Mouse Monoclonal Antibodies Against Candida Albicans and Cryptococcus Neoformans

  • J.-M. Bastide
Conference paper
Part of the NATO ASI Series book series (ASIH, volume 53)


Specific antibodies are necessary for the rapid identification of pathogenic yeasts, the elucidation of the structure of serotypic specific epitopes, and the detection of circulating antigens in patients with disseminated mycoses. Monoclonal antibodies (MAbs) are presently the most efficient probe for these purposes. This review presents the main results on the production and characterization of monoclonal antibodies directed against Candida albicans and Cryptococcus neoformans which are responsible for the major yeast infections in immunocompromised patients.


Candida Albicans Candida Species Germ Tube Cryptococcus Neoformans Capsular Polysaccharide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Axelsen NH (1973) Quantitative immunoelectrophoretic methods as tools for a polyvalent approach to standardization in the immunochemistry of Candida albicans. Infect Immun 7: 949–960PubMedCentralPubMedGoogle Scholar
  2. Brawner DL, Cutler JE (1984) Variability in expression of a cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody. Infect Immun 43: 966–972PubMedCentralPubMedGoogle Scholar
  3. Brawner DL, Cutler JE (1986) Variability in expression of a cell surface antigen of Candida albicans during morphogenesis. Infect Immun 51: 337–343PubMedCentralPubMedGoogle Scholar
  4. Casanova M, Gil ML, Cardenoso L, Martinez JP, Sentandreu R (1989) Identification of wall-specific antigens synthesized during germ tube formation by Candida albicans. Infect Immun 57: 262–271PubMedCentralPubMedGoogle Scholar
  5. Casanova M, Martinez JP, La Jean Chaffin W (1990) Fab fragments from a monoclonal antibody against a germ tube mannoprotein block the yeast to mycelium transition in Candida albicans. Infect Immun 58: 3810–3812PubMedCentralPubMedGoogle Scholar
  6. Eckert TF, Kozel TR (1987) Production and characterization of monoclonal antibodies specific for Cryptococcus neoformans capsular polysaccharide. Infect Immun 55: 1895–1899PubMedCentralPubMedGoogle Scholar
  7. Cherniak R, Reiss E, Turner SH (1982) A galactoxylomannan antigen of Cryptococcus neoformans serotype A. Carbohydr Res 103: 239–250CrossRefGoogle Scholar
  8. Dromer F, Charreire J, Contrepois A, Carbon C, Yeni P (1987) Protection of mice against cryptococcosis by anti-Cryptococcus neoformans monoclonal antibody. Infect Immun 55: 749–752PubMedCentralPubMedGoogle Scholar
  9. Eckert TF, Kozel TR (1987) Production and characterization of monoclonal antibodies specific for Cryptococcus neoformans capsular polysaccharide. Infect Immun 55: 1895–1899PubMedCentralPubMedGoogle Scholar
  10. Fortier B, Hopwood V, Poulain D (1988) Electric and chemical fusions for the production of monoclonal antibodies reacting with the in vivo growth phase of Candida albicans. J Med Microbiol 27: 239–245PubMedCrossRefGoogle Scholar
  11. Fukazawa Y, Shinoda T, Nishikawa A, Kagaya K, Yonezawa Y (1976) Serological and immunological studies of the serotypes of Candida albicans. In: Iwata K (Ed), Yeasts and yeast-like microorganisms in medical science. Tokyo University Press, Tokyo, p 213Google Scholar
  12. Hall NK, Blackstock R (1981) Production of specific antibody to Cryptococcus neofonnans by hybridomas in vitro. Sabouraudia 19: 157–160PubMedCrossRefGoogle Scholar
  13. Ikeda R, Nishikawa A, Shinoda T, Fukazawa Y (1985) Chemical characterization of capsular polysaccharide from Cryptococcus neofonnans serotype A-D. Microbiol Immunol 29: 981–985PubMedCrossRefGoogle Scholar
  14. Ikeda R, Shinoda R, Fukazawa Y, Kaufman L (1982) Antigenic characterization of Cryptococcus neoformans serotypes and its application to serotyping of clinical isolates. J Clin Microbiol 16: 22–29PubMedCentralPubMedGoogle Scholar
  15. Lebecq JC, Salhi SL, Bastide JM (1984) A novel antibody overlay technique for two-dimensional immunoelectrophoresis. J Immunol Methods 66: 219–226PubMedCrossRefGoogle Scholar
  16. Miyakawa Y, Kagaya K, Fukazawa Y, Soe G (1986) Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans. J Clin Microbiol 23: 881–886.PubMedCentralPubMedGoogle Scholar
  17. Munoz C, Mazie JC, Delga JM, Dupont B, Latgé JP, Drouhet E (1988) Monoclonal antibodies anti-Candida albicans mannan and anti Aspergillus fumigatus galactomannan. In: Drouhet E, Cole GT, de Repentigny L, Latgé JP, Dupont B (Eds) Fungal antigens. Plenum Press, New York London, p 213CrossRefGoogle Scholar
  18. Polonelli L, Morace G (1986) Specific and common antigenic determinants of Candida albicans isolates detected by monoclonal antibody. J Clin Microbiol 23: 366–368PubMedCentralPubMedGoogle Scholar
  19. Reiss E, Cherniak R, Eby R, Kaufman L (1984) Enzyme immunoassay detection of IgM to galactoxylomannan of Cryptococcus neofonnans. Diag Immunol 2: 109–115Google Scholar
  20. Reiss E, de Repentigny L, Kuykendall RJ, Carter AW, Galindo R, Auger P, Bragg SL, Kaufman L (1986) Monoclonal antibodies against Candida tropicalis mannan• antigen detection by enzyme immunoassay and immunofluorescence. J Clin Microbiol 24: 796–802PubMedCentralPubMedGoogle Scholar
  21. Salhi SL, Bastide JM (1988) Immunochemistry of Candida albicans antigens. In: Drouhet E, Cole GT, De Repentigny L, Latgé JP, Dupont B (Eds) Fungal antigens. Plenum Press, New York London, p 141CrossRefGoogle Scholar
  22. Sanford JE, Lupan DM, Schlageter AM, Kozel TR (1990) Passive immunization against Cryptococcus neoformans with an isotype-switch family of monoclonal antibodies reactive with cryptococcal polysaccharide. Infect Immun 58: 1919–1923PubMedCentralPubMedGoogle Scholar
  23. Schlageter AM, Kozel TR (1990) Opsonization of Cryptococcus neoformans by a family of isotype-switch variant antibodies specific for the capsular polysaccharide. Infect Immun 58: 1914–1918PubMedCentralPubMedGoogle Scholar
  24. Shinoda T, Suzuki Y, Ikeda R, Nishikawa A (1988) Production and serological characterization of monoclonal antibodies against Cryptococcus neofonnans serotype A capsular polysaccharide. Xth Congress of the ISHAM, Barcelona, July 1988, Abstract P-214Google Scholar
  25. Spiropulu C, Eppard RA, Otteson E, Kozel TR (1989) Antigenic variation within serotypes of Cryptococcus neoformans detected by monoclonal antibodies specific for the capsular polysaccharide. Infect Immun 57: 3240–3242PubMedCentralPubMedGoogle Scholar
  26. Strockbine NA, Largen MT, Zweibel SM, Buckley HR (1984a) Identification and molecular weight characterization of antigens from Candida albicans that are recognized by human sera. Infect Immun 43: 715–721PubMedCentralPubMedGoogle Scholar
  27. Strockbine NA, Largen MT, Buckley HR (1984b) Production and characterization of three monoclonal antibodies to Candida albicans proteins. Infect Immun 43: 1012–1018PubMedCentralPubMedGoogle Scholar
  28. Sundstrom PM, Tam MR, Nichols ET, Kenny GE (1988) Antigenic differences in the surface mannoproteins of Candida albicans as revealed by monoclonal antibodies. Inf Immun 56: 601–606Google Scholar
  29. Todaro-Luck F, Reiss E, Cherniak R, Kaufman L (1989) Characterization of Cryptococcus neoformans capsular glucuronoxylomannan polysaccharide with monoclonal antibodies. Infect Immun 57: 3882–3887PubMedCentralPubMedGoogle Scholar
  30. Tojo M, Shibata N, Kobayashi M, Mikami T, Suzuki M, Suzuki S (1988) Preparation of monoclonal antibodies reactive with ß-1,2-linked oligomannosyl residues in the phosphomannan-protein complex of Candida albicans NIH.B-792 strain. Clin Chem 34: 539–543PubMedGoogle Scholar
  31. Tsuchiya T, Kawakita S, Udagawa M (1963) Rapid identification of Cryptococcus neoformans by serology. Sabouraudia 2: 209–214CrossRefGoogle Scholar
  32. Tsuchyia T, Fukazawa Y, Taguchi M, Nakase T, Shinoda T (1974) Serologic aspects of yeast classification. Mycopathol Mycol Appl 53: 77–91CrossRefGoogle Scholar
  33. Turner SH, Cherniak R, Reiss E (1984) Fractionation and characterization of galactoxylomannan from Cryptococcus neofonnans. Carbohydr Res 125: 343–349PubMedCrossRefGoogle Scholar
  34. Van de Moer A, Salhi SL, Cherniak R, Pau B, Garrigues ML, Bastide JM (1990) An anti-Cryptococcus neoformans monoclonal antibody directed against galactoxylomannan. Res Immunol 141: 33–42PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • J.-M. Bastide
    • 1
  1. 1.University of Montpellier IMontpellierFrance

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