Correlation Between TCRV Gene Usage and Antigen Specificities in Human γδ T Cells
In peripheral blood of normal donors a major fraction of γδ cells express a disulphide-linked (Cγ1) receptor with Vγ9 and Vδ2 gene products (Casorati 1989, Groh 1989). Since cells bearing Vγ9/Vδ2 TCR constitute only a minor fraction of γδ cells in the postnatal thymus (Casorati 1989) these cells might be selectively expanded in the periphery. The expansion of Vγ9/vδ2 cells may occur after stimulation with antigen (Parker 1990), or it may be dependent on encountering ligands that bind to the Vγ or vδ, irrespective of the specificities encoded by the V-(D)-J junctional sequences. To discriminate between these possibilities we investigated the antigen reactivity of Vγ9/vδ2 bearing cells. Here we describe two frequent reactivities which correlate with the V gene usage and might explain expansion in peripheral blood.
KeywordsAntigen Reactivity Frequent Reactivity Versus Gene Usage Postnatal Thymus Basel Institute
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- Groh, V., S. Porcelli, M. Fabbi, L.L. Lanier, L.J. Picker, T. Anderson, R.A. Warnke, A.K. Bhan, J.L. Strominger, and M.B. Brenner. (1989) Human lymphocytes bearing T cell receptor γδ are phenotypically diverse and evenly distributed thoroughout the lymphoid system. J Exp Med 169:1277.PubMedCrossRefGoogle Scholar