Abstract
In order to discuss the quality of a HPLC column the plate number N must be known. It is usually calculated from the ratio of retention time tR and total peak width at half height w0.5 (1). However the plate number should only be calculated from the chromatographic peak broadening. Real measured w0.5 consists of additional start width from the volume of the sample loop and additional broadening from diffusion in the mobile phase. Therefore N calculated from different peaks is different, though it should be a feature of the column. By linear regression chromatographic peak broadening can be distinguished from other ones. Thereby the calculation of plate numbers is improved, simultaneously a measure for precision of this estimation is obtained. The importance of automatisation and spline-algorithms for a proper determination of the peak maximum and the peak width at half height is demonstrated.
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© 1991 Springer-Verlag Berlin Heidelberg
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Ebel, S., Wätzig, H. (1991). Validation by Automatisation: An Improved Method to Test Columns. In: Gmehling, J. (eds) Software Development in Chemistry 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76325-0_17
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DOI: https://doi.org/10.1007/978-3-642-76325-0_17
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-53532-4
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