Advertisement

Purification, Identification and Regional Localization of a Brain-Specific Calretinin-Like Calcium Binding Protein (Protein 10)

  • Lois Winsky
  • David M. Jacobowitz

Abstract

We have employed two-dimensional gel electrophoresis to examine and compare the relative amounts of both known and unidentified proteins across auditory brain regions in rabbits (Winsky et al. 1989a). While several proteins were found to vary considerably in amounts between regions, one prominent protein (protein 10), having an apparent molecular mass (Mr) of 29 kDa and isoelectric point (pI) of 5.3, was found to be particularly abundant within the cochlear nucleus (Figure 1) and some other brainstem areas. In more rostral brain regions (e.g., medial geniculate and cerebral cortex) this protein was less prominent.

Keywords

Dorsal Root Ganglion Calcium Binding Protein Mossy Fiber Cochlear Nucleus Granule Cell Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Altschuler RA, Sheridan CE, Horn JW, Wenthold RJ (1989) Immunocytochemical localization of glutamate immunoreactivity in the guinea pig cochlea. Hearing Res 42:167–174.CrossRefGoogle Scholar
  2. Baimbridge KG, Miller JJ, Parkes CO (1982) Calcium-binding protein distribution in the rat. Brain Res 239:519–525.PubMedCrossRefGoogle Scholar
  3. Brodai A, Hoivik B (1964) Site and mode of termination of primary vestibulo-cerebellar fibers in the cat. An experimental study with silver impregnation methods. Arch Ital Biol 102:1–21.Google Scholar
  4. Carpenter MB (1960) Experimental anatomical-physiological studies of the vestibular nerve and cerebellar connections. In: Rasmussen GL, Widle W (eds) Neural mechanisms of the auditory and vestibular systems. CC Thomas, Springfield, pp 297–323.Google Scholar
  5. Duchesne CJ, Winsky L, Kim HM, Goping G, Vu TD, Wenthold RJ, Jacobowitz DM (1990) Identification and localization of the calcium binding protein, protein 10 in the guinea pig inner ear. Abstract to 13th meeting of the Association for Research in Otolaryngology.Google Scholar
  6. Jacobowitz DM, Palkovits M (1974) Topographical atlas of catecholamine and acetylcholinesterase-containing neurons in the brain. I. Forebrain (telencephalon, diencephalon). J Comp Neurol 157:13–28.PubMedCrossRefGoogle Scholar
  7. Lowry OH, Rosebrough NJ, Farr AL, Randall, RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275.PubMedGoogle Scholar
  8. Martin MR, Adams JC (1979) Effects of DL-alpha-amino adipate on synaptically and chemically evoked excitation of anteroventral cochlear nucleus neurons of the cat. Neurosci 4:1097–1105.CrossRefGoogle Scholar
  9. Moore JK (1986) Cochlear nuclei: relationship to the auditory nerve. In: Altschuler RA, Hoffman DW, Bobbin RP (eds) Neurobiology of hearing: The cochlea. Raven Press, New York, pp 283–301.Google Scholar
  10. Palkovits M (1973) Isolated removal of hypothalamic or other brain nuclei of the rat. Brain Res 59:449–450.PubMedCrossRefGoogle Scholar
  11. Palkovits M, Jacobowitz DM (1974) Topographic atlas of catecholamine and acetylcholinesterase-containing neurons in the rat brain. II. Hindbrain (mesencephalon, rhombencephalon). J Comp Neurol 157:29–42.PubMedCrossRefGoogle Scholar
  12. Rogers JH (1987) Calretinin: a gene for a novel calcium-binding protein expressed principally in neurons. J Cell Biol 105:1343–1353.PubMedCrossRefGoogle Scholar
  13. Rogers JH (1989a) Two calcium-binding proteins mark many chick sensory neurons. Neuroscience 31:697–709.PubMedCrossRefGoogle Scholar
  14. Rogers JH (1989b) Immunoreactivity for calretinin and other calcium binding proteins in cerebellum. Neuroscience 31:711–721.PubMedCrossRefGoogle Scholar
  15. Rogers JH, Khan M, Ellis J (1990) Calretinin and other CaBPs in the nervous system. In: Proceedings of the first European symposium on calcium binding proteins. Plenum, New York, London (in press).Google Scholar
  16. Santer DM, Heydorn WE, Creed GJ, Fukuda T, Jacobowitz DM (1988) Localization of Ca2+-binding proteins of rat cortex on two-dimensional gels-II. Analysis of Ca2+ bind proteins in ammonium sulfate raclions of rat brain. Neurochem Int 12:225–236.PubMedCrossRefGoogle Scholar
  17. Sonnenberg J, Pansini AR, Christakos S (1984) Vitamin D-dependent rat renal calcium-binding protein: development of a radioimmunoassay, tissue distribution, and immunologic identification. Endocrinology 115:640–648.PubMedCrossRefGoogle Scholar
  18. Takagi T, Nojiri M, Konishi K, Maruyama K, Nonomura Y (1986) Amino acid sequence of vitamin D-dependent calcium-binding protein from bovine cerebellum. FEBS Lett 201:41–45.PubMedCrossRefGoogle Scholar
  19. Wenthold RJ, Martin MR (1984) Neurotransmitters of the auditory nerve and central auditory system. In: Berlin C (ed) Hearing science. College Hill Press, San Diego, pp 341–369.Google Scholar
  20. Winsky L, Harvey JA, McMaster SE, Jacobowitz DM (1989a) A study of proteins in the auditory system of rabbits using two-dimensional gels: identification of glial fibrillary acidic protein and vitamin D-dependent calcium binding protein. Brain Res 496:136–146.CrossRefGoogle Scholar
  21. Winsky L, Nakata H, Jacobowitz DM (1989b) Identification of a calcium binding protein highly localized to the cochlear nucleus. Neurochem Int 15:381–389.PubMedCrossRefGoogle Scholar
  22. Winsky L, Nakata H, Martin BM, Jacobowitz DM (1989c) Isolation, partial amino acid sequence and immunohistochemical localization of a brain-specific calcium binding protein. Proc Natl Acad Sci USA 86:10139–10143.PubMedCrossRefGoogle Scholar
  23. Yamakuni T, Kuwano R, Odani S, Kiki N, Yamaguchi Y, Takahashi Y (1986) Nucleotide sequence of cDNA to mRNA for a cerebellar CaBP, spot 35 protein. Nucl Acids Res 14:6768.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Lois Winsky
  • David M. Jacobowitz

There are no affiliations available

Personalised recommendations