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Bioorganic Chemistry Frontiers pp 1–31Cite as

Hybrid Enzymes and the Sequence-Specific Cleavage of Nucleic Acids

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Part of the book series: Bioorganic Chemistry Frontiers ((BIOORGANIC,volume 2))

Abstract

Catalysts have been generated that sequence-specifically hydrolyze RNA and DNA at predefined sites, i.e., a new class of restriction enzymes. New binding domains (oligonucleotides or peptides of defined sequence) were introduced site-selectively into the relatively nonselective phosphodiesterases, staphylococcal nuclease and ribonuclease S, to generate hybrid enzymes that site-specifically cleave nucleic acids. Subsequent mutagenesis of the hybrid enzymes has generated enzymes that siteselectively hydrolyze one bond in large RNAs and DNAs in a catalytic fashion. We have been able to use these hybrid enzymes to selectively cleave duplex plasmid DNAs via D-loop formation. This work has not only resulted in powerful tools for studying RNA and DNA structure but also may provide a general strategy for designing other selective hybrid enzymes for important biological transformations, such as the selective cleavage of genomic DNA, or selective cleavage of peptide amide bonds.

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Author information

Authors and Affiliations

  1. University of California, Berkeley, Berkeley, CA, 94720, USA

    David R. Corey, Ronald N. Zuckermann & Peter G. Schultz

Authors
  1. David R. Corey
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  2. Ronald N. Zuckermann
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  3. Peter G. Schultz
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Editor information

Editors and Affiliations

  1. Département de Chimie, Université de Montréal, Montréal, Quebec, H3C 3J7, Canada

    H. Dugas

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© 1991 Springer-Verlag Berlin Heidelberg

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Corey, D.R., Zuckermann, R.N., Schultz, P.G. (1991). Hybrid Enzymes and the Sequence-Specific Cleavage of Nucleic Acids. In: Dugas, H. (eds) Bioorganic Chemistry Frontiers. Bioorganic Chemistry Frontiers, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76241-3_1

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  • DOI: https://doi.org/10.1007/978-3-642-76241-3_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76243-7

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