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Problems with Explant Exudation in Micropropagation

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High-Tech and Micropropagation I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 17))

Abstract

During the time of organ establishment for the purpose of micropropagation, discoloration of the medium and browning or blackening of the explant is often observed. This may be a sign of potential problems and may mean that special techniques will be required to obtain a healthy-looking and rapidly growing explant. Similar staining of the medium may be observed throughout the in vitro cycle. Although this is a concern for those involved in micropropagation, it has also been noted by researchers working with protoplasts, callus, somatic embryos, and other forms of plant tissue culture.

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  1. Department of Plant and Soil Science, Southern Illinois University, Carbondale, IL, 62901-4415, USA

    M. E. Compton

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  1. J. E. Preece
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  1. A-137, New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

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Preece, J.E., Compton, M.E. (1991). Problems with Explant Exudation in Micropropagation. In: Bajaj, Y.P.S. (eds) High-Tech and Micropropagation I. Biotechnology in Agriculture and Forestry, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76415-8_10

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  • DOI: https://doi.org/10.1007/978-3-642-76415-8_10

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