Analysis of Primary T Cell Responses to Intact and Fractionated Microbial Pathogens
Freshly isolated human T lymphocytes were tested for their response to mycobacteria, mycobacterial lysates, 2 dimensional (2D) PAGE separated mycobacterial lysates, leishmania and defined leishmanial antigen preparations. While γδ T cells proliferated vigourusly in the presence of mycobacteria and mycobacteria derived lysates, a significant stimulation from 2 D gel separated lysates was not detected. In addition γδ T cells failed to respond towards leishmania or leishmanial components. In the αβ T cell compartment some donors, presumably according to their state of immunity against mycobacteria, responded to mycobacteria, mycobacterial lysates and 2 D gel separated mycobacterial lysates. Neither freshly isolated γδ T cells nor αβ T cells from naive donors did mount a significant immune response against leishmania.
KeywordsArthritis Filtration Tuberculosis Electrophoresis Glutamine
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