A Proposed Combination of Single Locus Probes for Multiple Rehybridization
Parallel investigations have been carried out on a population from the Münster area using the probes 355–8, 336 and 427–4 and the restriction enzymes Hinf I and Pst I. The distribution frequencies with the probes 336 and 427–4 were clustered in the 2 kb region with additional isolated fragments up to the 10 kb area. A comparison between the Hinf I and Pst I digests showed a close similarity of the frequency distributions for 336 and 427–4 but a clear shift was found in the fragment size range exclusively with 355–8. The fragments for Pst I digest were approx 4 kb higher than the corresponding Hinf I results.
The combined spread of the 3 polymorphisms using Hinf I ranged from 1–10 kb. A comparison between the distribution ranges of Münster and America showed a conspicuously wider distribution of pl 336 in the American survey and the contrary using 427–4. A heterozygosity rate greater than 90% for the 3 polymorphisms after Hinf I digest provides efficient biostatistical evidential value.
KeywordsEDTA Agarose Electrophoresis Nylon Formamide
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