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Diagnostic DNA Cytometry of the Urothelium

  • Chapter
Urinary Cytology

Abstract

The subjective cytologic examination of urinary sediment by no means offers an ideal solution to the diagnosis of bladder carcinoma:

  • The sensitivity is only 70%, depending on the grade of tumor malignancy.

  • The specificity is only 80%–95% (Murphy et al. 1986; Rübben et al. 1989).

  • The reproducibility of malignancy grading, at approximately 60%–70%, is poor (Ooms et al. 1989).

  • The prognostic relevance of the cytologic tumor grade is not adequate to have a definite impact on treatment planning (Böcking et al. 1990).

  • The prospective biologic behavior of dysplastic cells cannot be accurately predicted.

  • The labor cost is high for the screening of large sample volumes in subjects at risk.

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References

  • Aikens B, Liedtke C-E (1982) Untersuchungen zur Meßbarkeit und Objektivierbarkeit der Urinzytologie durch ein hochauflösendes mikroskopzytophotometrisches Meßsystem. Urologe [A] 21: 98–101

    Google Scholar 

  • Atkin NB (1971) Modal DNA value and chromosome number in ovarian neoplasias. A clinical and histopathologic assessment. Cancer 27: 1964–1974

    Google Scholar 

  • Auer GU, Caspersson TO, Wallgren AI (1980) DNA content and survival in mammary carcinoma. Anal Quant Cytol 2: 161–165

    PubMed  CAS  Google Scholar 

  • Auffermann W, Repges R, Böcking A (1985) Rapid diagnostic DNA-cytometry with an automatic microscope and a TV-image analysis system. Anal Quant Cytol 6: 179–188

    Google Scholar 

  • Auffermann W, Urquardt M, Rübben H, Wohltmann D, Böcking A (1986) DNA grading of urothelial carcinoma of the bladder. Anticancer Res 6: 27–32

    PubMed  CAS  Google Scholar 

  • Bachmann P, Hinrichsen K (1979) Principles and methods of quantitative determination of Feulgen stained DNA with the Television Texture Analysis System (TAS). Histochemistry 60: 61–69

    PubMed  CAS  Google Scholar 

  • Badalament R-A, Gag H, Whitemore WF, Herr HW, Fair WR, Oeltgen HF, Melamed MR (1986) Monitoring intravesical bacillus Calmette-Guerin treatment of superficial bladder carcinoma by serial flow cytometry. Cancer 58: 2751

    PubMed  CAS  Google Scholar 

  • Badalament R-A, Hermansen DK, Kimmel M, Gay H, Herr HW, Fair W-R, Whitemore WF, Melamed MR (1987) The sensitivity of bladder wash flow cytometry, bladder wash cytology and voided cytology in the detection of bladder carcinoma. Cancer 60: 1423–1427

    PubMed  CAS  Google Scholar 

  • Badalament R-A, Fair WR, Whitemore WF, Melamed MR (1988) The relative value of cytometry and cytology in the management of bladder cancer: the Memorial Sloan Kettering Cancer Center-experience. Semin Urol 6: 22–30

    PubMed  CAS  Google Scholar 

  • Barlogie B (1981) Abnormal cellular DNA content as a marker of neoplasia in man. Eur J Cancer Clin Oncol 20: 1123–1125

    Google Scholar 

  • Barlogie B, Drewinko B, Schumann J (1980) Cellular DNA content as a marker of neoplasia in man. Am J Med 69: 195–203

    PubMed  CAS  Google Scholar 

  • Bergkvist A, Ljungqvist A, Moberger G (1965) Classification of bladder tumors bases on the cellular pattern. Preliminary report of a clinical pathologic study of 300 cases with a minimum follow up of eight years. Acta ChirScand 130: 371–378

    CAS  Google Scholar 

  • Biesterfeld S, Gerves K, Fischer-Wein G, Böcking A (1992) Euploid polyploidization — a frequent phenomenon in nonneoplastic human tissues. J Clin Pathol (submitted)

    Google Scholar 

  • Böcking A (1990) DNA-Zytometrie und Automation in der klinischen Diagnostik. In: Bonk U (ed) Aktuelle klinische Zytologie. Beitr. Onkol 38: 298–347

    Google Scholar 

  • Böcking A, Auffermann W (1986) Algorithm for DNA cytophotometric diagnosis and grading of malignancy. Letter to the editor. Anal Quant Cytol Histol 8: 363

    PubMed  Google Scholar 

  • Böcking A, Adler CP, Common HH, Hilgarth M, Granzen B, Auffermann W (1984) Algorithm for a DNA-cytophotometric diagnosis and grading of malignancy. Anal Quant Cytol 6: 1–8

    PubMed  Google Scholar 

  • Böcking A, Auffermann W, Jocham D, Contractor H, Wohltmann D (1985) DNA grading of malignancy and tumor regression in prostatic carcinoma under hormone therapy. Appl Pathol 3: 206–214

    PubMed  Google Scholar 

  • Böcking A, Chatelain R, Auffermann W, Krüger GRF, Asmus B, Wohltmann D, Schuster C (1986 a) DNA grading of malignant lymphomas I. Prognostic significance reproducibility and comparison with other classifications. Anticancer Res 6: 1205–1215

    PubMed  Google Scholar 

  • Böcking A, Chatelain R, Löhr GW, Reif M, Rosner R, Becker H (1986 b) DNA grading of malignant lymphomas II. Correlation with clinical parameters. Anticancer Res 6: 1216–1223

    Google Scholar 

  • Böcking A, Sanchez L, Stock B, Müller W (1987) Automated DNA cytophotometry. Lab Practice 36: 73–74

    Google Scholar 

  • Böcking A, Chatelain R, Orthen U, Gien G, Kaikreuth G von, Jocham D, Wohltmann D (1988) DNA grading of prostatic carcinoma: Prognostic validity and reproducibility. Anticancer Res 8: 129–136

    PubMed  Google Scholar 

  • Böcking A, Chatelain R, Biesterfeld S, Noll E, Biesterfeld D, Wohltmann D, Goecke C (1989) DNA grading of breast cancer. Prognostic validity, reproducibility and comparison with other classifications. Anal Quant Cytol Histol 11: 73–80

    PubMed  Google Scholar 

  • Böcking A, Pollmann I, Biesterfeld S (1990) DNA grading of malignancy in urothelial carcinoma of the bladder: Prognostic validity, reproducibility and comparison with morphological parameters. 17th Meeting of the European Federation of Cytology Societies, 30 March-1 April 1989, Flims, Switzerland

    Google Scholar 

  • Böcking A, Chatelain R, Biesterfeld S, Sanchez L, Kropff M, Stock B, Müller W (1992) MIAMED-DNA. Interactive system for rapid diagnostic DNA cytometry. Anal Quant Cytol Histol (submitted for publication)

    Google Scholar 

  • Böhm N, Sprenger E, Schlüter G, Sandritter W (1968) Proportionalitätsfehler bei der Feulgen-Hydrolyse. Histochemie 15: 194–203

    PubMed  Google Scholar 

  • Bretton PR, Herr HW, Kimmel M, Fair WR, Whitemore WF, Melamed MR (1989) Flow cytometry as a predictor of response and progression in patients with superficial bladder cancer treated with bacillus Calmette Guerin. J Urol 141: 1332

    PubMed  CAS  Google Scholar 

  • Brugal G, Chassery J-M (1977) Un nouveau systeme d’analyse densitometrique et morphologique des preparations microscopiques. Histochemistry 52: 251–258

    Google Scholar 

  • Brugal G, Quirion C, Vassilakos P (1986) Detection of bladder cancers using a SAMBA 200 cell image processor. Anal Quant Cytol Histol 8: 187–195

    PubMed  CAS  Google Scholar 

  • Chatelain R, Schunck T, Schindler EM, Schindler AE, Böcking A (1989 a) Diagnosis of prospective malignancy in koilocytic dysplasias of the cervix with DNA cytometry. J Reprod Med 34: 505–510

    PubMed  CAS  Google Scholar 

  • Chatelain R, Willms A, Biesterfeld S, Auffermann W, Böcking A (1989 b) Automated Feulgen staining with a temperature controlled staining machine. Anal Quant Cytol Histol 11: 211–217

    PubMed  CAS  Google Scholar 

  • Chin JL, Huben RP, Nava E, Rustum JM, Greco JM, Pantes JE, Frankfurt OS (1985) Flow cytometry analysis of DNA content in human bladder tumors and irrigation fluids. Cancer 56: 1677–1681

    PubMed  CAS  Google Scholar 

  • Coon GS, Schwartz D, Summers JL, Miller AW, Weinstein RS (1986) Flow cytometric analysis of deparaffined nuclei in urinary bladder carcinoma. Comparison with cytogenetic analysis. Cancer 57: 1594–1601

    PubMed  CAS  Google Scholar 

  • Cox DR (1972) Regression models and life tables. J Roy Statist Soc B 34: 187–200

    Google Scholar 

  • Deeley EM (1955) An integrating microdensitometer for biological cells. J Sci Intrum 32: 263–267

    Google Scholar 

  • Delgado R, Mikum G, Hofstädter F (1984) DNA Feulgencytophotometric analysis of single cells from paraffin-embedded tissue. Pathol Res Pract 179: 92–94

    PubMed  CAS  Google Scholar 

  • Devonec M, Darzynkiewicz Z, Kostyrka-Claps MC, Collste L, Whitemore WF, Melamed MR (1982) Flow cytometry of low stage bladder tumors: Correlation with cytologic and cystoscopic diagnosis. Cancer 49: 109–118

    PubMed  CAS  Google Scholar 

  • Farsund T, Hostmark J (1983) Mapping of cell cycle distribution in normal human urinary bladder epithelium. Scand J Urol Hepathol 17: 51–56

    CAS  Google Scholar 

  • Feulgen R, Rossenbeck H (1924) Mikroskopisch-chemischer Nachweis einer Nukleinsäure vom Typus der Thymonucleinsäure und die darauf beruhende elektive Färbung von Zellkernen in mikroskopischen Präparaten. Z Physiol Chem 135: 203–248

    CAS  Google Scholar 

  • Fossa SD (1975) Feulgen-DNA-values in transitional cell carcinoma of the human urinary bladder. Beitr Pathol 155: 44–55

    PubMed  CAS  Google Scholar 

  • Fossa SD (1977) DNA variations in neighbouring epithelium in patients with bladder carcinoma. Acta Pathol Microbiol Immunol Scand [A] 85: 603–610

    Google Scholar 

  • Fossa SD, Kaalhus O (1976 a) Nuclear size and chromatin concentration in transitional cell carcinoma of the human urinary bladder. Beitr Pathol 157: 109–125

    PubMed  CAS  Google Scholar 

  • Fossa SD, Kaalhus O (1976 b) Computer assisted image analysis of Feulgen stained cell nuclei from transitional cell carcinoma of the human urinary bladder. Acta Pathol Microbiol Immunol Scand [A] 85: 590–602

    Google Scholar 

  • Fossa SD, Kaalhus O (1985) The prognostic relevance of nuclear Feulgen DNA in transitional cell carcinoma of the urinary bladder. A long-term follow up study. Eur Urol 11: 418–421

    PubMed  CAS  Google Scholar 

  • Fossa SD, Kaalhus O, Scott-Knudsen O (1977) The clinical and histopathological significance of Feulgen DNA-values in transitional cell carcinoma of the human urinary bladder. Eur J Cancer Clin Oncol 13: 1155–1162

    CAS  Google Scholar 

  • Gahm T, Aikens B (1990) CAESAR — a computer supported measurement system for the enhancement of diagnostics and quality in cytology. Micron Microsc Acta 1/2: 29–55

    Google Scholar 

  • Graumann W (1953) Zur Standardisierung des Schiffschen Reagens. Z Wiss Mikrosc 61: 225–230

    Google Scholar 

  • Gustafson H, Tribukait B, Esposti PL (1982a) DNA profile on tumour progression in patients with superficial bladder tumours. Urol Res 10: 13–18

    PubMed  CAS  Google Scholar 

  • Gustafson H, Tribukait B, Eposti PL (1982b) The prognostic value of DNA analysis in primary carcinoma in situ of the urinary bladder. Scand J Urol Nephrol 16: 141

    PubMed  CAS  Google Scholar 

  • Hedley DW, Rielander ML, Taylor IW, Rugg CA, Musgrove EA (1983) Method for analysis of cellular DNA-content of paraffin embedded pathological material using flow cytometry. J Histochem Cytochem 31: 1333–1335

    PubMed  CAS  Google Scholar 

  • Hedley DW, Philips J, Rugg CA, Taylor IW (1984) Measurements of cellular DNA content as an adjunct to diagnostic cytology in malignant effusions. Eur J Cancer Clin Oncol 20: 749–752

    PubMed  CAS  Google Scholar 

  • Heim S, Mittelman F (1987) Cancer cytogenetics. Liss, New York, pp 239–240

    Google Scholar 

  • Hofstädter F, Jakse G, Lederer B, Mikuz G, Delgado A (1984) Biological behaviour and DNA cytophotometry of urothelial bladder carcinoma. Br J Urol 56: 289–295

    Google Scholar 

  • Hostmark JG, Laerum OD, Farsund T (1984) DNA aberrations of bladder mucosa in patients with transitional cell carcinomas. Scand J Urol Nephrol 18: 113–120

    PubMed  CAS  Google Scholar 

  • Huber JC (1986) Numerische und strukturelle Chromosomenaberrationen bei gynäkologischen Malignomen. Thieme, Stuttgart

    Google Scholar 

  • Jitsukava S, Tachibana M, Nakazomo M, Tazaki H, Addonizio JC (1987) Flow cytometry based on heterogeneity index score compared with urine cytology to evaluate their diagnostic efficacy in bladder tumor. Urology 29: 218–222

    Google Scholar 

  • Klein F, White FKH (1988) Flow cytometry deoxiribonucleic acid determinations and cytology of bladder washing: practical experience. J Urol 139: 275–278

    PubMed  CAS  Google Scholar 

  • Klein FA, Herr HW, Sogani PC, Whitemore WF, Melamed MR (1982) Detection and follow-up of carcinomas of the urinary bladder by flow cytometry. Cancer 50: 389–395

    PubMed  CAS  Google Scholar 

  • Koss LG, Bartels PH, Bibbo M, Freed SZ, Taylor J, Wied GL (1975) Computer discrimination between benign and malignant urothelial cells. Acta Cytol 19: 378–391

    PubMed  CAS  Google Scholar 

  • Koss LG, Bartels PH, Sychra JJ, Wied GF (1978 a) Computer discriminant analysis of atypical urothelial cells. Acta Cytol 22: 382–386

    PubMed  CAS  Google Scholar 

  • Koss LG, Bartels PH, Sychra JJ, Wied GL (1978b) Diagnostic cytologic sample profiles in patients with bladder cancer using TICAS System. Acta Cytol 22: 392–397

    PubMed  CAS  Google Scholar 

  • Koss LG, Bartels PH, Wied GL (1980) Computer-based diagnostic analysis of cells in the urinary sediment. J Urol 123: 846–849

    PubMed  CAS  Google Scholar 

  • Koss L, Eppide EM, Melder KH, Wersto R (1987) DNA cytophotometry of voided urine sediment. Comparison of cytologic diagnosis and image analysis. Anal Quant Cytol Histol 9: 398–404

    PubMed  CAS  Google Scholar 

  • Lederer B, Mikuz G, Gütter W, Nedden G zur (1972) Cytophotometric investigations of the DNA content of benign and malignant transitional cell tumors of the bladder. Correlation of cytophotometric results with histological grading. Beitr Pathol 147: 379–389

    PubMed  CAS  Google Scholar 

  • Levi P, Cooper EH, Anderson CK, Patu MC, Williams RE (1969) Analysis of DNA content, nuclear size and cell proliferation of transitional cell carcinoma in man. Cancer 23: 1074–1085

    PubMed  CAS  Google Scholar 

  • Ley H, Valet G, Lehmer A, Hartung R (1989) Automatic identification of bladder tumor cells by multiple parameters in flow-cytometry. J Urol 141: 294 A (abstract 497)

    Google Scholar 

  • Marschner S (1992) DNA-Malignitätsgrading des Mammakarzinoms. Prognostische Validität und Reproduzierbarkeit. Dissertation, RWTH Aachen

    Google Scholar 

  • Montironi R, Scarpelli M, Pisani E, Ausuini G, Marinelli F, Marizzi G (1985) Noninvasive papillary transitional-cell tumors. Kayometric and DNA-content analysis. Anal Quant Cytol Histol 7: 337–342

    PubMed  CAS  Google Scholar 

  • Mostofi FK, Sobin LH, Torloni H (1973) Histological typing of urinary bladder tumors. International classification of tumors. WHO, Geneva

    Google Scholar 

  • Murphy WH, Emerson LD, Chandlers RW, Moinuddin SM, Soloway MS (1986) Flow cytometry versus urinary cytology in the evaluation of patients with bladder cancer. J Urol 136: 815–821

    PubMed  CAS  Google Scholar 

  • Ooms EC, Anderson WA, Alons CL, Boon ME, Veldhiuzen RW (1983) Analysis of the performance of pathologists in the grading of bladder tumors. Hum Pathol 14: 144–150

    PubMed  CAS  Google Scholar 

  • Parry W, Hemstreet GP (1988) Cancer detection by quantitative fluorescence image analysis. J Urol 139: 270–274

    PubMed  CAS  Google Scholar 

  • Pauwels RP, Smeets WW, Geraedts JP, Debruyne FM (1987) Cytogenetic analysis in urothelial cell carcinoma. J Urol 137: 210–215

    PubMed  CAS  Google Scholar 

  • Pauwels RPE, Smeets AWGB, Schapers RWM, Geraedts JPM, Debruyne FMJ (1988) Grading in superficial bladder cancer. Cytogenetic classification. Br J Urol 61: 135–139

    PubMed  CAS  Google Scholar 

  • Pfitzer P, Vyska K, Stecher G (1976) Analysis of DNA histograms by computer. Beitr Pathol 159: 157–185

    PubMed  CAS  Google Scholar 

  • Ploem JS (31 8 1989) Das Leitz-MIAC-System; erweiterte Fragestellungen. Arbeitstreffen: Bestandsaufnahme und Perspektiven der Zytoautomation. Institut für Physikalische Elektronik, University of Stuttgart

    Google Scholar 

  • Ploem JS, Verwoerd N, Bonnet J, Koper G (1979) An automated microscope for quantitative cytology combining television image analysis and stage scanning microphotometry. J Histochem Cytochem 27: 136–143

    PubMed  CAS  Google Scholar 

  • Ploem-Zaaijer JJ, Beyer-Boon ME, Leyte-Veldstra L, Ploem JS (1979) Cytofluorometric and cytophotometric DNA measurements of cervical smears stained using a new bicolor method. In: Pressman NJ, Wied GL (eds) Automation of cancer cytology and cell image analysis. Tutorials of Cytology, Chicago, pp 225–235

    Google Scholar 

  • Ratliff JE, Klein FA, White FKH (1985) Flow cytometry of ethanol-fixed versus fresh bladder barbotage specimens. J Urol 133: 958–960

    PubMed  CAS  Google Scholar 

  • Roels F (24 2 1990) Personal communication. CAAC-Meeting, Brussels

    Google Scholar 

  • Rübben H, Rathert P, Roth S, Hofstädter F, Giani G, Terhorst B, Friedrichs R (1989) Exfoliative Urinzytologie, 4th edn. Harnwegstumorregister. Publication of the Fort- und Weiterbildungskommission der Deutschen Urologen. Arbeitskreis Onkologie. Sektion Urinzytologie, Essen

    Google Scholar 

  • Sandberg AA (1986) Chromosomal changes in bladder cancer: clinical and other correlations. Cancer Genet Cytogenet 19: 163–175

    PubMed  CAS  Google Scholar 

  • Sandritter W (1981) Allgemeine Pathologic Schattauer, Stuttgart

    Google Scholar 

  • Sandritter W, Böhm N (1975) DNA in human tumors: a cytophotometric study. Curr Top Pathol 60: 151–219

    PubMed  Google Scholar 

  • Sandritter W, Carl M (1966) Cytophotometric measurement of the DNA-content (Feulgen reaction) of malignant human tumors. Acta Cytol 10: 20–30

    Google Scholar 

  • Seidel A, Sandritter W (1963) Cytophotometrische Messungen des DNS-Gehaltes eines Lungenadenoms und einer malignen Lungenadenomatose. Z Krebsforschung 65: 555–559

    CAS  Google Scholar 

  • Smeets AWGB (1987) Chromosome and flow cytometric studies of urinary bladder cancer. Dissertation, University of Maastricht

    Google Scholar 

  • Sprenger E (1985) Automation in der Zytodiagnostik. In: Deutsche Forschungs- und Versuchsanstalt für Luft- und Raumfahrt eV (DFVLR) (ed) Automation der zytologischen Diagnostik. Verlag TÜV Rheinland, Cologne, pp 7–16

    Google Scholar 

  • Sprenger W, Hilgarth M, Schaden M (1974) A follow up of doubtful findings in cervical cytology by Feulgen DNA cytophotometry. Beitr Pathol 152: 58–65

    PubMed  CAS  Google Scholar 

  • Stöckle M, Tanke HG, Mesker WE, Ploem JS, Jonas U, Hohenfellner R (1987) Automated DNA image cytometry: a prognostic tool in urinary bladder carcinoma? World J Urol 5: 127–132

    Google Scholar 

  • Summers JL, Falor WH, Ward R (1989) A 10-year analysis of chromosomes in non invasive papillary carcinoma of the bladder. J Urol 125: 177–178

    Google Scholar 

  • Tanke HJ, Ploem JS, Jonas U (1982) Kombinierte Durchflußzytometrie und Bildanalyse zur automatisierten Zytologie von Blasenepithel und Prostata. Aktuel Urol 13: 109

    Google Scholar 

  • Tanke HJ, Driel-Kulker AMJ van, Cornelisse CJ, Ploem JS (1983) Combined flow cytometry and image cytometry of the same cytological sample. J Microsc 130: 11–22

    PubMed  CAS  Google Scholar 

  • Tavares AS, Costa J, Maia JC (1973) Correlation between ploidy and prognosis in prostatic carcinoma. J Urol 109: 676–679

    PubMed  CAS  Google Scholar 

  • Tavares AS, Costa J, Carvalho A de, Reis M (1986) Tumor ploidy and prognosis in carcinomas of the bladder and prostate. Br J Cancer 20: 438–441

    Google Scholar 

  • Tribukait B, Esposti PL (1978) Quantitative flow-microfluorometric analysis of DNA in cells from neoplasms of the urinary bladder: correlation of aneuploidy with histological grading and cytological findings. Urol Res 6: 197–200

    PubMed  Google Scholar 

  • Tribukait B, Gustafson H, Esposti P (1979) Ploidy and proliferation in human bladder tumors as measured by flowcytofluorometric DNA-analysis and its relations to histopathology and cytology. Cancer 43: 1742

    PubMed  CAS  Google Scholar 

  • Vere White RW de, Deitch AD, West B, Fitzpatrick JM (1988 a) The predictive value of flow cytometric information in the clinical management of stage 0 (Ta) bladder cancer. J Urol 139: 279–282

    Google Scholar 

  • Vere White RW de, Deitch A, Strand M (1988b) DNA flow cytometry using urine samples: diagnostic accuracy. J Urol 139: 321 A

    Google Scholar 

  • Vindelov LL, Christensen IJ, Nissen NI (1983) A detergenttrypsin method for the preparation of nuclei for flow-cytometric DNA analysis. Cytometry 3: 323

    PubMed  CAS  Google Scholar 

  • Walker BE (1959) Polyploidy and differentiation in transitional epithelium of mouse urinary bladder. Chromosoma 9: 105–118

    Google Scholar 

  • Wied GL, Bartels PH, Bahr G, Oldfield DG (1968) Taxonomic intra-cellular analytic system (TICAS) for cell identification. Acta Cytol 12: 180–204

    PubMed  CAS  Google Scholar 

  • Wied GL, Bartels PH, Dytch HE, Bibbo M (1983) Rapid DNA evaluation in clinical diagnosis. Acta Cytol 27: 33–37

    PubMed  CAS  Google Scholar 

  • Wright GL, Alexander JP, Konduba AM, Schlossberg SM, Schellhammer PF (1989) Multiparameter flow-cytometric analysis of low grade, low stage bladder washings and voided urin specimens. J Urol 141: 293 A

    Google Scholar 

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Böcking, A. (1993). Diagnostic DNA Cytometry of the Urothelium. In: Urinary Cytology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76184-3_10

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  • DOI: https://doi.org/10.1007/978-3-642-76184-3_10

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