Phylogenetic Identification of Uncultivated Microorganisms in Natural Habitats
The development of oligodeoxynucleotide probes for rapid microbial identification generally requires laboratory cultivation of the organism to be detected so that gene sequences can be determined. However, microbiologists generally agree that at most 0.1 to 10% of microscopically observed organisms in nature can be grown axenically in the laboratory (see Atlas and Bartha 1981). The inability to culture a majority of microorganisms undoubtedly has precluded the discovery of many novel organisms, including medically important ones. Moreover, even if a pure culture is established, the question often remains as to whether the organism in culture is relevant to the study, or simply is the most readily cultivated.
KeywordsEDTA Electrophoresis Fractionation Bacillus Polyacrylamide
Unable to display preview. Download preview PDF.
- Atlas RM, Bartha R (1981) Microbial ecology. Addison-Wesley, PhilippinesGoogle Scholar
- DeLong EF, Schmidt TM, Pace NR (1990) Analysis of single cells and oligotrophic picoplankton populations using 16S rRNA sequences. In: Hattori T, Ishida Y, Maruyama Y, Morita RY, Uchida A (eds) Proc 5th Int Symp Microbial ecology. Japan Scientific Societies Press, TokyoGoogle Scholar
- Pace NR, Stahl DA, Lane DJ, Olsen GJ (1986) The analysis of natural microbial populations by ribosomal RNA sequences. Adv Microb Ecol 9: 1–55Google Scholar
- Stahl DA, Lane DJ, Olsen GJ, Pace NR (1985) Characterization of a Yellowstone hot spring microbial community by 5S rRNA sequences. Appl Environ Microbiol 45: 1379–1384Google Scholar
- Weller R, Ward DM (1989) Selective recovery of 16S rRNA sequences from natural microbial communities in the form of cDNA. Appl Environ Mirobiol 55: 1818–1822Google Scholar