Nucleotide Sequence Determination As a Diagnostic Tool

  • A. C. Syvänen
  • H. Söderlund

Abstract

Identification of nucleic acids is a valuable tool in diagnostics due to the high specificity of this approach. Nucleic acid hybridization identifies and quantifies specific genes. Hybridization with oligonucleotide probes or nucleotide sequencing can be used to detect genetic variations even as small as point mutations. Widespread diagnostic use of nucleic acid analyses has been limited by the very small amount of analyte present in most applications.

Keywords

Leukemia Electrophoresis Polystyrene Biotin Nucleoside 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bos JL, Fearon ER, Hamilton SR, Verlaan-de Vries M, van Boom JH, van der Eb, AJ, Vogelstein B (1987) Prevalence of ras gene mutations in human colorectal cancer. Nature London 327: 293–297PubMedCrossRefGoogle Scholar
  2. Davignon J, Gregg RE, Sing CF (1988) Apolipoprotein E polymorphism and atherosclerosis. Arteriosclerosis 8: 1–21Google Scholar
  3. Farr CJ, Saiki RK, Erlich HA, McCormick F, Marshall CJ (1988) Analysis of RAS gene mutations in acute myeloid leukemia by polymerase chain reaction and oligonucleotide probes. Proc Natl Acad Sci USA 85: 1629–1633PubMedCrossRefGoogle Scholar
  4. Gissman L (1984) Papillomaviruses and their association with cancer in animals and in man. Cancer Sury 3: 161–181Google Scholar
  5. Hultman T, Stahl S, Homes E, Uhlen M (1989) Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucl Acids Res 17: 4937–4946PubMedCrossRefGoogle Scholar
  6. Mullis KB, Faloona FA (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. In: Wu R, Grossman L, Moldave K (eds) Methods in enzymology, vol 155. Academic Press, New York, pp 335–350Google Scholar
  7. Paik Y-K, Chang DJ, Reardon CA, Davies GE, Mahley RW, Taylor JM (1985) Nucleotide sequence and structure of the human apolipoprotein E gene. Proc Natl Acad Sci USA 82: 3445–3449CrossRefGoogle Scholar
  8. Syvänen A-C, Aalto-Setälä K, Kontula K, Söderlund H (1989) Direct sequencing of affinity-captured amplified human DNA: application to the detection of apolipoprotein E polymorphism. FEBS Lett 258: 71–74CrossRefGoogle Scholar
  9. Thompson G (1988) HLA disease associations: models for insulin dependent diabetes mellitus and the study of complex human genetic disorders. Annu Rev Genet 22: 31–50CrossRefGoogle Scholar
  10. Van Brunt J (1990) Amplifying genes: PCR and its alternatives. Bio/Technology 8: 291–294PubMedCrossRefGoogle Scholar
  11. Voss H, Schwager C, Wirkner U, Sproat B, Zimmermann J, Rosenthal A, Erfle H, Stegemann J, Ansorge W (1989) Direct genomic fluorescent on-line sequencing and analysis using in vitro amplification of DNA. Nucl Acids Res 7: 2517–2527CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • A. C. Syvänen
  • H. Söderlund
    • 1
  1. 1.BiotechnologyOrion PharmaceuticaHelsinkiFinland

Personalised recommendations