Advertisement

ISCOMS : Application in Infectious Diseases

  • B. Morein
Conference paper

Abstract

The common problem for defined purified antigens or antigen determinants has been to make them immunogenic regardless of whether they are produced conventionally or produced as cloned gene technology products or chemically synthesized. In order to make antigen immunogenic the first problem is to get the antigen into a microscopic or submicroscopic particle where the antigen is presented in several copies, i.e. as a multimer. For some antigens it also seems necessary to enhance the immunogenicity with an adjuvant. The iscom — immunostimulating complex — was created to fulfil these criteria by assembling antigens in a multimeric form on a matrix with built-in adjuvant to form a particle. The components are held together by hydrophobic interactions (Morein et al., 1984 and for references see Höglund et al., 1989).

Keywords

Influenza Virus Rabies Virus Measle Virus Canine Distemper Virus Intranasal Application 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. deVries, P, van Binnendijk, RS, van der Marel, P, van Wezel, AL, Voorma, HO, Sundquist, B, UytdeHaag, FGCM and Osterhaus, A (1988a). Measles virus fusion protein presented in an immune-stimulating complex (iscom) induces haemolysis-inhibiting fusion-inhibiting antibodies, virus-specific T cell clones and protection in mice. J. Gen. Virol. 69:549–559.CrossRefGoogle Scholar
  2. deVries, P, UytdeHaag, F and Osterhaus, A (1988b). Canine distemper virus (CDV) immune-stimulating complexes (iscoms) but not measles virus iscoms, protect dogs against CDV infection. J. Gen. Virol. 69:2071–2983.CrossRefGoogle Scholar
  3. Fossum, C, Bergström, M, Lövgren, K, Watson, DL and Morein, B (1990). Effect of iscoms and their adjuvant moiety (matrix) on the initial proliferation and IL-2 responses: Comparison of spleen cells from mice inoculated with iscoms and/or matrix. Cell. Immunol., in press.Google Scholar
  4. Gray, D and Skarvall, H (1988). B-cell memory is short-lived in the absence of antigen. Nature 336:70–73.PubMedCrossRefGoogle Scholar
  5. Höglund, S, Dalsgaard, K, Lövgren, K, Sundquist, B, Osterhaus, A, Morein, B (1989). Iscoms and immunostimulation with viral antigens. In Subcellular Biochemistry. Ed. Harris pp39–68.Google Scholar
  6. Jones, PD, Tha Hla, R, Morein, B, Ada, GL (1988). Cellular immune response in the murine lung to local immunization with influenza A virus glycoproteins in micelles and iscoms. Scand. J. Immunol. 27:645–652.PubMedCrossRefGoogle Scholar
  7. Lövgren, K (1988). The serum antibody response distributed in subclasses and isotypes after intranasal and subcutaneous immunization with influenza virus immunostimulating complexes. Scand. J. Immunol. 27:241–245.Google Scholar
  8. Lövgren, K., Kâberg, H. and Morein, B. An experimental influenza subunit vaccine (iscom) - induction of protective immunity to challenge infection in mice after intranasal or subcutaneous administration. J. Exp. Immunol., in press.Google Scholar
  9. Morein, B, Sundquist, B, Höglund, S, Dalsgaard, K, Osterhaus, A (1984). Iscom, a novel structure for antigenic presentation of membrane proteins from enveloped viruses. Nature 308:457–460.PubMedCrossRefGoogle Scholar
  10. Osterhaus, ADME, deVries, P, UytdeHaag, FGCM, Visser, IKG, Morein, B (1990). Induction of protective immunity with morbillivirus iscom preparations. In Vaccines 90, Cold Spring Harbor Laboratory Press.Google Scholar
  11. Osterhaus, ADME and Vedder, EJ (1988). Identification of virus causing recent seal deaths. Nature 335:20.PubMedCrossRefGoogle Scholar
  12. Osterhaus, ADME, Groen, J, UytdeHaag, FGCM, Visser, IKG, van der Bildt, MWG, Bergman, A and Klingeborn, B (1989). Distemper virus in Baikal seals. Nature 338:209.CrossRefGoogle Scholar
  13. Speijers, GJA, Danse, LHJC, Beuvery, JJTW, Strik, A and Vos, JG (1988). Local reactions of the Saponin Quil A and Auil A containing iscom measles vaccine after intramuscular injection of rats: A comparison with the effect of DPT-polio vaccine. Fund. Appl. Toxicol. 10:425–430.CrossRefGoogle Scholar
  14. Takahashi, H, Takeshita, T, Morein, B, Putney, S, Germain, RN, Berzofsky, JA (1990). Induction of specific CD8+ class I-MHC restricted CTL against HIV envelope protein by immunization with purified whole protein in iscoms. Nature 344:873–875.PubMedCrossRefGoogle Scholar
  15. Ulaeto, D, Wallace, L, Morgan, A, Morein, B, Rickinson, A (1988). In vitro T cell reponsees to a candidate Epstein-Barr virus vaccine: human CD4 T cell clones specific for the major envelope glycoprotein gp340. Eur. J. Immunol. 18:1689–1697.PubMedCrossRefGoogle Scholar
  16. Watson, DL, Lövgren, K, Watson, NA, Fossum, C, Morein, B and Höglund, S (1989). Inflammatory response and antigen localization following immunization with influenza virus iscoms. Inflammation 13:641–649.PubMedCrossRefGoogle Scholar
  17. Watson, DL, Watson, NA, Fossum, C, Lövgren, K and Morein, B. Mechanisms of immunogenicity of iscoms - evidence from antibody responses and interactions with peritoneal mononuclear leucocytes. Submitted.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • B. Morein
    • 1
  1. 1.Department of Virology, National Veterinary Institute and SwedishUniversity of Agricultural Sciences, Biomedical CentreUppsalaSweden

Personalised recommendations