Racial Differences in the Expression of Shared Idiotypes by Paraproteins from Patients with Monoclonal Gammopathies
Twenty-nine murine monoclonal antibodies have been produced which react with shared idiotypes expressed by B-cell lymphomas and leukemias. We tested this panel of antibodies for reactivity with the paraproteins from 37 patients with multiple myeloma and 13 patients with monoclonal gammopathy of undetermined significance (MGUS). Fourteen of 50 paraproteins reacted with at least one antibody in this panel of anti-idiotypic antibodies. Seven different anti-idiotypes demonstrated reactivity with the paraproteins. A similar frequency of reactivity was found for both myeloma and MGUS proteins. One antibody, S30–47, reacted with eight (16%) of the paraproteins from patients with these monoclonal gammopathies; whereas, this anti-idiotype only bound to 3% of non-Hodgkin’s B-cell lymphomas and no cases of chronic lymphocytic leukemia. This antibody demonstrated reactivity with paraproteins obtained from nearly one-third of black patients but none of the white patients’ paraproteins showed reactivity with this antiidiotype. In each of eleven patients tested, plasma cells isolated from bone marrow were shown to be reactive with the same anti-idiotype we found to react with the paraprotein. Anti-shared idiotype antibodies may provide useful reagents for studies of patients with monoclonal gammopathies.
KeywordsMultiple Myeloma Chronic Lymphocytic Leukemia Bone Marrow Plasma Cell Idiotypic Determinant Chain Variable Region Gene
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