Phylogenetic Identification of Uncultivated Microorganisms in Natural Habitats

  • T. M. Schmidt
  • E. F. Delong
  • N. R. Pace

Abstract

The development of oligodeoxynucleotide probes for rapid microbial identification generally requires laboratory cultivation of the organism to be detected so that gene sequences can be determined. However, microbiologists generally agree that at most 0.1 to 10% of microscopically observed organisms in nature can be grown axenically in the laboratory (see Atlas and Bartha 1981). The inability to culture a majority of microorganisms undoubtedly has precluded the discovery of many novel organisms, including medically important ones. Moreover, even if a pure culture is established, the question often remains as to whether the organism in culture is relevant to the study, or simply is the most readily cultivated.

Keywords

EDTA Electrophoresis Fractionation Bacillus Polyacrylamide 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • T. M. Schmidt
    • 1
  • E. F. Delong
    • 2
  • N. R. Pace
    • 1
  1. 1.Department of Biology and Institute for Molecular and Cellular BiologyIndiana UniversityBloomingtonUSA
  2. 2.Biology DepartmentWoods Hole Oceanographic InstitutionWoods HoleUSA

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