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Extracellular Polysaccharides of Moulds and Their Immunological Activity

  • H. J. Kamphuis
  • S. H. W. Notermans
  • G. H. Veeneman
  • J. H. van Boom
  • F. M. Rombouts
Part of the NATO ASI Series book series (ASIH, volume 53)

Abstract

It has been known for a long time that fungi secrete during their growth extra-polysaccharides. Fungal extrapolysaccharides (EPS) can induce the production of specific antibodies after injection into hyperimmunized experimental animals. In humans specific antibodies may be produced either after passive exposure to spores or in response to parasitic development. There is great deal of heterogeneity in the antibody combining sites present on polysaccharidic antigens (Sutherland,1977; Wu, 1988). Fungal genera have been discriminated using antibodies specific to EPS (Notermans and Soentoro, 1986). Alternatively, cross reactivity is often found between fungal species on the basis of antigen-antibodies reactions. Since fungal EPS may provide a basis for immunological detection of fungi in food or infected persons, more information is needed about the relationship between chemical composition of EPS and their specific antibodies. In this chapter, production, purification, characterization and antigenic specificity of mould antigens are presented with special emphasis on Aspergillus and Penicillium EPS.

Keywords

Invasive Aspergillosis Sandwich ELISA Extracellular Polysaccharide Immunological Detection Inhibition ELISA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • H. J. Kamphuis
    • 1
  • S. H. W. Notermans
    • 1
  • G. H. Veeneman
    • 2
  • J. H. van Boom
    • 2
  • F. M. Rombouts
    • 3
  1. 1.National Institute of Public Health and Environmental ProtectionBilthovenThe Netherlands
  2. 2.Gorlaeus LaboratoriaLeidenThe Netherlands
  3. 3.Wageningen Agricultural UniversityWageningenThe Netherlands

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