Hydrophobic Grid Membrane Filter Methods for Detection of Foodborne Pathogens
Why the Hydrophobic Grid Membrane Filter (HGMF)?
The HGMF is a square membrane filter (60 x 60 mm, pore size 0.45 μm) printed with a black hydrophobic grid outlining 1600 (40 x 40) small squares. It was developed as a vehicle for electronic counting, and for this purpose it combines desirable features from plate and most probable number (MPN) counts. Some of its unusual properties result from the confining of colony growths to the grid cell in which they originated; a typical appearance after incubation is of a grid carrying randomly distributed square “colonies”.
KeywordsMold Lysine Pseudomonas Lactose Gelatin
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- Anonymous (1989) Methods for the isolation and identification of Salmonella from foods. Method MFHPB-20. In: Compendium of analytical methods: laboratory procedures of microbiological analysis of foods. Minister of Supply and Services Canada, Catalogue No H49–34/3–1989E, Ottawa, CanadaGoogle Scholar
- AOAC (1985) Salmonella detection in foods, hydrophobic grid membrane filter method. J Assoc Off Anal Chem 68:405–407Google Scholar
- AOAC (1986) Salmonella detection in foods, hydrophobic grid membrane filter method. Final Action. J Assoc Off Anal Chem 69:376Google Scholar
- Cerquiera-Campos ML, Peterkin PI, Sharpe AN (1986) Improved immunological membrane filter method for detection of food-borne Salmonella strains. Appl Environ Microbiol 52: 124–127Google Scholar
- Entis P, Boleszczuk P (1983) Overnight enumeration of Vibrio parahaemolyticus in seafood by hydrophobic grid membrane filtration. J Food Prot 46: 783–786Google Scholar
- Farber JM, Peterkin PI, Sharpe AN, D’Aoust J-Y (1985) Enzyme immunoassay-membrane filter method for detection of salmonellae in foods. J Food Prot 48: 790–793Google Scholar
- FDA (1978) Bacteriological analytical manual, 5th edn. Association of Official Analytical Methods, Washington, DCGoogle Scholar
- Peterkin PI, Idziak ES, Sharpe AN (1987) A useful system for propagating and storing genomic libraries. Biotechniques 5: 132–134Google Scholar
- Sharpe AN, Peterkin PI (1988) Membrane filter food microbiology. Innovation in microbiology research studies series. Research Studies Press, Letchworth, UKGoogle Scholar
- Szabo RA, Todd ECD, Jean A (1986) Method to isolate Escherichia toll O157:H7 from food. J Food Prot 49: 768–772Google Scholar