Mutation: Construction and Analysis of Cyanobacterial Mutants in Photosynthesis Research

  • Christer Jansson
  • Anders Lönneborg
Part of the Progress in Botany/Fortschritte der Botanik book series (BOTANY, volume 52)

Abstract

Mutants in cyanobacteria, like in many other organisms, have been used to elucidate metabolic pathways, composition of macromolecular structures, uptake mechanisms for nutrients, and other phenomena in the cell. The importance of cyanobacterial mutagenesis has been particularly evident in photosynthesis research. The reasons for this are twofold: (1) Cyanobacteria are prokaryotes and amenable to a variety of genetic manipulations that are difficult to perform with plants; (2) photosynthesis in chloroplasts and cyanobacteria are basically similar. Thus, a fruitful approach to learn about plant photosynthesis is to study the simpler and genetically more favorable cyanobacterial system. The purpose of this review is to make the reader familiar with the construction and analysis of cyanobacterial mutants and their use in photosynthesis research. For methodological aspects we refer to reviews by Golden (1988), Joset (1988), Porter (1988) and Williams (1988).

Keywords

Steam Recombination Polypeptide Photosynthesis Methionine 

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References

  1. Ajlani G, Meyer I, Vernotte C, Astier C (1989) FEBS Lett 246: 207–210.PubMedCrossRefGoogle Scholar
  2. Astier C, Joset-Espardellier F, Meyer I (1979) Arch Microbiol 120: 93–96.CrossRefGoogle Scholar
  3. Beguin S, Guglielmi G, Rippka R, Cohen-Bazire G (1985) Biochimie 67: 109–117.PubMedCrossRefGoogle Scholar
  4. Brusslan J, Haselkom R (1989) EMBO J 8: 1237–1245.PubMedGoogle Scholar
  5. Bryant DA (1988) Genetic analysis of phycobilisome biosynthesis, assembly, structure and function in the Cyanobacterium Synechoccus sp PCC 7002. In: Stevens SE, Bryant DA (eds) Light-energy transduction in photosynthesis: higher plant and bacterial models. The American Society of Plant Physiologists, pp 62–90.Google Scholar
  6. Bryant DA, Rhiel E, De Lorimer R, Zhou J, Stirewalt VL, Gasparich GE, Dubbs JM, Snyder W (1989) Analysis of phyco¬bilisome and photosystem I complexes of Cyanobacteria. In: Baltscheffsky M (ed) Proc 8th Int Congr Photosynthesis Vol II. Kluwer, Dordrecht, pp 1–9.Google Scholar
  7. Chapman JS, Meeks JC (1987) J Gen Microbiol 133: 111–118.Google Scholar
  8. Chauvat F, Rouet P, Bottin H, Boussac A (1989) Mol Gen Genet 216: 51–59.PubMedCrossRefGoogle Scholar
  9. Debus RJ, Barry BA, Babcock GT, Mcintosh L (1988a) Proc Nad Acad Sci USA 85: 427–430.CrossRefGoogle Scholar
  10. Debus RJ, Barry BA, Sithole I, Babcock GT, Mcintosh L (1988b) Biochemistry 27: 9071–9074.PubMedCrossRefGoogle Scholar
  11. Delaney SF, Carr NG (1975) J Gen Microbiol 88:259–268.Google Scholar
  12. Dzelzkalns VA, Bogorad L (1986) J Bacteriol 165:964–971.Google Scholar
  13. Eisenberg Y, Ohad N, Horovitz A, Hirschberg J (1989) Additivity in the contribution to herbicide binding of amino acid residues in die D1 protein of photosystem II. In: Baltscheffsky M (ed) Proc 8th Int Congr Photosynthesis, Vol EI. Kluwer, Dordrecht, pp 641–644.Google Scholar
  14. Golden SS (1988) Methods Enzymol 167:714–727.Google Scholar
  15. Golden SS, Haselkom R (1985) Science 229:1104–1107.Google Scholar
  16. Golden SS, Steams GW (1988) Gene 67:85–96.Google Scholar
  17. Golden SS, Brusslan J, Haselkom R (1986) EMBO J 5:2789–2798.Google Scholar
  18. Golden SS, Dan-Sung CC, Nalty MS (1989) J Bacteriol 171:4707–4713.Google Scholar
  19. Guikema J, Sherman LA (1980) J Bioenerg Biomembr 12:277–295.Google Scholar
  20. Hirschberg J, Ohad N, Pecker I, Rahat A (1987) Z Naturforsch 42c:758–761.Google Scholar
  21. Horowitz A, Ohad N, Hirschberg J (1989) FEBS Lett 243:161–164.Google Scholar
  22. Jansson C, Debus RJ, Osiewacz HD, Gurevitz M, Mcintosh L (1987a) Plant Physiol 85:1021–1025.Google Scholar
  23. Jansson C, Debus RJ, Osiewacz HD, Gurevitz M, Mcintosh L (1987b) Genetic modification of psbA genes in the Cyanobacterium Synechocystis 6803. In: Key JL, Mcintosh L (eds) Plant gene systems and their biology. Alan R Liss, New York, pp 371–381.Google Scholar
  24. Joset F (1988) Methods Enzymol 167:728–735.Google Scholar
  25. Kalla R, Lind LK, Gustafsson P (1989) Mol Microbiol 3:339–347.Google Scholar
  26. Kerby NW, Steward WDP (1988) The biotechnology of microalgae and Cyanobacteria. In: Rogers LJ, Gallon JR (eds) Biochemistry of the algae and cyanobacteria. Oxford Sci Publ, Oxford, pp 319–334.Google Scholar
  27. Klausner A (1986) Bio/Technology 4:947–953.Google Scholar
  28. Kuhlemeier CJ, Logtenberg T, Stoorvogel W, van Heugten HAA, Borrias WE, van Arkel GA (1984) J Bacteriol 159:36–41.Google Scholar
  29. Labarre J, Chauvat F, Thuriaux P (1990) J Bacteriol (in press).Google Scholar
  30. Metz JG, Nixon PJ, R5gner M, Brudvig GW, Diner BA (1989) Biochemistry 28:6960–6969.Google Scholar
  31. Mohamed A, Jansson C (1989) Plant Mol Biol 13:693–700.Google Scholar
  32. Murphy RC, Gasparich GE, Bryant DA, Porter RD (1990) J Bacteriol 172:967–976.Google Scholar
  33. Nilsson F, Andersson B, Jansson C (1989) Structural and ultrastructural organization of thylakoids in a constructed photosystem II mutant of the Cyanobacterium Synechocystis 6803. In: Baltscheffsky M (ed) Proc 8th Int Congr Photosynthesis, Vol I. Kluwer, Dordrecht, pp 299–302.Google Scholar
  34. Nilsson F, Andersson B, Jansson C (1990) Plant Mol Biol 14:1051–1054.Google Scholar
  35. Nixon PJ, Metz JG, Rogner M, Diner BA (1989) A Synechocystis PCC 6803 psbA deletion mutant and its transformation with a psbA gene from a higher plant In: Baltscheffsky M (ed) Proc 8th Int Congr Photosynthesis, Vol I. Kluwer, Dordrecht, pp 471–474.Google Scholar
  36. Ohad N, Hirschberg J (1990) Photosynth Res 23:73–79.Google Scholar
  37. Ohad N, Pecker I, Hirschberg J (1987) Biochemical and molecular analyses of herbicide resistant mutants in Cyanobacteria. In: Biggins J (ed) Progress in Photosynthesis Research, Vol HI. Martinus Nijhoff, Dordrecht, pp 807–810.Google Scholar
  38. Pakrasi H, Williams JGK, Arntzen CJ (1988) EMBO J 7:325–332.Google Scholar
  39. Philbrick JB, Zilinskas BA (1988) Mol Gen Genet 212:418–425.Google Scholar
  40. Pierce J, Carlson TJ, Williams JGK (1989) Proc Nad Sci USA 86:5753–5757.Google Scholar
  41. Porter RD, (1988) Methods Enzymol 167:703–712.Google Scholar
  42. Prentki P, Krisch HM (1984) Gene 29:303–313.Google Scholar
  43. Reiss T, Jansson C, Mcintosh L (1989) Insertion of a chloroplast psbA gene into the chromosome of the Cyanobacterium Synechocystis 6803. In: Baltscheffsky M (ed) Proc 8th Int Congr Photosynthesis, Vol HI. Kluwer, Dordrecht, pp 649–652.Google Scholar
  44. Schaeffer MR, Golden SS (1989a) J Biol Chem 264:7412–7417.Google Scholar
  45. Schaeffer MR, Golden SS (1989b) J Bact 171:3973–3981.Google Scholar
  46. Schaeffer MR, Golden SS (1989b) J Bact 171:3973–3981.Google Scholar
  47. Schaeffer MR, Golden SS (1989b) J Bact 171:3973–3981.Google Scholar
  48. Schaeffer MR, Golden SS (1989b) J Bact 171:3973–3981.Google Scholar
  49. Schaeffer MR, Golden SS (1989b) J Bact 171:3973–3981.Google Scholar
  50. Schaeffer MR, Golden SS (1989b) J Bact 171:3973–3981.Google Scholar
  51. Schaeffer MR, Golden SS (1989b) J Bact 171:3973–3981.Google Scholar
  52. Schaeffer MR, Golden SS (1989b) J Bact 171:3973–3981.Google Scholar
  53. Schaeffer MR, Golden SS (1989b) J Bact 171:3973–3981.Google Scholar
  54. Tandeau de Marsac N, Borrias WE, Kuhlemeier CJ, Castets AM, van Arkel GA, van den Hondel CAMJJ (1982) Gene 20: 111–119.PubMedCrossRefGoogle Scholar
  55. Vermaas WFJ, Williams JGK, Rutherford AW, Mathis P, Arntzen CJ (1986) Proc Natl Acad Sci USA 83: 9474–9477.PubMedCrossRefGoogle Scholar
  56. Vermaas WFJ, Rutherford AW, Hansson O (1988) Proc Natl Acad Sci USA 85: 8477–8481.PubMedCrossRefGoogle Scholar
  57. Williams JGK (1988) Methods Enzymol 167: 766–778.CrossRefGoogle Scholar
  58. Yu J, Vermaas WFJ (1990) Plant Cell 2: 315–322.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Christer Jansson
    • 1
  • Anders Lönneborg
    • 2
  1. 1.Department of Biochemistry, The Arrhenius LaboratoriesStockholm UniversityStockholmSweden
  2. 2.Plant Molecular Biology Laboratory-NLVFNorway

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