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Lectins and Cancer pp 27–40Cite as

Narcissus and Gerardia lectins: Tools for the development of a vaccine against AIDS and a new ELISA to quantify HIV-gp 120

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Abstract

The lectins, isolated from Narcissus pseudonarcissus [NPL] and Gerardia savaglia [Gerardia-lectin], are specific for terminal Man(α1-3)Man units. They react very specifically with the external glycoprotein gp120 of the AIDS-virus, the human immunodeficiency virus type 1 (HIV-1). They prevent in vitro the infection of cells with HIV. These lectins were tools for identifying antigens [mannans from Candida albicans and from Saccharomyces cerevisiae, comprising Man(α1-3)Man and Man(α1-2)Man linked residues] which were successfully used to raise in rabbits neutralizing antibodies against HIV infection in vitro. The NPL was used to develop a novel enzyme-linked immunoassay [ELISA] system for quantifying HIV-gp120 within the range of 0.6 to 20,000 ng/ml.

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Authors and Affiliations

  1. Abteilung “Angewandte Molekularbiologie”, Universität, Institut für Physiologische Chemie, Duesbergweg 6, D-6500, Mainz, Germany

    W. E. G. Müller, J. M. S. Forrest, S.-H. Chang, B. E. Weiler, H. Schäcke, E. Matthes & M. Bachmann

  2. Scottish Crop Research Institute, Invergowrie, Dundee, UK

    J. M. S. Forrest

  3. Zentralinstitut für Molekularbiologie, D-1115, Buch, Berlin, Germany

    E. Matthes

Authors
  1. W. E. G. Müller
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  2. J. M. S. Forrest
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  3. S.-H. Chang
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  4. B. E. Weiler
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  5. H. Schäcke
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  6. E. Matthes
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  7. M. Bachmann
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Editors and Affiliations

  1. Max-Planck-Institut für experimentelle Medizin, Hermann-Rein-Straße 3, 3400, Göttingen, Germany

    Hans Joachim Gabius

  2. Abteilung Hämatologie/Onkologie, Medizinische Universitätsklinik, Robert-Koch-Straße 40, 3400, Göttingen, Germany

    Sigrun Gabius

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© 1991 Springer-Verlag Berlin Heidelberg

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Müller, W.E.G. et al. (1991). Narcissus and Gerardia lectins: Tools for the development of a vaccine against AIDS and a new ELISA to quantify HIV-gp 120. In: Gabius, H.J., Gabius, S. (eds) Lectins and Cancer. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76739-5_3

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  • DOI: https://doi.org/10.1007/978-3-642-76739-5_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76741-8

  • Online ISBN: 978-3-642-76739-5

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