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Isolierung und Analytik von Polysacchariden

  • W. Blaschek
Chapter

Zusammenfassung

Das Kapitel befaßt sich mit der Isolierung und Strukturaufklärung von Polysacchariden. Da davon ausgegangen wird, daß der Leser hauptsächlich an grundlegenden Methoden in diesem Arbeitsbereich interessiert ist, soll in diesem Kapitel weiterführende Literatur zu einzelnen Arbeitstechniken und Methoden aufgelistet werden, die entweder direkte Arbeitsvorschriften beinhaltet oder aber Übersichtsartikel darstellt, aus denen spezifische Publikationen entnommen werden können.

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Literatur

  1. Adams GA (1965) Complete acid hydrolysis. In: Whistler RL (ed) Methods in Carbohydrate Chemistry, Vol. 5. Academic Press, New York, pp 269–275 u. 285–287Google Scholar
  2. Aspinall GO (1976) Polysaccharide methodology. In: Aspinall GO (ed) Organic Chemistry, Series Two, Vol. 7, Carbohydrates. Butterworths, London, pp 201–222Google Scholar
  3. Aspinall GO, (1982a) Isolation and Fractionation of Polysaccharides. In: Aspinall GO (ed) The Polysaccharides, Vol. 1. Academic Press, New York, pp 19–34Google Scholar
  4. Aspinall GO (1982b) Chemical Characterization and structure determination of polysaccharides. In: Aspinall GO (ed) The Polysaccharides, Vol. 1. Academic Press, New York, pp 35–131Google Scholar
  5. Aspinall GO, Molloy JA, Craig JWT (1969) Extracellular polysaccharides from suspension-cultured sycamore cells. Can J Biochem 47: 1063–1070PubMedCrossRefGoogle Scholar
  6. Barker R, Nunez HA, Rosevear P, Serianni AS (1982) 13C NMR Analysis of Complex Carbohydrates. Meth Enzymol 83, pp 58–69PubMedCrossRefGoogle Scholar
  7. Biermann J, McGinnis GD (1989) Analysis of Carbohydrates by GLC and MS. CRC Press, Boca Raton Binkley (1965) Dialysis. In: Whistler RL, BeMiller JN, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol. V. Academic Press, New York, pp 54–55Google Scholar
  8. Björndal H, Hellerquvist CG, Lindberg B, Svensson S (1970) Gas-Flüssigkeits-Chromatographie und Massenspektrometrie bei der Methylierungsanalyse von Polysacchariden. Angew Chem 16: 643–674CrossRefGoogle Scholar
  9. Björndal H, Lindberg B, Svensson S (1967) Mass spectrometry of partially methylated alditol acetates. Carbohydr Res 5: 433–440CrossRefGoogle Scholar
  10. Blakeney AB, Harris PJ, Stone BA (1983) A simple and rapid preparation of alditol acetates for monosaccharide analysis. Carbohydr Res 113:291-CrossRefGoogle Scholar
  11. Blakeney AB, Stone BA (1985) Methylation of carbohydrates with lithium methylsulphinyl carbanion. Carbohydr Res 140: 319–324CrossRefGoogle Scholar
  12. Blaschek W (1983) Complete separation and quantification of neutral sugars from plant cell walls and mucilages by high performance liquid chromatography. J Chromatogr 256: 157–163CrossRefGoogle Scholar
  13. Blaschek W., Koehler H., Semler U., Franz G. (1982) Molecular weight distribution of cellulose in primary cell walls. Investigations with regenerating protoplasts, suspension cultured cells and mesophyll of tobacco. Planta 154: 550–555CrossRefGoogle Scholar
  14. Blumenkrantz N, Asboe-Hansen G (1973) New method for quantitative determination of uronic acids. Anal Biochem 54: 484–489PubMedCrossRefGoogle Scholar
  15. Bouveng HO, Lindberg B (1965) Native acetylated wood polysaccharides. Extraction with dimethyl sulfoxide. In: Whistler RL (ed) Methods in Carbohydrate Chemistry, Vol. 5. Academic Press, New York, pp 147–150Google Scholar
  16. Bradbury AGW, Halliday DJ, Medcalf DG (1981) Separation of monosaccharides as trimethylsilylated alditols on fused-silica capillary columns. J Chromatogr 213: 146–150CrossRefGoogle Scholar
  17. Brett CT (1981) Polysaccharide synthesis from GDP-glucose in pea epicotyl slices. J Exp Bot 32: 1067–1077CrossRefGoogle Scholar
  18. Brobst KM (1972) Gas-Liquid Chromatography of Trimethylsilyl Derivatives. In: Whistler RL, BeMiller JN (eds) Methods in Carbohydrate Chemistry, Vol. VI. Academic Press, New York, pp 3–8Google Scholar
  19. Carpita NC (1986) Incorporation of proline and amino acids into the cell walls of maize coleoptiles. Plant Physiol 80: 660–666PubMedCrossRefGoogle Scholar
  20. Chanzy H, Chumpitazi B, Peguy A (1982) Solutions of polysaccharides in N-methyl morpholine N-oxide ( MMNO ). Carbohydr Polym 2: 35–42CrossRefGoogle Scholar
  21. Churms SC (1970) Gel chromatography of carbohydrates. In: Tipson RS, Horton D (eds) Advances in Carbohydrate Chemistry and Biochemsitry, Vol 25. Academic Press, New York, p 13–26Google Scholar
  22. Ciucanu I, Kerek F (1984) A simple and rapid method for the permethylation of carbohydrates. Carbohydr Res 131: 209–217CrossRefGoogle Scholar
  23. Cooper TG (1981) Biochemische Arbeitsmethoden. Walter de Gruyter, BerlinGoogle Scholar
  24. DeBelder AN, Normann B (1968) The distribution of substituents in partially acetylated Dextran. Carbohydr Res 8: 1–6CrossRefGoogle Scholar
  25. DeJongh DC (1980) Mass Spectrometry. In: Pigman W, Horton D, Wander J (eds) The Carbohydrates: Chemistry and Biochemistry, Vol. IB. Academic Press, New York, pp 1327–1353Google Scholar
  26. Dische Z (1962) Color reactions of carbohydrates. In: Whistler RL, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol. 1. Academic Press, New York, pp 475–514Google Scholar
  27. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350–356CrossRefGoogle Scholar
  28. Fry CF (1988) The growing plant cell wall: chemical and metabolic analysis. Longman Scientific and Technical, EssexGoogle Scholar
  29. Garcia-Raso A, Martinez-Castro I, Paez ML, Sanz J, Garda-Raso J, Saura-Calixto F (1987) Gas chromatographic behaviour of carbohydrate trimethylsilyl ethers. I. Aldopentoses. J chromatogr 398:9-CrossRefGoogle Scholar
  30. Goldstein IJ, Hay GW, Lewis BA, Smith F (1965) Controlled Degradation of Polysaccharides by Periodate Oxidation, Reduction, and Hydrolysis. In: Whistler RL, BeMiller JN, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol. V. Academic Press, New York, pp 361–370Google Scholar
  31. Gorin PA (1981) Carbon-13 nuclear magnetic resonance spectroskopy of polysaccharides. Adv Carbohydr Chem Biochem 38: 13–104CrossRefGoogle Scholar
  32. Guerrant GO, Moss CW (1984) Determination of monosaccharides as aldononitrile, O-methyloxime, alditol, and cyclitol acetate derivatives by gas chromatography. Anal. Chem 56: 633–638CrossRefGoogle Scholar
  33. Hais IM, Macek K (eds) (1963) Paper Chromatography, a Comprehensive Treatise, 3rd edn. Academic Press, New YorkGoogle Scholar
  34. Hakamori S (1964) A rapid permethylation of glycolipid, and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide. J Biochem 55: 205–208Google Scholar
  35. Hall LD (1980) High-Resolution NMR Spectroscopy. In: Pigman W, Horton D, Wander J (eds) The carbohydrates: Chemistry and Biochemistry, Vol. IB. Academic Press, New York, pp 1299–1326Google Scholar
  36. Hay GW, Lewis BA, Smith F (1965) Periodate Oxidation of Polysaccharides: General Procedures. In: Whistler RL, BeMiller JN, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol. V. Academic Press, New York, pp 357–361Google Scholar
  37. Harris PJ (1983) Cell Walls. In: Hall JL, Moore AL (eds) Isolation of membranes and organelles from plant cells. Academic Press, London, pp 25–53Google Scholar
  38. Harris PJ, Henry RJ, Blakeney AB, Stone BA (1984) An improved procedure for the methylation analysis of oligosaccharides and polysaccharides. Carbohydr Res 127: 59–73PubMedCrossRefGoogle Scholar
  39. Harris PJ, Bacic A, Clarke AE (1985) Capillary gas chromatography of partially methylated alditol acetates on a SP-21oo wall-coated open-tubular column. J Chromatogr 350:304-CrossRefGoogle Scholar
  40. Hicks KB, Lim PC, Haas MJ (1985) Analysis of uronic acids, their lactones, and related compounds by high performance liquid chromatography on cation-exchange resins. J Chromatogr 319: 159–171CrossRefGoogle Scholar
  41. Hon S, Srinivasan KSV (1983) Mechanochemical process in cotton cellulose fiber. J appl Polym Sci 28: 1–10CrossRefGoogle Scholar
  42. Jansson P-E, Kenne L, Liedgren H, Lindberg B, Lonngren J (1976) A Practical Guide to the Methylation Analysis of Carbohydrates. Chem Comm. 8: 2–74Google Scholar
  43. Jarvis MC (1982) The proportion of calcium-bound pectin in Plant cell walls. Planta 154: 344–346CrossRefGoogle Scholar
  44. Jennings HJ, Smith ICP (1980) Determination of Polysaccharide Structures with 13C-NMR. In: Whistler RL, BeMiller JN (eds) Methods in Carbohydrate Chemistry, Vol. VIII. Academic Press, New York, pp 97–105Google Scholar
  45. John M, Schmidt J, Wandrey C, Sahm H (1982) Gel Chromatography of Oligosaccharides up to DB 60. J Chromatogr 247: 281–288CrossRefGoogle Scholar
  46. Jork H, Funk W, Fischer W, Wimmer H (1989) Dünnschicht-Chromatographie: Reagenzien und Nachweismethoden. VCH, WeinheimGoogle Scholar
  47. Joseleau J-P, Chambat G, Chumpitazi-Hermoza B (1980) Solubilization of cellulose and other plant structural polysaccharides in 4-methylmorpholine-N-oxide: an improved method for the study of cell wall constituents. Carbohydr Res 90: 339–344CrossRefGoogle Scholar
  48. Jones JKN, Stoodley RJ (1965) Fractionation by Ultrafiltration. In: Whistler RL, BeMiller JN, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol. V. Academic Press, New York, pp 47–48Google Scholar
  49. Jones JKN, Stoodley RJ (1965) Fractionation using copper complexes. In: Whistler RL (ed) Methods in Carbohydrate Chemistry, Vol. 5. Academic Press, New York, pp 36–38Google Scholar
  50. Klok J, Nieberg-Van Velzen EH, De Leeuw JW, Schenck PA (1981) Capillary gas chromatographic separation of monosaccharides as their alditol acetates. J Chrom. 207: 273–275CrossRefGoogle Scholar
  51. Kobota A, Yamashita K, Takasaki S (1987) Biogel P-4 Column chromatography of oligosaccharides: effective size of oligosaccharides expressed in glucose units. Meth Enzymol 138: 84–94CrossRefGoogle Scholar
  52. Kochetkov NK, Chizhov OS (1966) MS of carbohydrate derivatives. Adv Carbohydr Chem 21: 185–240Google Scholar
  53. Layne E (1957) Spectrophotometry and turbidometric methods for measuring proteins. Meth Enzymol 3: 447–454CrossRefGoogle Scholar
  54. Lecacheux D (1988) Preparative Fractionation of Natural Polysaccharides by Size Exclusion Chromatography. Carbohydr Polymers 8: 119–130CrossRefGoogle Scholar
  55. Lee YC, Montgomery R (1965) Separations with Molecular Sieves. In: Whistler EL, BeMiller JN, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol. V. Academic Press, New York, 28–34Google Scholar
  56. Lemieux RU, Stevens JD (1966) The proton magnetic resonance spectra and tautomeric equilibria of aldoses in deuterium oxide. Canad J Chem 44: 249–262CrossRefGoogle Scholar
  57. Lever M (1972) A new reaction for colorimetric determination of carbohydrates. Anal Biochem 47: 273–279PubMedCrossRefGoogle Scholar
  58. Lindberg B, Lonngren J, Svensson S (1975) Specific degradation of polysaccharides. Adv Carbohydr Chem Biochem 31: 185–241CrossRefGoogle Scholar
  59. Lindberg B, Lonngren J (1978) Methylation analysis of complex carbohydrates: general procedure and application of sequence analysis. Meth Enzym 50: 3–32PubMedCrossRefGoogle Scholar
  60. Loewus F, Shah RH (1972) Gas-Liquid Chromatography of Trimethylsilyl Ethers of Cyclitols. In: Whistler RL, BeMiller JN (eds) Methods in Carbohydrate Chemistry, Vol. VI. Academic Press, New York, pp 14–20Google Scholar
  61. Lomax JA, Conchie J (1982) Separation of methylated alditol acetates by glass capillary gas chromatography and their identification by computer. J Chrom 236: 385–394CrossRefGoogle Scholar
  62. Lomax JA, Gordon AH, Chesson A (1983) Methylation of unfractionated, primary and secondary cell-walls of plants, and the location of alkali-labile substituents. Carbohydr Res 122: 11–22CrossRefGoogle Scholar
  63. McGinnis GD, Fang P (1980) High performance Liquid Chromatography. In: Whistler RL, BeMiller JN (eds) Methods in Carbohydrate Chemistry, Vol. VIII. Academic Press, New York, pp 33–43Google Scholar
  64. McNeil M, Darvill AG, Aman AG, Franzen L-E, Albersheim P (1982) Structural analysis of complex carbohydrates using high-performance liquid chromatography, gas chromatography, and mass spectrometry. Meth Enzymol 83: 3–45PubMedCrossRefGoogle Scholar
  65. Meier H (1965) Fractionation by precipitation with barium hydroxide. In: Whistler RL (ed) Methods in Carbohydrate Chemistry, Vol. 5. Academic Press, New York, pp 45–46Google Scholar
  66. Olaitan SA, Northcote DH (1962) Polysaccharides of Chlorella pyrenoidosa. Biochem J 82: 509–519PubMedGoogle Scholar
  67. Oshima R, Yoshikawa A, Kumanotani JU (1981) High-resolution gas chromatographic separation of alditol acetates on fused-silica wall-coated open-tubular columns. J Chromatogr 213: 142–145CrossRefGoogle Scholar
  68. Painter TJ (1965) Partial Acidic and Enzymic Hydrolysis. In: Whistler RL, BeMiller JN, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol. V. Academic Press, New York, pp 280–285Google Scholar
  69. Pecina R, Bonn G, Burtscher E, Bobleter O (1984) High performance liquid chromatographic elution behaviour of alcohols, aldehydes, ketones, organic acids and carbohydrates on a strong cation-exchange stationary phase. J. Chromatogr 287: 245–258CrossRefGoogle Scholar
  70. Perlin AS (1976) Carbon-13 NMR-spectroscopy of carbohydrates. In: Aspinall GO (ed) Organic Chemistry, Series Two, Vol. 7, Carbohydrates. Butterworths, London, 1–34Google Scholar
  71. Perlin AS, Casu B (1982) Spectroscopic Methods. In: Aspinall GO (ed) The Polysaccharides, Vol. 1. Academic Press, New York, pp l33–193Google Scholar
  72. Perry MB, Hulyalkar RK (1965) The analysis of hexuronic acids in biological materials by gas-liquid partition chromatography. Can J Biochem 43: 573–584PubMedCrossRefGoogle Scholar
  73. Pharmacia Fine Chemicals: Gel Filtration: Theory and Practice. FinnenschriftGoogle Scholar
  74. Phillips LR, Fräser BA (1981) Methylation of carbohydrates with dimsyi potassium in dimethyl sulfoxide. Carbohydr Res 90: 149–152CrossRefGoogle Scholar
  75. Pittet AO (1965) Dissolution of polysaccharides. In: Whistler RL, BeMiller JN, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol. V. Academic Press, New York, pp 3–5Google Scholar
  76. Read SM, Northcote DH (1981) Minimization of variation in the response to different proteins of the Comassie Blue G dye-binding assay for protein. Anal Biochem 116: 53–64PubMedCrossRefGoogle Scholar
  77. Rocklin RD, Pohl CA (1983) Determination of carbohydrates by anion exchange chromatography with pulsed amperometric detection. J Liqu Chromatogr 6: 1577–1590CrossRefGoogle Scholar
  78. Scott JE (1965) Fractionation by Precipitation with Quarternary Ammonium Salts. In: Whistler RL, BeMiller JN, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol. V. Academic Press, New York, pp 38–44Google Scholar
  79. Selvendran RR, O’Neill MA (1987) Isolation and analysis of cell walls from plant material- In: Glick D (ed) Methods of Biochemical Analysis, Vol. 32. Wiley, pp 25–153CrossRefGoogle Scholar
  80. Selvendran RR, Stevens BJH, O’Neill MA (1985) Developments in the isolation and analysis of cell walls from edible plants. In: Brett CT, Hillman JR (eds) Biochemistry of plant cell walls. Cambridge University Press, pp 39–78Google Scholar
  81. Sherma J, Shirley CC (eds) (1991) CRC Handbook of Chromatography: Carbohydrates, Vol. DL CRC- Press, Boca RatonGoogle Scholar
  82. Sloneker JH (1972) Gas-Liquid Chromatography of Alditol Acetates. In: Whistler RL, BeMiller JN (eds) Methods in Carbohydrate Chemistry, Vol. VI. Academic Press, New York, pp 20–24Google Scholar
  83. Smith I (ed) (1960) Chromatographic and Electrophoretic Techniques, Vol. 1, Chromatography, 2nd edn. Heinemann, LondonGoogle Scholar
  84. Stahl E (ed) (1967) Dünnschicht-Chromatographie: Ein Laboratoriumshandbuch, 2nd edn. Springer, New YorkGoogle Scholar
  85. Sweeley CC, Nunez HA (1985) Structural analysis of glycoconjugates by MS and NMR-spectroscopy. Ann Rev Biochem 54: 765–801PubMedCrossRefGoogle Scholar
  86. Taylor RL, Conrad HE (1972) Stoichiometric Depolymerization of Polyuronides and Glycosaminoglycuronans to Monosaccharides following Reduction of their Carbodiimide-Activated Carboxyl Groups. Biochem 11: 1383–1388CrossRefGoogle Scholar
  87. Thornber JP, Northcote DH (1962) Changes in the chemical composition of a cambial cell during its differentiation into xylem and phloem tissues in trees. 3. Xylan, glucomannan and cellulose fractions. Biochem J 82: 340–346PubMedGoogle Scholar
  88. Touchstone JC, Dobbins MF (1983) Practice of thin layer chromatography, 2nd edn. Wiley, New YorkGoogle Scholar
  89. Updegraff DM (1969) Semi-micro determination of cellulose in biological materials. Anal Biochem 32: 420–424PubMedCrossRefGoogle Scholar
  90. Valent BS, Darvill AG, McNeil M, Robertson BK, Albersheim P (1980) A general and sensitive chemical method for sequencing the glycosyi residues of complex carbohydrates. Carbohydr Res 79: 165–192PubMedCrossRefGoogle Scholar
  91. Verhaar LAT, Küster BFM (1981) Liquid chromatography of sugars on silica-based stationary phases. J. Chromatogr 220: 313–328PubMedCrossRefGoogle Scholar
  92. Waeghe TJ, Darvill AG, McNeil M, Albersheim P (1983) Blakeney u. Stone (1985) Determination, by methylation analysis, of the glycosyl-linkage compositions of microgram quantities of complex carbohydrates. Carbohydr Res 123: 281–304CrossRefGoogle Scholar
  93. Wells GB, Kontoyiannidou V, Turco SJ, Lester RL (1982) Resolution of acetylated oligosaccharides by reverse-phase high pressure liquid chromatography. Meth Enzymol 83: 132–137PubMedCrossRefGoogle Scholar
  94. Whistler RL, Anisuzzaman AKM (1980) Gel Permeation chromatography. In: Whistler RL, BeMiller JN(eds) Methods in Carbohydrate Chemistry, Vol. VUL Academic Press, New York, pp 45–53Google Scholar
  95. Whistler RL, Feather MS (1965) Hemicellulose. Extraction from annnal plants with alkaline solution. In: Whistler RL (ed) Methods in Carbohydrate Chemistry, Vol. 5. Academic Press, New York, pp 144–145Google Scholar
  96. Whistler RL, Sannella JL (1965) Fractional Precipitation with Ethanol In: Whistler RL, BeMiller JN, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol. V. Academic Press, New York, pp 34–36Google Scholar
  97. Wing RE (1972) Thin-Layer Chromatography. In: Whistler RL, BeMiller JN (eds) Methods in Carbohydrate Chemistry, Vol. VI. Academic Press, New York, pp 42–53; 54–59; 60–64Google Scholar
  98. Wolfrom ML, Franks NE (1965) Partial Acid Hydrolysis. In: Whistler RL, BeMiller JN, Wolfrom ML (eds) Methods in Carbohydrate Chemistry, Vol V. Academic Press, New York, pp 276–280Google Scholar
  99. Yamasita K, Mizuochi T, Kobata A (1982) Analysis of oligosaccharides by gel filtration. Meth Enzymol 83: 105–126CrossRefGoogle Scholar
  100. Zweig G, Sherma J, Churms SC (eds) (1982) CRC Handbook of Chromatography: Carbohydrates, Vol. I. CRC-Press, Boca RatonGoogle Scholar

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  • W. Blaschek

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