecular Biology of the Gene, vols. 1 and 2. Menlo Park CA: Benjamin/Cummings Publishing Co. 1987.

    Google Scholar 

  • References cited

    Google Scholar 

  • BAKER, B.S.: Sex in flies: the splice of life. Nature 340, 521–524 (1989).

    Article  PubMed  CAS  Google Scholar 

  • BARGIELLO, T.A., YOUNG, M.W.: Molecular genetics of a biological clock in Drosophila. Proc. Nat. Acad. Sci. (USA) 81, 2142–2146 (1984).

    Article  CAS  Google Scholar 

  • BENYAJATI, C., PLACE, A.R., POWERS, D.A., SOFER, W.: Alcohol dehydrogenase gene of Drosophila melanogaster: Relationship of intervening sequences to functional domains in the protein. Proc. Nat. Acad. Sci. (USA) 78, 2717–2721 (1981).

    Article  CAS  Google Scholar 

  • BIGGIN, M.D., TJIAN, R.: Transcription factors and the control of Drosophila development. Trends Genet. 5, 377–383 (1989).

    Article  PubMed  CAS  Google Scholar 

  • BOULAY, J.L., DENNEFELD, C., ALBERGA, A.: The Drosophila developmental gene snail encodes a protein with nucleic acid binding fingers. Nature 330, 395–398 (1987).

    Article  PubMed  CAS  Google Scholar 

  • BRAY, S.J., JOHNSON, W.A., HIRSH, J., HEBERLEIN, U., TJIAN, R.: A cis-acting element and associated binding factor required for CNS expression of the Drosophila melanogaster dopa decarboxylase gene. EMBO J. 7, 177–188 (1988).

    PubMed  CAS  Google Scholar 

  • CAMPUZANO, S., CARRAMOLINO, L., CABRERA, C.V., RUIZ-GOMEZ, M., VILLARES, R., BORONAT, A., MODOLLEL, J.: Molecular genetics of the achaete-scute gene complex in Drosophila melanogaster. Cell 40, 327–338 (1985).

    Article  PubMed  CAS  Google Scholar 

  • CHOUDHARY, M., SINGH, R.S.: A comprehensive study of genie variation in natural populations of Drosophila melanogaster. III. Variations in genetic structure and their causes between Drosophila melanoaaster and its sibling species Drosophila simulans. Genetics 117, 697–710 (1987).

    PubMed  CAS  Google Scholar 

  • FJOSE, A., MCGINNIS, W.J., GEHRING, W.J.: Isolation of a homeobox-containing gene from the engrailed region of Drosophila and the spatial distribution of its transcripts. Nature 313, 284–289 (1985).

    Article  PubMed  CAS  Google Scholar 

  • GEHRING, W.J., HIROMI, Y.: Homeotic genes and the homeobox. Ann. Rev. Genet. 20, 147–173 (1986).

    Article  PubMed  CAS  Google Scholar 

  • GILMOUR, D.S., THOMAS, G.H., ELGIN, S.C.R.: Drosophila nuclear proteins bind to regions of alternating C and T residues in gene promoters. Science 245, 1487–1490 (1989).

    Article  PubMed  CAS  Google Scholar 

  • GOLDBERG, D.A.: Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene. Proc. Nat. Acad. Sci. (USA) 77, 5794–5798 (1980).

    Article  CAS  Google Scholar 

  • HOLMGREN, R., LIVAK, K., MORIMOTO, R., FREUND, R., MESELSON, M.: Studies of cloned sequences from four Drosophila heat shock loci. Cell 18, 1359–1370 (1979).

    Article  PubMed  CAS  Google Scholar 

  • KONOPKA, R.J.: Genetics of biological rhythms in Drosophila. Ann. Rev. Genet. 21, 227–236 (1987).

    Article  PubMed  CAS  Google Scholar 

  • KRISTOFFERSON, D.: The BIONET electronic network. Nature 324, 555–556 (1987).

    Article  Google Scholar 

  • KUBLI, E., SUTER, B., DOERIG, R., CHOFFAT, Y.: Suppressors, introns and stage specific expression of tRNATyr genes in Drosophila melanogaster. NATO Adv. Study Inst. Ser. H Cell Biol. 14, 235–247 (1988).

    CAS  Google Scholar 

  • LAUGHON, A., SCOTT, M.P.: Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins. Nature 310, 25–31 (1984).

    Article  PubMed  CAS  Google Scholar 

  • MARX, J.L.: Developmental control gene sequenced. Science 236, 26–27 (1989).

    Article  Google Scholar 

  • MAXAM, A.M., GILBERT, W.: A new method for sequencing DNA. Proc. Nat. Acad. Sci. (USA) 74, 560–564 (1977).

    Article  CAS  Google Scholar 

  • MCGINNIS, W., LEVINE, M.S., HAFEN, E., KUROIWA, A., GEHRING, W.J.: A conserved DNA sequence in homoeotic genes of the Drosophila melanogaster Antennapedia and bithorax complexes. Nature 308, 428–433 (1984a).

    Article  PubMed  CAS  Google Scholar 

  • MCGINNIS, W., GARBER, R.L., WIRZ, J., KUROIWA, A., GEHRING, W.J.: A homologous protein coding sequence in Drosophila homoetic genes and its conservation in other metazoans. Cell 38, 403–409 (1984b).

    Article  Google Scholar 

  • MOSES, K., ELLIS, M.C., RUBIN, G.M.: The glass gene encodes a zinc-finger protein required by Drosophila photoreceptor cells. Nature 340, 531–536 (1989).

    Article  PubMed  CAS  Google Scholar 

  • NIKI, Y., CHIGUSA, S.I., MATSUURA, E.T.: Complete replacement of mitochondrial DNA in Drosophila. Nature 341, 551–552 (1989).

    Article  PubMed  CAS  Google Scholar 

  • O’HARE, K., RUBIN, G.M.: Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell 34, 25–35 (1983).

    Article  PubMed  Google Scholar 

  • PASTINK, A., VREEKEN, C., NIVARD, M.J.M., SEARLES, L.L., VOGEL, E.W.: Sequence analysis of N-ethyl-N-nitrosourea-induced vermilion mutations in Drosophila melanogaster. Genetics 123, 123–129 (1989).

    PubMed  CAS  Google Scholar 

  • REAUME, A.G., CLARK, S.H., CHOVNICK, A.: Xanthine dehydrogenase is transported to the Drosophila eye. Genetics 123, 503–509 (1989).

    PubMed  CAS  Google Scholar 

  • REDDY, P., ZEHRING, W.A., WHEELER, D.A., PIRROTTA, V., HADFIELD, C., HALL, J.C., ROSBASH, M.: Molecular analysis of the period locus in Drosophila melanogaster and identification of a transcript involved in biological rhythms. Cell 38, 701–710 (1984).

    Article  PubMed  CAS  Google Scholar 

  • RIJSEWIJK, F., SCHUERMANN, M., WAGENAAR, E., PARREN, P., WEIGEL, D., NUSSE, R.: The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless. Cell 50, 649–658 (1987).

    Article  PubMed  CAS  Google Scholar 

  • ROSENBERG, U.B., PREISS, A., SEIFERT, E., JAECKLE, H., KNIPPLE, D.C.: Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos. Nature 313, 703–706 (1985).

    Article  PubMed  CAS  Google Scholar 

  • RUBIN, G.: Isolation of a telomeric DNA sequence from Drosophila melanogaster. Cold Spring Harbor Symp. Quant. Biol. 42, 1041–1046 (1978).

    CAS  Google Scholar 

  • RUBIN, G.M., BROREIN, W.J., JR., DUNSMUIR, P., FLAVEL, A.J., LEVIS, R., STRUBEL, E., TOOLE, J.J., YOUNG, E.: Copialike transposable elements in the Drosophila genome. Cold Spring Harbor Symp. Quant. Biol. 45, 619–628 (1981).

    CAS  Google Scholar 

  • RUBIN, G.M., SPRADLING, A.C.: Vectors for P element-mediated gene transfer in Drosophila. Nucleic Acid Res. 11, 6341–6351 (1983).

    Article  PubMed  CAS  Google Scholar 

  • SAIKI, R.K., GELFAND, D.H., STOFFEL, S., SCHARF, S.J., HIGUCHI, R., HORN, G.T., MULLIS, K.B., ERLICH, H.A.: Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–491 (1988).

    Article  PubMed  CAS  Google Scholar 

  • SANGER, F., NICKLEN, S., COULSON, A.R.: DNA sequencing with chain-terminating inhibitors. Proc. Nat. Acad. Sci. (USA) 74, 5463–54.. (1977).

    Article  CAS  Google Scholar 

  • SOUTHGATE, R., AYME, A., VOELLMY, R.: Nucleotide sequence analysis of the Drosophila small heat shock gene cluster at locus 67B. J. Mol. Biol. 165, 35–37 (1983).

    Article  PubMed  CAS  Google Scholar 

  • TAUTZ, D., HANCOCK, J.M., WEBB, D.A., TAUTZ, C., DOVER, G.A.: Complete sequences of the rRNA genes of Drosophila melanogaster. Mol. Biol. Evol. 5, 366–376 (1988).

    PubMed  CAS  Google Scholar 

  • TSUBOTA, S.I., ROSENBERG, D., SZOSTAK, H., RUBIN, D., SCHEDL, P.: The cloning of the Bar region and the B breakpoint in Drosophila melanogaster: Evidence for a transposoninduced rearrangement. Genetics 122, 881–890 (1989).

    PubMed  CAS  Google Scholar 

  • WEINTRAUB, H.M.: Antisense RNA and DNA. Sci. Amer..262(1), 34–40 (1990).

    Article  Google Scholar 

  • ZEHRING, W.A., WHEELER, D.A., REDDY, P., KONOPKA, R.J., KYRIACOU, C.P., ROSBASH, M., HALL, J.C.: P-element transformation with period locus DNA restores rhythmicity to mutant, arrhythmic Drosophila melanogaster. Cell 39. 369–376 (1984).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Toxikology, ETH and University of Zurich, Schorenstreet 16, CH-8603, Schwerzenbach, Switzerland

    Dr. sc. nat. Ulrich Graf & Prof. Dr. sc. nat. Friedrich E. Würgler

  2. Department of Genetics, University of the Witwatersrand, 1 Jan Smuts Ave., P.O. Wits, 2050, Johannesburg, South Africa

    Prof. Ph. D. Nancy van Schaik

Authors
  1. Dr. sc. nat. Ulrich Graf
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  2. Prof. Ph. D. Nancy van Schaik
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  3. Prof. Dr. sc. nat. Friedrich E. Würgler
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© 1992 Springer-Verlag Berlin Heidelberg

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Graf, U., van Schaik, N., Würgler, F.E. (1992). Molecular Biology. In: Drosophila Genetics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76805-7_8

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  • DOI: https://doi.org/10.1007/978-3-642-76805-7_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-54327-5

  • Online ISBN: 978-3-642-76805-7

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