Abstract
Micropropagation of plants is a multibillion dollar industry being practiced in hundreds of small and large nurseries and biotech labs throughout the world. Presently, it is the only component of plant biotechnology which has been commercially exploited on such a large scale. There are about 1000 plant species that can now be micropropagated (Murashige 1989). Micropropagation has been employed for about half a century, especially for ornamental plants (see Ammirato et al. 1989). Millions of plants are produced, sold locally, or exported to different countries (Chu and Kurtz 1989). Presently, interest has been extended to agricultural/plantation crops (e. g., potato, strawberry, oil palm, banana, etc.), medicinal and aromatic plants (Bajaj et al. 1988), and trees (see Bajaj 1986a, 1989a, 1991), (especially those which are difficult to propagate. Micropropagation of fruit and forest trees offers not only means for the mass multiplication of existing stocks of germplasm for biomass energy production, but also for the conservation of important, elite, and rare plants that are threatened with extinction.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aitken-Christie J, Jones C (1987) Towards automation: Radiata pine shoot hedges in vitro. Plant Cell Tissue Organ Cult 8: 185–196
Akita M, Takayama S (1988) Mass propagation of potato tubers using jar fermentor techniques. Acta Hortic 230: 55–61
Aldrufeu A (1987) Rooting and acclimatization of Pelargonium zonale plantlets. Acta Hortic 212: 361–366
Ammirato PV, Evans DA, Sharp WR, Bajaj YPS (eds) (1989) Handbook of plant cell culture, vol 5. Ornamental species. McGraw-Hill, New York
Anderson WC, Meagher GW (1977) Tissue culture propagation of broccoli, Brassica oleracea, for use in Fi hybrid seed production. Am Soc Hort Sci 102: 69–73
ASAE (ed) (1983) Robotics and intelligent machines in agriculture. Am Soc Agric Eng (ASAE) Pubi 4–84: 155 pp
ASAE (ed) (1985) Agri-Mation I. ASAE Pubi 01–85, 375 pp
Azechi S, Hashimoto T, Nakashizuka M, Murata M, Ishii H (1985) Batch culture of tobacco plant cells in high concentration medium. Jpn Tob Salt Pubi Corp 20: 61–71
Cachita CD, Cristea V, Achim F, Gergely K (1985b) Actiunea acidului 2-cloroetilfosfonic asupra inoculilor cultivati “in vitro”. In: Ill-lea Simp, Nat de Culturi de Celule si Tesuturi Vegetale, Bucuresti, pp 463–474
Cachita CD, Cristea V, Gergely K (1985c) Multiplicarea “in vitro” si micropropagarea pe medii aseptice la Montbretia crocosmiflora Lemoine. In: Ill-lea Simp Nat de Culturi de Celule si Tesuturi Vegetale, Bucuresti, pp 325–330
Cachita CD, Cristea V, Badiu A (1991a) Researches on the in vitro morphogenesis at the level of different kinds of sugar beet explants. In: IV-lea Simp Nat de Culturi de Tesuturi si Celule Vegetale, Cluj-Napoca (in press)
Bajaj YPS (ed) (1989a) Biotechnology in agriculture and forestry, vol 5. Trees II. Springer, Berlin Heidelberg New York
Cachita CD, Petrescu C, Stanila M, Cristea V, Gergely M, Pataky L (1988) Organogenesis in vitro-theoretical and practical aspects in ornamentals. In: 2nd Nordic Symp on Cell and tissue culture. EUCARPIA Int Congr on Genetic manipulation in plant breeding, Helsing0r (Elsinore), Denmark (Abstr), 16 pp
Bajaj YPS (1990a) Cryopreservation of germplasm of vegetatively propagated crops. Bull Soc bot Fr 137: 99–114
Bajaj YPS (1990b) Cryopreservation of germplasm of wheat. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 13. Wheat. Springer, Berlin Heidelberg New York, pp 669–681
Cachita CD, Zapirtan M, Grigoras S (1991d) In vitro cultured Drosera rotundifolia - a new biotest. In: IV-lea Simp Nat de Culturi de Tesuturi si Celule Vegetale, Cluj-Napoca (in press)
Bajaj YPS (ed) (1991) Biotechnology in agriculture and forestry, vol 16. Trees III. Springer, Berlin Heidelberg New York
Bajaj YPS, Sala F (1991) Cryopreservation of germplasm of rice (Oryza sativa L.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 14. Rice, Springer, Berlin Heidelberg New York, pp 553–571
Chlyah H (1984) Correlations in organogenesis. In: Novak FJ, Havel L, Dolezel J (eds) Plant tissues and cell culture application to crop improvement. Czechoslovak Acad Sci, Prague, pp 57–66
Boxus Ph (1985) Big losses due to bacterial contamination can be avoided in mass propagation scheme. Symp In vitro problems related to mass propagation of horticultural crops, Gembloux
Brainerd KE, Fuchigami LH (1981) Acclimatization of aseptically cultured apple plants to low relative humidity. J Am Soc Hort Sci 106: 515–518
Brochard P (1987) Use of MILAP substrates for in vitro rooting and coming out of plants obtained by micropropagation. Acta Hortic 212: 322–323
Lazàr M, Cachità CD (1985a) Immultirea “in vitro” a plantei Exacum afine Balf. In: Ill-lea Simp Nat de Culturi Celule si Tesuturi Vegetale, Bucuresti, pp 321–324
Challa H, Bot GPA, Nederhoff EM, Van de Braak NJ (1988) Greenhouse climate control in the nineties. Acta Hortic 230: 459–470
Chee RP, Cantliffe DJ (1988) Propagule sorting by computerized vision. HortSci 23: 173
Murashige T (1984) Parameters in regenerating plants “in vitro”. In: Novak FJ, Havel L, Dolezel J (eds) Plant tissue and cell culture application to crop improvement. Czechoslovak Acad Sci, Prague, pp 23–31
Compton ME, Preece JE (1988) Effects of phenolic compounds on tobacco callus and blackberry shoot cultures. J Am Soc Hort Sci 113: 160–163
Conover CA, Poole RT (1987) Acclimatization of indoor foliage plants. Hort Rev 6: 120–154
Daguin F, Letouzé R (1986) Ammonium-induced vitrification in cultured tissues. Physiol Plant 66: 94–98
Debergh PC (1987) Improving micropropagation. IAPTC Newslett 51: 1–10
Debergh PC, Maene LJ (1981) A scheme for commercial propagation of ornamental plants by tissue culture. Sci Hortic 14:335–345
De Bry L (1986) Robots in plant tissue culture: an insight. IAPTC Newslett 49: 2–22
Deleplanque H, Bonnet P, Postaire JPG (1985) An intelligent robotic system for in vitro plantlet production. Révisée (IFS Publ) 5: 305–314
Desjardins Y, Laforge F, Lussier C, Gosselin A (1988) Effect of C02 enrichment and high photosynthetic photon flux on the development of autotrophy and growth of tissue-cultured strawberry, raspberry and asparagus plants. Acta Hortic 230: 45–53
Donnan A (1986) Determining and minimizing production costs. In: Zimmerman RJ, Griesbach FA, Hammerschlag F, Lawson RH (eds) Tissue culture as a plant production system for horticultural crops. Martinus Nijhoff, Dordrecht, pp 167–173
Druart Ph, Pâques M, Boxus Ph (1984) Morphologie anormale semblable aux symptômes de vitrification reccontres au cours de la micropropagation de Prunus glaudulosa Thurn. var. sinensis. Influence de l’AVG, de l’AIBA et d’AgN03. Arch Int Physiol Biochem 92: V22–V23
Einset JW (1985) Role of cytokinins in woody plant micropropagation. Proc Int Plant Prop Soc 35: 608–615
Fowler MW (1987) Process systems and approaches for large scale plant cell culture. In: Green CE et al. (eds) Plant tissue and cell culture. Alan R Liss, New York, pp 459–471
Fujita N (1989) Application of robotics to micropropagation system. In Vitro Cell Dev Biol 25 (3): 22A
Fujita Y (1988) Shikonin: production by plant (Lithospermum erythrorhizon) cell cultures. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 225–236
Gaspar Th, Kevers C, Debergh P, Maene L, Paques M, Boxus Ph (1987) Vitrification: morphological, physiological, and ecological aspects. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry, vol 1. Martinus Nijhoff, Dordrecht, pp 152–166
Giles KL, Morgan WM (1987) Industrial scale plant micropropagation. TIBTECH 5: 35–39
Grand d’Esnon, Harrell R, Chee R (1987) Propagule sorting by computerised vision during artificial seed production. Proc Moet–Hennessy Conf Electronics management of living plants, Monte Carlo, p 22
Harazono Y, Taenaka T, Yabuki K (1984) Optimizing environmental control of greenhouse climate for cucumber growth by means of “learning control method”. Acta Hortic 148: 259–265
Harazono Y, Kamiya H, Yabuki K (1988) A control method based on an artificial intelligence technique and its application for controlling plant environment. Acta Hortic 230: 209–214
Harper PC (1989) Myth and money in micropropagation. Proc Int Plant Prop Soc 38: 232–246
Harrell R, Simonton W (1986) Automation opportunities in plant tissue culture operations. Am Soc Agric Eng Pap 86: p 1596
Hashimoto T, Azechi S (1988) Bioreactors for the large-scale culture of plant cells. In: Bajaj YPS (ed)
Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plant I. Springer, Berlin Heidelberg New York, pp 104–122
Hashimoto Y, Fukuyama T (1985) Some speaking plant approaches to the synthesis of control system in the greenhouse. Acta Hortic 174: 219–226
Hayashi T, Nakayama M, Kozai T (1988) An application of the acclimatization unit for growth of carnation explants, and for rooting and acclimatization of plantlets. Acta Hortic 230: 189–194
Hempel M (1989) Microcomputers in micropropagation. Acta Hortic 226: 699–702
Hussey G (1980) In vitro propagation. In: Ingram DS, Helgeson JP (eds) Tissue culture methods for plant pathologists. Blackwell, Oxford, pp 51–61
Japan Greenhouse Horticulture Association (1988) Areas of glasshouses, plastic houses etc. for horticulture
Jones JB (1989) Economic considerations in tissue culture propagation of ornamentals. In: Ammirato PV, Evans PV, Sharp WR, Bajaj YPS (eds) Handbook of plant cell culture, vol 5. Ornamental species. McGraw-Hill, New York, pp 165–178
Kevers C, Coumans M, Coumans-Gilles MF, Gaspar Th (1984) Physiological and biochemical events leading to vitrification in plants cultured in vitro. Physiol Plant 61: 69–74
Kozai T, Itou T, Inayama M, Kobayashi H (1982) Development of microcomputer-based environment control and data-logging system for greenhouses and its field test. J Agric Meteorol 38: 45–50
Kozai T, Iwanami Y, Fujiwara K (1987) Effects of CO2 enrichment on the plantlet growth during the multiplication stage. Plant Tissue Cult Lett 4 (l): 22–26
Kurata K (1988) Greenhouse control by machine learning. Acta Hortic 230: 195–200
Kutz LJ, Miles GE, Hammer PA, Krutz GW (1987) Robotic transplanting of bedding plants. Trans ASAE 30 (3): 586–590
Lesham B, Shaley DP, Izhar S (1988) Cytokinin as an inducer of vitrification in melon. Ann Bot 61: 255–260
Levin R, Vasil IK (1989) Progress in reducing the cost of micropropagation. IAPTC Newslett 59: 2–12
Levin R, Gaba V, Tal B, Hirsch S, DeNola S, Vasil IK (1988) Automated plant tissue culture for mass propagation. Bio/Tech 6: 1035–1040
Long RD (1987) Economic and strategic aspects of micropropagation. Proc Ireland Bot Meet 1987, pp 95–102
Maene L, Debergh P (1985) Liquid medium additions to established cultures to improve elongation and rooting in vitro. Plant Cell Tissue Organ Cult 5: 127–133
Margaritis A, Wallace JB (1984) Novel bioreactor systems and their applications. Bio/Tech 2:447–453
Maurice V, Vandercook CE, Tisserat B (1985) Automated plant surface sterilization system: Physiol Veg 23: 127–133
McCown DD (1985) Plug systems for micropropagules. In: Zimmerman RH et al. (ed) Tissue culture as a plant production system for horticultural crops. Martinus Nijhoff, Dordrecht, pp 53–60
Meyer MJ, McClelland MT, Smith MAL, Spomer LA (1988) Image analysis of growth and development parameters in microcultured whole plants. HortSci 23 (3): 173
Miwa Y (1989) Robot in tissue culture nursery. In: Measurement, control and information in production of plants. Keisoku Jidou Seigyo Gakkai, Tokyo, pp 202–210 (in Japanese)
Moncousin C (1987) Recent developments in the field of adventitious rhizogenesis of in vitro cuttings. In: Plant micropropagation in horticultural industries. Florizel 87: 79–91
Murashige T (1989) Plant propagation by tissue culture: practice with unrealized potential. In: Ammirato PV, Evans DA, Sharp WR, Bajaj YPS (eds) Handbook of plant cell culture, vol 5. Ornamental species. McGraw-Hill, New York, pp 3–9
N Smeth G (1986) Induction of rooting. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 1. Trees I. Springer, Berlin Heidelberg New York, pp 49–64
Pierik RLM (1988) Handicaps for the large scale commercial application of micropropagation. Acta Hortic 230: 63–71
Pierik RLM (1990) Rejuvenation and micropropagation. IAPTC Newslett 62: 11–21
Preil W, Florek P, Wix U, Beck A (1988) Towards mass propagation by use of bioreactors. Acta Hortic Rajbhandary SB, Bajaj YPS (1991) Rooting of in vitro produced shoots in non-sterile sand — an inexpensive and efficient technique for en masse micropropagation. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 17. High-tech and micropropagation I. Springer, Berlin Heidelberg New York pp 262–269
Read PE, Fellman CD (1985) Accelerating acclimation of in vitro propagated woody ornamentals. Acta Hortic 166: 15–20
Redenbaugh K, Slade D, Viss P, Fujii J (1987) Encapsulation of somatic embryos in synthetic seed coats. HortSci 22: 803
Reinert J, Bajaj YPS, Zbell B (1977) Aspects of organization - organogenesis, embryogenesis, cytodifferentiation. In: Street HE (ed) Plant tissue and cell culture. Blackwell, Oxford, pp 389–427
Rittershaus E, Ulrich J, Westphal K (1990) Large-scale production of plant-cell cultures. IAPTC Newslett 61: 2–10
Rowe JW (1986) New technologies in plant tissue culture. In: Zimmerman RH et al. (ed) Tissue culture as a plant production system for horticultural crops. Martinus Nijhoff, Dordrecht, pp 35–51
Schiel O, Berlin J (1987) Large-scale fermentation and alkaloid production of cell suspension cultures of Cathranthus roseus. Plant Cell Tissue Organ Cult 8: 153–161
Seginer I (1989) Optimal greenhouse production under economic constraints. Agric Systems 29: 67–80
Sluis CJ, Walker KA (1985) Commercialization of plant tissue culture. IAPTC Newslett 47: 2–12
Smart NJ, Fowler MW (1984) An airlift column bioreactor suitable for large-scale cultivation of plant cell suspensions. J Exp Bot 35: 531–535
Sonneveld C, Welles GWH (1984) Growing vegetables in substrates in the Netherlands. Proc Vlth Int Congr Soilless Culture, pp 613–632
Sonneveld C, Welles GWH (1988) Yield and quality of rock wool-grown tomatoes as affected by variations in EC-value and climatic conditions. Plant Soil 111:37–42
Stafford JEH (1985) Experience with a robotic analytical system. Lab Pract 34: 19–22
Stuart DA, Strickland SG, Walker KA (1987) Bioreactor production of alfalfa somatic embryos. HortSci 22: 800–803
Styler DJ (1985) Bioreactor technology for plant propagation. Basic Life Sci 32: 117–130
Sutter E, Hutzell M (1984) Use of humidity tents and antitranspirants in the acclimatization of tissuecultured plants to the greenhouse. Sci Hortic 23: 303–312
Takayama S (1984) Mass propagation of plants through shake culture techniques. Plant Tissue Cult Lett 1: 8–13
Tanaka H, Nishijima F, Suwa M, Iwamoto T (1983) Rotating drum fermenter for plant cell suspension cultures. Biotech Bioeng 25: 2359–2370
Tanaka M, Goi M, Higashiura T (1988a) A novel disposable culture vessel made of fluorocarbon polymer films for micropropagation. Acta Hortic 226: 663–670
Tanaka M, Jinno K, Goi M, Higashiura T (1988b) The use of disposable fluorocarbon polymer film culture vessel in micropropagation. Acta Hortic 230: 73–80
Ting KC, Giacomelli CE, Roberts WJ (1988) SCAR A robots work cell for seedling transplantation. Acta Hortic 230: 221–228
Tisserat B, Vandercook CE (1985) Development of an automated plant culture system. Plant Cell Tissue Organ Cult 5: 107–117
Tisserat B, Vandercook CE (1986) Computerized long-term tissue culture for orchids. Am Orchid Soc Bull 55: 35–42
Ulrich B, Wiesner W, Arens H (1985) Large-scale production of rosmarinic acid from plant cell cultures of Coleus blumei Benth. In: Neumann KH, Barz W, Reinhard E (eds) Primary and secondary metabolism of plant cell cultures. Springer, Berlin Heidelberg New York, pp 293–303
Ushiyama K, Miyamoto Y, Oda H, Ishida Y (1984) Gaseous phase culture equipment for continuous culture of plant tissues. Jpn Patent Kokai Tokyo Koho 59–45873 (in Japanese)
Ushiyama K, Oda H, Miyamoto Y (1986) Large scale tissue culture of Panax ginseng roots. Abstr VI Int Congr Plant tissue and cell culture. Minnesota Univ, 252 pp
Vanderschaege A, Debergh P (1987) Automation of tissue culture manipulations in the final stages. IAPTCNewslett 51: 10
Wagner F, Vogelmann H (1977) Cultivation of plant tissue culture in bioreactors and formation of secondary metabolites. In: Barz W, Reinhard E, Zenk MH (eds) Plant tissue culture and its biotechnological application. Springer, Berlin Heidelberg New York, pp 245–252
Wardel K, Dobbs EB, Short KC (1983) In vitro acclimation of aseptically cultured plantlets to humidity. J Am Soc Hort Sci 108: 386–389
Weathers PJ, Giles KL (1988) Regeneration of plants using nutrient mist culture. In Vitro 24 (7): 727–732
Withers LA, Bajaj YPS (1982) The establishment of plant tissue cultures and the regeneration of plants from cultures. In: Withers LA, Williams JT (ed) Crop genetic resources–the conservation of difficult material. IUBS, Ser B 42, FAO Rome, pp 93–96
Zimmerman RH, Yae BW, Fordham I (1987) Comparison of rooting methods for apple cultivars in vitro. Acta Hortic 212: 303–310
Zyrd J-P (1987) Image analysis: its uses in the analysis of plant cells and protoplasts. Symp Innovations and plant production. Monte Carlo, Monaco, Sept 1987, p 21
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Bajaj, Y.P.S. (1991). Automated Micropropagation for en masse Production of Plants. In: Bajaj, Y.P.S. (eds) High-Tech and Micropropagation I. Biotechnology in Agriculture and Forestry, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76415-8_1
Download citation
DOI: https://doi.org/10.1007/978-3-642-76415-8_1
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-76417-2
Online ISBN: 978-3-642-76415-8
eBook Packages: Springer Book Archive