Hydrophobic Grid Membrane Filter Methods for Detection of Foodborne Pathogens

  • A. N. Sharpe


Why the Hydrophobic Grid Membrane Filter (HGMF)?

The HGMF is a square membrane filter (60 x 60 mm, pore size 0.45 μm) printed with a black hydrophobic grid outlining 1600 (40 x 40) small squares. It was developed as a vehicle for electronic counting, and for this purpose it combines desirable features from plate and most probable number (MPN) counts. Some of its unusual properties result from the confining of colony growths to the grid cell in which they originated; a typical appearance after incubation is of a grid carrying randomly distributed square “colonies”.


Grid Cell Much Probable Number Target Organism Yersinia Enterocolitica Bromothymol Blue 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • A. N. Sharpe
    • 1
  1. 1.Bureau of Microbial Hazards, Health Protection BranchHealth and Welfare CanadaTunney’s Pasture, OttawaCanada

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