Advertisement

Acclimatization of Micropropagated Plants

  • T. Kozai
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 17)

Abstract

In the last 10 years, micropropagation has shown spectacular development. However, at present the widespread use of micropropagation is restricted for several reasons, one of these being that a high percentage of micropropagated plants can be lost or damaged in the acclimatization stage, that is, during transfer from a tissue culture vessel to soil. Due to the special environment in vitro, it is difficult to produce plants which can be acclimatized to the outside environment.

Keywords

Globe Artichoke Plantlet Growth Dianthus Caryophyllus Environmental Control System Acclimatization Stage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brainerd KE, Fuchigami LH (1981) Acclimatization of aseptically cultured apple plants to low relative humidity. J Am Soc Hortic Sci 106(4):515-518 Conover CA, Poole RT (1984) Acclimatization of indoor foliage plants. Hortic Rev 6: 120 - 154Google Scholar
  2. Debergh PS, Harbaoui Y, Lemeur R (1981) Mass propagation of globe artichoke (Cynara scolymus): evaluation of different hypotheses to overcome vitrification with special reference to water potential. Physiol Plant 53: 181–187CrossRefGoogle Scholar
  3. Desjardins Y, Gosselin A, Lamarre M (1987) Utilisation de Penrichissement carbone et de l’eclairage d’appoint lors de l’acclimatation ex vitro de fraisiers, de framboisiers et d’asperges. Symp Plant micropropagation in horticultural industries. Arlon, Belgium, pp 176–184Google Scholar
  4. De Proft MP, Maene LJ, Debergh PC (1985) Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro. Physiol Plant 65: 375–379CrossRefGoogle Scholar
  5. Fujiwara K, Kozai T, Watanabe I (1987) Measurements of CO2 concentration in stoppered vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of the plantlets. J Agric Meteorol 43 (l): 21–30CrossRefGoogle Scholar
  6. Grout BWW, Aston MJ (1978) Transplanting of cauliflower plants regenerated from meristem culture. 2. Carbon dioxide fixation and the development of photosynthetic ability. Hortic Res 17: 65–71Google Scholar
  7. Grout BWW, Millam S (1985) Photosynthetic development of micropropagated strawberry plantlets following transplanting. Ann Bot 55: 129–131Google Scholar
  8. Hayashi M, Kozai T (1987) Development of a facility for accelerating the acclimatization of tissue-cultured plantlets and the performance of test cultivations. Symp Plant micropropagation in horticultural industries. Arlon, Belgium, pp 123–134Google Scholar
  9. Hayashi M, Nakayama M, Kozai T (1988) An application of the acclimatization unit for growth of carnation explants, and for rooting and acclimatization of the plantlets. Acta Hortic 230: 189–194Google Scholar
  10. Kimball SL, Beversdorf WD, Bingham ET (1975) Influence of osmotic potential on the growth and development of soybean tissue cultures. Crop Sci 15: 750–752CrossRefGoogle Scholar
  11. Kozai T, Iwanami Y (1988) Effects of CO2 enrichment and sucrose concentration under high photon flux on the tissue cultured plantlet growth of carnation (Dianthus caryophyllus L.) during the preparation stage. J Jpn Soc Hortic Sci 57: 255–264Google Scholar
  12. Kozai T, Fujiwara K, Watanabe I (1986) Relation between the culture medium composition and water potential of liquid culture media. J Agric Meteorol 42: 1–6 (in Japanese with English summary)Google Scholar
  13. Kozai T, Iwanami Y, Fujiwara K (1987) Effects of CO2 enrichment on the plantlet growth during the multiplication stage. Plant Tissue Cult Lett 4 (l): 22–26CrossRefGoogle Scholar
  14. Kozai T, Hayashi M, Hirosawa Y, Kodama T, Watanabe I (1988) Environmental control for acclimatization of in vitro cultured plantlet (1) Development of the acclimatization unit for accelerating the plantlet growth and the test cultivation. J Agric Meteorol 42(4):349–358 (in Japanese with English Summary)Google Scholar
  15. Lakso AN, Reisch BI, Mortensen J, Roberts MH (1986) Carbon dioxide enrichment for stimulation of growth of in vitro–propagated grapevines after transfer from culture. J Am Soc Hortic Sci 111 (4): 634–638Google Scholar
  16. Lee N, Wetzstein HY, Sommer HE (1985) Effects of quantum flux density on photosynthesis and chloroplast ultra structure in tissue-cultured plantlets and seedlings of Liquidambar styraciflua L. towards improved acclimatization and field survival. Plant Physiol 78: 637–641PubMedCrossRefGoogle Scholar
  17. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  18. Paques M, Boxus Ph (1987) “Vitrification”: review of literature. Acta Hortic 230:155–166Google Scholar
  19. Read PE, Fellman CD (1985) Accelerating acclimation of in vitro-propagated woody ornamentals. Acta Hortic 166: 15–20Google Scholar
  20. Sutter EG, Hutzell M (1984) Use of humidity tents and antitranspirants in the acclimatization of tissue-cultured plants to the greenhouse. Sci Hortic 23: 303–312CrossRefGoogle Scholar
  21. Sutter E, Langhans RW (1982) Formation of epicuticular wax and its effect on water loss in cabbage plants regenerated from shoot-tip culture. Can J Bot 60: 2986–2902CrossRefGoogle Scholar
  22. Vanderschaeghe AM, Debergh PC (1987) Technical aspects of the control of the relative humidity in tissue culture containers. Symp Plant micropropagation in horticultural industries. Arlon, Belgium, pp 68–76Google Scholar
  23. Wardel K, Dobbs EB, Short KC (1983) In vitro acclimatization of aseptically cultured plantlets to humidity. J Am Soc Hortic Sci 108 (3): 386–389Google Scholar
  24. Ziv M, Meir G, Halevy AH (1983) Factors influencing the production of hardened glaucous carnation plantlets in vitro. Plant Cell Tissue Organ Cult 2: 55–65CrossRefGoogle Scholar
  25. Ziv M, Schwartz A, Fleminger D (1987) Hardening aspects of micropropagated carnation plants having malfunctioning stomata. Symp Plant micropropagation in horticultural industries. Arlon, Belgium, pp 47–54Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • T. Kozai
    • 1
  1. 1.Department of Horticultural Engineering, Faculty of HorticultureChiba UniversityChibaJapan

Personalised recommendations