Abstract
Electron microscopy and since about two decades image processing are techniques that have contributed much to our understanding of the structure of microbial cells and their constitutents. In the beginning of electron microscopy whole cells or isolated cell walls were rendered visible in the microscope, followed by the extensive investigation of thin sections which is still an indispensible technique in cytology. Nowadays, after much effort has been spent into the development of protective sample preparation, image processing and reconstruction techniques, electron microscopy is on the way to near atomic resolution of biological macromolecules (Henderson et al., 1990; Sass et al., 1989; Jap et a1.,1990). While the determination of the complete 3-D structure of proteins is still a challenge for electron microscopists, the reconstruction of proteins on the domain structure level has become a well established technique in the recent years (Glaeser, 1985; Frank, 1989).
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© 1991 Springer-Verlag Berlin Heidelberg
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Engelhardt, H. (1991). Electron Microscopy of Microbial Cell Wall Proteins. Surface Topography, Three-Dimensional Reconstruction, and Strategies for Two-Dimensional Crystallization. In: Latgé, J.P., Boucias, D. (eds) Fungal Cell Wall and Immune Response. NATO ASI Series, vol 53. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76074-7_2
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DOI: https://doi.org/10.1007/978-3-642-76074-7_2
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