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Genetic Analysis of Heat Shock Protein Functions in Yeast

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Heat Shock

Abstract

My laboratory has investigated the function of heat-shock proteins in Saccharomyces cerevisiae by cloning the genes encoding these proteins, creating mutations in the cloned genes by in vitro disruption mutagenesis, and replacingwild-typegeneswith the mutated versions by gene conversion in vivo (Rothstein, 1983). Here I will briefly review the results obtained with three genes, those encoding HSP26, HSP82, and HSP 104 (Fig. 1).

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References

  • Borkovich, KA, Farrelly, FW, Finkelstein, DB, Taulien, J and Lindquist, S, (1989) hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures. Mol. Cell Biol., 9: 3919–3930.

    PubMed  CAS  Google Scholar 

  • Brugge, J, Yonemoto, W ana Darrow, D, (1983) Interaction between the Rous sarcoma virus transferring protein and two cellular phosphoproteins: Analysis of the time-over and distribution of this complex. Mol. Cell. Biol., 3: 9–19.

    PubMed  CAS  Google Scholar 

  • Courtneidge, SA and Bishop, JM, (1982) Transit of pp60v-src to the plasma membrane. Proc. Natl. Acad. Sci. USA, 79: 7117–7121.

    Article  CAS  Google Scholar 

  • Catelli, MG, Binart, N, Jung-Testas, I, Renoir, J-M, Baulieu, E-E, etal., (1985) Hie common 90Kd protein component of non-transformed ‘85’ steroid receptors is a heat shock protein. EMBO J., 4: 3131–3135.

    PubMed  CAS  Google Scholar 

  • Ingolia, TD and Craig, EA, (1982) Four small Drosophila heat shock proteins are related to each other and to mammalian a-eristallin. Proc. Natl. Acad. Sci. USA, 79: 2360–2364.

    Article  CAS  Google Scholar 

  • Lindquist, S, (1986) The heat-shock response. Ann. Rev. Biochem., 55: 1151–1191.

    Article  PubMed  CAS  Google Scholar 

  • Lindquist, S and Craig, EA, (1988) Hie heat shock proteins. Annu. Rev. Genet., 22: 631–677.

    Article  PubMed  CAS  Google Scholar 

  • Petko, L and Lindquist, S, (1986) Hsp26 is not required for growth at high temperatures, northe thennotolerance, spore development, or germination. Cell, 45: 885–894.

    Article  PubMed  CAS  Google Scholar 

  • Picard, D, Khursheed, B, Garabedian, MJ, Fortin, MG, Lindquist, S and Yamamoto, KR, (1990) Reduced levels of hsp90 compromise steroid receptor action in vivo. Nature, 348: 166–168.

    Article  PubMed  CAS  Google Scholar 

  • Rossi, JM and Lindquist, SL, (1989) The intracellular location of yeast HSP26 varies with metabolism. J. Cell Biol., 108: 425–439.

    Article  PubMed  CAS  Google Scholar 

  • Rothstein, RJ, (1983) One-step gene disruption in yeast. Methods Enzymol., 101: 201–211.

    Google Scholar 

  • Sanchez, ER, Toft, DO, Schlessinger, MJ and Pratt, WB, (1985) Evidence that the 90-kda phosphoprotein associated with the untrasformed c-eell glucocorticoid is a murine heat shock protein. c., 260: 12398–12401.

    PubMed  CAS  Google Scholar 

  • Sanchez, Y and Lindquist, S, (1990) HSP104 required for induced thermotolerance. Science, 248: 1112–1115.

    Article  PubMed  CAS  Google Scholar 

  • Susek, RE and Lindquist, SL, (1980) hsp26 of Saccharomyces cerevisiae is related to the superfamily of small heat shock proteins but is without a demonstrable function. Mol. Cell Biol., 9: 5265–5271.

    Google Scholar 

  • Wejksnora, PJ and Haber, JE, (1978) Ribonueleoprotein particle appearing during sporulation in yeast. J. Baeteriol., 134: 246–260.

    CAS  Google Scholar 

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© 1991 Springer-Verlag Berlin Heidelberg

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Lindquist, S. (1991). Genetic Analysis of Heat Shock Protein Functions in Yeast. In: Maresca, B., Lindquist, S. (eds) Heat Shock. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76679-4_14

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  • DOI: https://doi.org/10.1007/978-3-642-76679-4_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76681-7

  • Online ISBN: 978-3-642-76679-4

  • eBook Packages: Springer Book Archive

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