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Autotrophic Micropropagation

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High-Tech and Micropropagation I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 17))

Abstract

Use of in vitro micropropagated plantlets is currently expanding worldwide; however, its widespread commercial use is still mainly restricted to horticultural crops, because its production cost is relatively high. In order to expand the use of micropropagation beyond horticulture to include agriculture and forestry, a novel mass-propagation system for reducing the production cost by 80-90% is required. The use of autotrophic or sugar-free micropropagation methods described in this chapter may provide a basis for such a system.

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References

  • Charles-Edwards DA (1981) The mathematics of photosynthesis and productivity. Academic Press, New York, pp 22–29

    Google Scholar 

  • De Proft MP, Maene LJ, Debergh PC (1985) Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro. Physiol Plant 65: 375–379

    Article  Google Scholar 

  • Desjardins Y, Laforge F, Lussier C, Gosselin A (1988) Effect of CO2 enrichment and high photosynthetic photon flux on the development of autotrophy and growth of tissue-cultured strawberry, raspberry and asparagus plants. Acta Hortic 230: 47–57

    Google Scholar 

  • Flores H, Pickard J, Hoy M (1988) Production of polyacetylenes and thiophenes in heterotrophic and photosynthetic root cultures of Asteraceae. In: Bioactive molecules Vol 7. Elsevier, Amsterdam, pp 233–254

    Google Scholar 

  • Fujiwara K, Kozai T, Watanabe I (1987a) Fundamental studies on environments in plant tissue culture vessels (3) Measurements of carbon dioxide gas concentration in stoppered vessels containing tissue-cultured plantlets and estimates of net photosynthetic rates of the plantlets. J Agric Meteorol 43(l):21–30 (in Japanese with English summary)

    Google Scholar 

  • Fujiwara K, Kozai T, Nakajo Y, Watanabe I (1987b) Measurements and control of physical environment in plant tissue culture vessels (3). Abstr Annu Meet Agric Meteorol Jap, pp 196–197 (in Japanese)

    Google Scholar 

  • Fujiwara K, Kozai T, Watanabe I (1988) Development of a photoautotrophic tissue-culture system for plantlets at rooting and acclimatization stages. Acta Hortic 230: 153–158

    Google Scholar 

  • George EF, Sherrington PD (1984) Plant propagation by tissue culture. Exegetics, Hants, England, pp 223–227

    Google Scholar 

  • Goto E, Takakura T (1987) Relationship between light intensity and light energy requirement in the growth of leaf vegetables under artificial light. J Agric Meteorol 43 (3): 229–232

    Article  Google Scholar 

  • Grout BWW (1988) Photosynthesis of regenerated plantlets in vitro, and the stresses of transplanting. Acta Hortic 230: 129–135

    Google Scholar 

  • Grout BWW, Aston MJ (1978) Transplanting of cauliflower plants regenerated from meristem culture. II. Carbon dioxide fixation and the development of photosynthetic ability. Hortic Res 17: 65-71

    Google Scholar 

  • Grout BWW, Price F (1987) The establishment of photosynthetic independence in strawberry cultures prior to transplanting. Proc Symp Plant micropropagation in horticultural industries. Arlon, Belgium, pp 55–60

    Google Scholar 

  • Hayashi M, Kozai T (1987) Development of a facility for accelerating the acclimatization of tissuecultured plantlets and the performance of test cultivations. Proc Micropropagation in Horticultural Industries (Belgium), pp 123–134

    Google Scholar 

  • Hayashi M, Nakayama M, Kozai T (1988) An application of the acclimatization unit for growth of carnation explants, and rooting and acclimatization of the plantlets. Acta Hortic 230: 189–194

    Google Scholar 

  • Hartmann HT, Kester DE (1983) Plant propagation. Prentice-Hall, Englewood Cliffs, pp 316–326

    Google Scholar 

  • Hirosawa Y, Kozai T (1987) Environmental control system for an acclimatization unit. Nogyo Oyobi Engei 62 (6): 776–781

    Google Scholar 

  • Honjo T, Takakura T (1987) Effects of C02 concentration, light intensity and liquid medium composition for the growth of Cymbidium PLB. J Agric Meteorol 43(3):223-227 (in Japanese with English summary) Hon H ( 1966 ) Gravel culture of vegetable and ornamental crops. Agri Hort Yoken-do, Tokyo, pp 210 (in Japanese)

    Google Scholar 

  • Jones HG (1983) Plants and microclimate. Cambridge University Press, Cambridge, pp 130–169

    Google Scholar 

  • Kaneko T, Kato R, Takatsuji M (1988) Effects of carbon source on adventitious root formation of butter-head type lettuce. Abstr Annu Meet Environ Control Biol, Japan, pp 44–45 (in Japanese)

    Google Scholar 

  • Kinoshita T, Kozai T, Fujiwara K (1988) Changes with time in water potential of the culture medium during the multiplication of in vitro plantlets. Abstr Japan Soc Hort Sci Spring Meet, pp 234–235 (in Japanese)

    Google Scholar 

  • Kozai T (1988) High technology in protected cultivation. In: Horticulture in high technology era. A lecture note of a special lecture held in Tokyo, May 1988, pp 1–43

    Google Scholar 

  • Kozai T, Iwanami Y (1988) Effects of CO2 enrichments and sucrose concentration under high photon flux on the tissue cultured plantlets growth of carnation (Dianthiis caryophylliis L.) during the preparation stage. J Jap Soc for Hort Sci 57: 255–264

    Google Scholar 

  • Kozai T, Kubota C (1988) Basic components for promoting autotrophic growth of in vitro plantlets at multiplication and preparation stages. Abstr Japan Soc Hort Sci Spring Meet, pp 402–403 (in Japanese)

    Google Scholar 

  • Kozai T, Sekimoto K (1988) Effects of the number of air changes per hour of the stoppered vessel and the photosynthetic photon flux on the carbon dioxide concentration inside the vessel and the growth of strawberry plantlets in vitro. Environ Control Biol 26(l):21–29 (in Japanese with English summary)

    Google Scholar 

  • Kozai T, Fujiwara K, Watanabe I (1986a) Fundamental studies on environments in plant tissue culture vessels. (1) Relation between the culture medium composition and water potential of liquid culture media. J Agric Meteorol 42(1): 1–6 (in Japanese with English summary)

    Google Scholar 

  • Kozai T, Fujiwara K, Watanabe I (1986b) Fundamental studies on environments in plant tissue culture vessels. (2) Effects of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers. J Agric Meteorol 42(2):119–127 (in Japanese with English summary)

    Google Scholar 

  • Kozai T, Hayashi M, Hirosawa Y, Kodama T, Watanabe I (1987a) Environmental control for acclimatization of in vitro cultured plantlets. (1) Development of the acclimatization unit for accelerating the plantlet growth and the test cultivations. J Agric Meteorol 42(4):349–358 (in Japanese with English summary)

    Google Scholar 

  • Kozai T, Iwanami Y, Fujiwara K (1987b) Effects of CO2 enrichment on the plantlet growth during the multiplication stage. Plant Tissue Cult Lett 4(l):22–26 (in Japanese with English summary)

    Google Scholar 

  • Kozai T, Koyama Y, Watanabe I (1987c) Energy balance of a plant growth lamp with water cooled reflector for reducing cooling load, Abstr Annu Meet Agric Met of Japan, pp 186–187 (in Japanese)

    Google Scholar 

  • Kozai T, Oki H, Fujiwara K (1987d) Environmental control for mass propagation of tissue-cultured plantlets (2). Abstr Japan Soc Hort Sci Spring Meet, pp 366–367 (in Japanese)

    Google Scholar 

  • Kozai T, Oki H, Fujiwara K (1988a) Effects of CO2 enrichment and sucrose concentration under high photosynthetic photon flux on growth of tissue-cultured cymbidium plantlets during the preparation stage. Proc Micropropagation in Horticultural Industries, Arlon, Belgium, pp 135–141

    Google Scholar 

  • Kozai T, Koyama Y, Watanabe I (1988b) Multiplication and rooting of potato plantlets in vitro with sugar-free medium under high photosynthetic photon flux. Acta Hortic 230: 121–127

    Google Scholar 

  • Kozai T, Kubota C, Watanabe I (1988c) Effects of basal medium component on the growth of carnation plantlets in auto- and mixotrophic tissue culture. Acta Hortic 230: 159–166

    Google Scholar 

  • Kozai T, Iwabuchi K, Watanabe I (1988d) Effects of low dark period temperatures and CO2 enrichment during the photoperiod on the growth of strawberry plantlets in vitro (1), (2). Abstr Annu Autumn Meet Jpn Soc Hort Sci: 270-271, 272–273

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Pierik RLM (1987) In vitro culture of higher plants. Martinus Nijhoff, Dordrecht, pp 344

    Book  Google Scholar 

  • Pieters B, Maene L, Buggenhout P (1987) Evolution of some growth factors during tissue culture. Meded Fac Landbouwwet Rijksuniv Gent 52 (4): 1409–1416

    CAS  Google Scholar 

  • Shimada N, Tanaka F, Kozai T (1988) Effects of low 02 concentration on net photosynthesis of C3 plantlets in vitro. Acta Hortic 230: 171–175

    Google Scholar 

  • Thompson MR, Thorpe TA (1987) Metabolic and non-metabolic roles of carbohydrates. In: Bonga JM, Durzan DJ (eds) Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 89–112

    Google Scholar 

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© 1991 Springer-Verlag Berlin Heidelberg

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Kozai, T. (1991). Autotrophic Micropropagation. In: Bajaj, Y.P.S. (eds) High-Tech and Micropropagation I. Biotechnology in Agriculture and Forestry, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76415-8_18

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  • DOI: https://doi.org/10.1007/978-3-642-76415-8_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76417-2

  • Online ISBN: 978-3-642-76415-8

  • eBook Packages: Springer Book Archive

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