The Transitory Complexes Between Photoexcited Rhodopsin and Transducin. Biochemical and Spectroscopic Studies

Bornancin, F.; Pfister, C.

doi:10.1007/978-3-642-76482-0_8

F. Bornancin⁵^nAff4 &
C. Pfister⁵

Part of the book series: Research Reports in Physics ((RESREPORTS))

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Abstract

In the first step of the visual transduction cascade, photoexcited rhodopsin (R*_ret) binds to a GDP-carrying transducin (T_GDP), allowing the release of the GDP from its site on T. We obtained a R*_ret-T_e complex (ret for retinal present, e for nucleotide site empty), undissociable almost indefinitely in a medium with physiological ionic strength. In this complex, rhodopsin is totally locked in the Meta-II conformation (R*_ret). Incubation in a low ionic strength medium partially dissociates T_e (probably a denatured form of transducin). In the presence of GDP, a R*_ret-T_GDP complex is obtained. T_GDP appears to have a high binding affinity to R*_ret in physiological ionic conditions. We show that the binding of T_e or T_GDP on cytoplasmic loops of rhodopsin induces different retroactions on the retinal site in rhodopsin.

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F. Bornancin
Present address: Institut de Pharmacologie, Route des Lucioles, Sophia Antipolis, F-06560, Valbonne, France

Authors and Affiliations

Laboratoire de Biophysique Moléculaire et Cellulaire, CEN-Grenoble, 85X, F-38041, Grenoble, France
F. Bornancin & C. Pfister

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F. Bornancin
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C. Pfister
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Editors and Affiliations

Department of Ophthalmology and Department of Biochemistry and Molecular Biology, University of Florida, 32610, Gainesville, Florida, USA
Paul A. Hargrave
Institut für Biophysik und Strahlenbiologie, Universität Freiburg, Albertstraße 23, W-7800, Freiburg, Fed. Rep. of Germany
Klaus Peter Hofmann
Institut für Biologische Informationsverarbeitung, KFA Jülich, Postfach 1913, W-5170, Jülich, Fed. Rep. of Germany
U. Benjamin Kaupp

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Bornancin, F., Pfister, C. (1992). The Transitory Complexes Between Photoexcited Rhodopsin and Transducin. Biochemical and Spectroscopic Studies. In: Hargrave, P.A., Hofmann, K.P., Kaupp, U.B. (eds) Signal Transduction in Photoreceptor Cells. Research Reports in Physics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76482-0_8

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DOI: https://doi.org/10.1007/978-3-642-76482-0_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-53765-6
Online ISBN: 978-3-642-76482-0
eBook Packages: Springer Book Archive

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