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A Proposed Combination of Single Locus Probes for Multiple Rehybridization

  • P. Wiegand
  • S. Rand
  • B. Brinkmann

Summary

Parallel investigations have been carried out on a population from the Münster area using the probes 355–8, 336 and 427–4 and the restriction enzymes Hinf I and Pst I. The distribution frequencies with the probes 336 and 427–4 were clustered in the 2 kb region with additional isolated fragments up to the 10 kb area. A comparison between the Hinf I and Pst I digests showed a close similarity of the frequency distributions for 336 and 427–4 but a clear shift was found in the fragment size range exclusively with 355–8. The fragments for Pst I digest were approx 4 kb higher than the corresponding Hinf I results.

The combined spread of the 3 polymorphisms using Hinf I ranged from 1–10 kb. A comparison between the distribution ranges of Münster and America showed a conspicuously wider distribution of pl 336 in the American survey and the contrary using 427–4. A heterozygosity rate greater than 90% for the 3 polymorphisms after Hinf I digest provides efficient biostatistical evidential value.

Keywords

Parallel Investigation Fragment Distribution High Molecular Weight Region Molecular Range Heterozygosity Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • P. Wiegand
  • S. Rand
  • B. Brinkmann
    • 1
  1. 1.Institut für RechtsmedizinWestfälischen Wilhelms-UniversitätMünsterGermany

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