Paternity Analysis in a Deficient Family by DNA Profiling (Single-Locus and Multi-Locus Probes)

  • E. Iten
  • R. Pflugshaupt

Abstract

With classical analytical methods without HLA typing, cases of disputed paternity are problematic when the putative father is not available for analysis. Due to the small number of distinguishable alleles per system, exclusions from paternity are relatively rare. Moreover, they have to be taken with care, since the assumed descent of the putative father may be incorrect When only one paternal grandparent is available for analysis, it is impossible to derive any exclusion. HLA analysis improves the probability of exclusion, but the number of analysable loci is limited and the loci are linked.

Keywords

EDTA Agarose Citrate Electrophoresis Nylon 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Smith JC, Anwar R, Riley J, Jenner D, Markham AF (1990) Highly polymorphic minisatellite sequences: allele frequencies and mutation rates for five locus-specific probes in a Caucasian population. J Forensic Sci Soc 30:19–32PubMedCrossRefGoogle Scholar
  2. 2.
    Jeffreys AJ, Wilson V, Thein SL (1985) Hypervariable ‘minisatellite’ regions in human DNA. Nature 314:67–73PubMedCrossRefGoogle Scholar
  3. 3.
    Jeffreys AJ, Wilson V, Thein SL (1985) Individual-specific ‘fingerprints’ of human DNA. Nature 316:76–79PubMedCrossRefGoogle Scholar
  4. 4.
    Schacker U, Schneider PM, Holtkamp B, Bohnke E, Fimmers R, Sonnebom HH, Rittner C (1990) Isolation of the DNA minisatellite probe MZ 1.3 and its application to DNA ‘fingerprinting’ analysis. Forensic Sci Int 44:209–224PubMedCrossRefGoogle Scholar
  5. 5.
    Gertdler SJ, Burchell JM, Duhig T, Lamport D, White R, Parker M, Taylor-Papadimitriou J (1987) Cloning of partial cDNA encoding differentiation and tumor-associated mucin glycoprotein expressed by human mammary epithelium. Proc Natl Acad Sci USA 84:6060–6064CrossRefGoogle Scholar
  6. 6.
    Dykes DD (1988) The use of biotinylated DNA probes in parentage testing: Non-isotopic labelling and non-toxic extraction. Electrophoresis 9:359–368PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • E. Iten
  • R. Pflugshaupt
    • 1
  1. 1.Central LaboratoryBlood Transfusion Service SRCBern 22Switzerland

Personalised recommendations