Nucleotide Sequence Determination As a Diagnostic Tool
Identification of nucleic acids is a valuable tool in diagnostics due to the high specificity of this approach. Nucleic acid hybridization identifies and quantifies specific genes. Hybridization with oligonucleotide probes or nucleotide sequencing can be used to detect genetic variations even as small as point mutations. Widespread diagnostic use of nucleic acid analyses has been limited by the very small amount of analyte present in most applications.
KeywordsAcute Myeloid Leukemia Human Papilloma Virus Affinity Matrix Human Papilloma Virus Type Nucleotide Sequence Variation
Unable to display preview. Download preview PDF.
- Davignon J, Gregg RE, Sing CF (1988) Apolipoprotein E polymorphism and atherosclerosis. Arteriosclerosis 8: 1–21Google Scholar
- Gissman L (1984) Papillomaviruses and their association with cancer in animals and in man. Cancer Sury 3: 161–181Google Scholar
- Mullis KB, Faloona FA (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. In: Wu R, Grossman L, Moldave K (eds) Methods in enzymology, vol 155. Academic Press, New York, pp 335–350Google Scholar