Purification, Identification and Regional Localization of a Brain-Specific Calretinin-Like Calcium Binding Protein (Protein 10)
We have employed two-dimensional gel electrophoresis to examine and compare the relative amounts of both known and unidentified proteins across auditory brain regions in rabbits (Winsky et al. 1989a). While several proteins were found to vary considerably in amounts between regions, one prominent protein (protein 10), having an apparent molecular mass (Mr) of 29 kDa and isoelectric point (pI) of 5.3, was found to be particularly abundant within the cochlear nucleus (Figure 1) and some other brainstem areas. In more rostral brain regions (e.g., medial geniculate and cerebral cortex) this protein was less prominent.
KeywordsCellulose Formalin Filtration Phenol Dopamine
Unable to display preview. Download preview PDF.
- Brodai A, Hoivik B (1964) Site and mode of termination of primary vestibulo-cerebellar fibers in the cat. An experimental study with silver impregnation methods. Arch Ital Biol 102:1–21.Google Scholar
- Carpenter MB (1960) Experimental anatomical-physiological studies of the vestibular nerve and cerebellar connections. In: Rasmussen GL, Widle W (eds) Neural mechanisms of the auditory and vestibular systems. CC Thomas, Springfield, pp 297–323.Google Scholar
- Duchesne CJ, Winsky L, Kim HM, Goping G, Vu TD, Wenthold RJ, Jacobowitz DM (1990) Identification and localization of the calcium binding protein, protein 10 in the guinea pig inner ear. Abstract to 13th meeting of the Association for Research in Otolaryngology.Google Scholar
- Moore JK (1986) Cochlear nuclei: relationship to the auditory nerve. In: Altschuler RA, Hoffman DW, Bobbin RP (eds) Neurobiology of hearing: The cochlea. Raven Press, New York, pp 283–301.Google Scholar
- Rogers JH, Khan M, Ellis J (1990) Calretinin and other CaBPs in the nervous system. In: Proceedings of the first European symposium on calcium binding proteins. Plenum, New York, London (in press).Google Scholar
- Wenthold RJ, Martin MR (1984) Neurotransmitters of the auditory nerve and central auditory system. In: Berlin C (ed) Hearing science. College Hill Press, San Diego, pp 341–369.Google Scholar