ISCOMS : Application in Infectious Diseases
The common problem for defined purified antigens or antigen determinants has been to make them immunogenic regardless of whether they are produced conventionally or produced as cloned gene technology products or chemically synthesized. In order to make antigen immunogenic the first problem is to get the antigen into a microscopic or submicroscopic particle where the antigen is presented in several copies, i.e. as a multimer. For some antigens it also seems necessary to enhance the immunogenicity with an adjuvant. The iscom — immunostimulating complex — was created to fulfil these criteria by assembling antigens in a multimeric form on a matrix with built-in adjuvant to form a particle. The components are held together by hydrophobic interactions (Morein et al., 1984 and for references see Höglund et al., 1989).
KeywordsInfluenza Thymidin Measle Saponin Toxicology
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- deVries, P, van Binnendijk, RS, van der Marel, P, van Wezel, AL, Voorma, HO, Sundquist, B, UytdeHaag, FGCM and Osterhaus, A (1988a). Measles virus fusion protein presented in an immune-stimulating complex (iscom) induces haemolysis-inhibiting fusion-inhibiting antibodies, virus-specific T cell clones and protection in mice. J. Gen. Virol. 69:549–559.CrossRefGoogle Scholar
- Fossum, C, Bergström, M, Lövgren, K, Watson, DL and Morein, B (1990). Effect of iscoms and their adjuvant moiety (matrix) on the initial proliferation and IL-2 responses: Comparison of spleen cells from mice inoculated with iscoms and/or matrix. Cell. Immunol., in press.Google Scholar
- Höglund, S, Dalsgaard, K, Lövgren, K, Sundquist, B, Osterhaus, A, Morein, B (1989). Iscoms and immunostimulation with viral antigens. In Subcellular Biochemistry. Ed. Harris pp39–68.Google Scholar
- Lövgren, K (1988). The serum antibody response distributed in subclasses and isotypes after intranasal and subcutaneous immunization with influenza virus immunostimulating complexes. Scand. J. Immunol. 27:241–245.Google Scholar
- Lövgren, K., Kâberg, H. and Morein, B. An experimental influenza subunit vaccine (iscom) - induction of protective immunity to challenge infection in mice after intranasal or subcutaneous administration. J. Exp. Immunol., in press.Google Scholar
- Osterhaus, ADME, deVries, P, UytdeHaag, FGCM, Visser, IKG, Morein, B (1990). Induction of protective immunity with morbillivirus iscom preparations. In Vaccines 90, Cold Spring Harbor Laboratory Press.Google Scholar
- Watson, DL, Watson, NA, Fossum, C, Lövgren, K and Morein, B. Mechanisms of immunogenicity of iscoms - evidence from antibody responses and interactions with peritoneal mononuclear leucocytes. Submitted.Google Scholar