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The Hyphal Tip Cell of Sclerotium Rolfsii: Cytological Observations

  • R. W. Roberson
Part of the NATO ASI Series book series (ASIH, volume 53)

Abstract

The hyphal apex is a highly specialized region of the fungal cell where polarized growth and differentiation occur. Numerous cellular activities are involved in maintaining apical growth and morphogenesis in fungi. Among these are the apical migration of cytoplasmic vesicles transporting materials used for plasma membrane and wall formation, the incorporation of these vesicles into the Spitzenkorper in higher fungi, the positioning and movement of the Spitzenkorper in the apex, and the final delivery of vesicles from the Spitzenkorper to the apical plasma membrane. How the cell regulates and coordinates these events during hyphal growth and development is not totally clear, although it is assumed that the cytoskeleton is primarily involved (Bartnicki- Garcia et al., 1989; Howard and Aist, 1980; McKerracher and Heath, 1987; Tucker et al., 1986; Wessels, 1986).

Keywords

Apical Region Multivesicular Body Apical Growth Hyphal Cell Schizophyllum Commune 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bartnicki-Garcia S, Hergert F, Gierz G. (1989) Computer simulation of fungal morphogenesis and the mathematical basis for hyphal (tip) growth. Protoplasma 153: 46–57CrossRefGoogle Scholar
  2. Bartnicki-Garcia S, Hergert F., Gierz G. (1990) A novel computer model for generation cell shape: application to fungal morphogenesis. In: Kuhn PJ, Trinci APJ, Jung MJ, Goosey MW, Copping LG (eds) Biochemistry of cell walls and membranes in fungi. Springer-Verlag, Berlin Heidelberg, pp 43–60CrossRefGoogle Scholar
  3. Bartnicki-Garcia S, Lippman E. (1969) Fungal morphogenesis: cell wall construction in Mucor rouxii. Science 165: 302–304PubMedCrossRefGoogle Scholar
  4. Fuller MS, Roberson RW, Gisi U (1990) Effects of the demethylase inhibitor, cyproconazole, on hyphal tip cells of Sclerotium rolfsii III. Cell wall cytochemistry. Pestic Biochem Physiol 36: 115–126Google Scholar
  5. Girbardt M (1957) Der Spitzenkorper von Polystictus versicolor (L.). Planta 50: 47–59CrossRefGoogle Scholar
  6. Girbardt M (1969) Die ultrastruktur der apikalreregion von pilzhyphen. Protoplasma 67: 413–441CrossRefGoogle Scholar
  7. Gooday GW (1971) An autoradiographic study of hyphal growth of some fungi. J Gen Microbiol 67: 125–133CrossRefGoogle Scholar
  8. Grove SN (1978) The cytology of hyphal tip growth. In: Smith JE, Berry DR (eds) The filamentous fungi, vol III. Wiley, New York, p 28Google Scholar
  9. Grove SN, Bracker CE (1970) Protoplasmic organization of hyphal tips among fungi• vesicles and Spitzenkorper. J Bacteriol 104: 989–1009.PubMedCentralPubMedGoogle Scholar
  10. Grove SN, Sweigard JA (1980) Cytochalasin A inhibits spore germination and hyphal tip growth in Gilbertella persicaria. Exp Mycol 4: 239–250CrossRefGoogle Scholar
  11. Heath IB (1987) Preservation of a labile cortical array of actin filaments in growing hyphal tips of the fungus Saprolegnia ferax. Eur J Cell Bio 44: 10–16Google Scholar
  12. Herr FB, Heath MC (1982) The effects of antimicrotubular agents on organelle positioning in the rust fungus, Uromyces phaseoli var. vaignae. Exp Mycol 6: 15–24CrossRefGoogle Scholar
  13. Hoch HC, Howard RJ (1980) Ultrastructure of freeze-substituted hyphae of the basidiomycete Laetisaria arvalis. Protoplasma 103: 281–297CrossRefGoogle Scholar
  14. Hoch HC, Staples RC (1983a) Ultrastructural organization of the nondifferentiated uredospore germling of Uromyces phaseoli variety typica. Mycologia 75: 795–824CrossRefGoogle Scholar
  15. Hoch HC, Staples RC (1983b) Vizualisation of actin by rhodamine-conjugated phalloidin in the fungus Uromyces phaseoli. Eur J Cell Bio! 32: 52–58Google Scholar
  16. Hoch HC, Staples RC (1985) The microtubule cytoskeleton in hyphae of Uromyces phaseoli germlings: Its relationship to the region of nucleation and to the F-actin cytoskeleton. Protoplasma 124: 112–122.Google Scholar
  17. Howard RJ (1981) Ultrastructural analysis of hyphal tip cell growth in fungi: Spitzenkorper, cytoskeleton and endomembranes after freeze-substitution. J Cell Sci 48: 89–103PubMedGoogle Scholar
  18. Howard RJ, Aist JR (1979) Hyphal tip cell ultrastructure of the fungus Fusarium: improved preservation by freeze-substitution. J Ultrastruct Res 66: 224–234PubMedCrossRefGoogle Scholar
  19. Howard RJ, Aist JR (1980) Cytoplasmic microtubules and fungal morphogenesis: Ultrastructural effects of methyl benzimidazole-2-ylcarbamate determined by freeze-substitution of hyphal tip cells. J Cell Bio 87: 55–64CrossRefGoogle Scholar
  20. McKerracher LI, Heath IB (1987) Cytoplasmic migration and intracellular organelle movements during tip growth of fungal hyphae. Exp Mycol 11: 79–100CrossRefGoogle Scholar
  21. Mims CW, Richardson EA (1989) Ultrastructure of appressorium development by basidiospore germlings of the rust fungus Gyrnnosporangium juniperi-virginianae. Protoplasma 148: 111–119CrossRefGoogle Scholar
  22. Raudaskoski M, Salo V, Niini SS (1988) Structure and function of the cytoskeleton in filamentous fungi. Karstenia 28: 49–60Google Scholar
  23. Roberson RW, Fuller MS (1988) Ultrastructural aspects of the hyphal tip of Sclerotium rolfsii preserved by freeze substitution. Protoplasma 146: 143–149CrossRefGoogle Scholar
  24. Roberson RW, Fuller MS (1990) Effects of the demethylase inhibitor, cyproconazole, on hyphal tip cells of Sclerotium rolfsii II. An electron microscope study. Exp Mycol 14: 124–135Google Scholar
  25. Roberson RW, Fuller MS, Grabski C (1989) Effects of the demethylase inhibitor, cyproconazole, on hyphal tip cells of Sclerotium rolfsii I. A light microscope study. Pestic Biochem Physiol 34: 130–142Google Scholar
  26. Runeberg P, Raudaskoski M (1986) Cytoskeletal elements in the hyphae of the homobasidiomycete Schizophyllum commune visualized with indirect immunofluorescence and NBC-phallacidin. Eur J Cell Biol 41: 25–32Google Scholar
  27. van Tuinen D, Perez RO, Turian G (1986) A search for myosin in elongating hyphae of Neurospora crassa. Botanica Helvetica 96: 299–302Google Scholar
  28. Tucker BE, Hoch HC, Staples RC (1986) The involvement of F actin in Uromyces cell differentiation. The effects of cytochalasin E and phalloidin. Protoplasma 135: 88–101Google Scholar
  29. Watts FZ, Miller DM, Orr E (1985) Identification of myosin heavy chain in Saccharomyces cerevisiae. Nature 316: 83–85PubMedCrossRefGoogle Scholar
  30. Wessels JGH (1986) Cell wall synthesis in apical hyphal growth. Inter Rev Cytol 104: 37–79CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • R. W. Roberson
    • 1
  1. 1.Arizona State UniversityTempeUSA

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